Monday August 9, 2010

Himika

Today I did a construction of I0500-B0034 with MalE31 that was biobricked by Emily. I cut I0500-B0034 (pSB1AC3) with XbaI and PstI and I cut MalE31( psb1AK3) with SpeI and PstI. This disgest was followed by phosphatasing the vector and ligating the two parts with qucik ligase. I plated on AC to select for the cells which have the vectors.

I also read some more papers regarding the relationship between pH, temperature and inclusion body formation. I also went through some tutorials for MATLAB in order to help me define parameters and equation that will make the actual model.

Jeremy

Today I picked up the stuff I did over the weekend. Which included ligating, transforming and plated the bacteria for the pRFP plasmid switch of pSB1AK3 and pSB1AC3. I am also doing the restriction enzyme again this time with the right NEB buffer for the plasmid switch. This also included antarctic phosphatase and am leaving in the freezer until tomorrow. I also finalized the t-shirt print ready designs, all we have to do now is contact a t-shirt company. And briefly fixed several of the planned wiki page. Some of my time also went towards looking over our mascot costume that came in today.