Team:Calgary/9 August 2010


Revision as of 20:46, 9 August 2010 by H.dastidar (Talk | contribs)

Monday August 9, 2010


Today I did a construction of I0500-B0034 with MalE31 that was biobricked by Emily. I cut I0500-B0034 (pSB1AC3) with XbaI and PstI and I cut MalE31( psb1AK3) with SpeI and PstI. This disgest was followed by phosphatasing the vector and ligating the two parts with qucik ligase. I plated on AC to select for the cells which have the vectors.

I also read some more papers regarding the relationship between pH, temperature and inclusion body formation. I also went through some tutorials for MATLAB in order to help me define parameters and equation that will make the actual model.

No notebook page exists for this date. Sorry!