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Team:Calgary/9 August 2010
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'''Monday August 9, 2010''' | '''Monday August 9, 2010''' | ||
| - | + | <u>Himika</u> | |
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| + | Today I did a construction of I0500-B0034 with MalE31 that was biobricked by Emily. I cut I0500-B0034 (pSB1AC3) with XbaI and PstI and I cut MalE31( psb1AK3) with SpeI and PstI. This disgest was followed by phosphatasing the vector and ligating the two parts with qucik ligase. I plated on AC to select for the cells which have the vectors. | ||
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| + | I also read some more papers regarding the relationship between pH, temperature and inclusion body formation. I also went through some tutorials for MATLAB in order to help me define parameters and equation that will make the actual model. | ||
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Revision as of 20:46, 9 August 2010
Monday August 9, 2010
Himika
Today I did a construction of I0500-B0034 with MalE31 that was biobricked by Emily. I cut I0500-B0034 (pSB1AC3) with XbaI and PstI and I cut MalE31( psb1AK3) with SpeI and PstI. This disgest was followed by phosphatasing the vector and ligating the two parts with qucik ligase. I plated on AC to select for the cells which have the vectors.
I also read some more papers regarding the relationship between pH, temperature and inclusion body formation. I also went through some tutorials for MATLAB in order to help me define parameters and equation that will make the actual model.
No notebook page exists for this date. Sorry!
