Team:Calgary/Notebook/Safety And Protocols/Comments

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Revision as of 03:15, 7 September 2010

Comments on the Protocols

Safety and Protocols Main Page

Taq Polymerase Chain Reaction

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Transformable Competent Cell Induction

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Bacterial Transformation

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Restriction Digest

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Ligation

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Miniprep Plasmid Preparation (using GenElute)

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Agarose Gel Electrophoresis

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Making of Agarose Gels

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Rehydration of Registry DNA

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Construction Technique

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Overnight Cultures

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Glycerol Stock Preparation

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LB Agar Plate Preparation

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QIAprep Spin Miniprep Kit

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Gel Extraction

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PCR Purification

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