http://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&feed=atom&action=historyTeam:Calgary/8 July 2010 - Revision history2024-03-28T17:39:26ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=55520&oldid=prevPjwu at 03:36, 23 August 20102010-08-23T03:36:44Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Thursday July 8, 2010|</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1 & C2.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies C1 and C2 into pSB1AK3]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1 & C2.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies C1 and C2 into pSB1AK3]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1D & C2D.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies D C1 and D C2 into pSB1AK3]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1D & C2D.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies D C1 and D C2 into pSB1AK3]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010.R0040+I13504.jpg|thumb|400px| Plates containing colonies of Succesful TetRPromoter-GFP construct]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010.R0040+I13504.jpg|thumb|400px| Plates containing colonies of Succesful TetRPromoter-GFP construct]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Thursday July 8, 2010'''</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Jeremy</u></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Jeremy</u></div></td></tr>
</table>Pjwuhttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=28254&oldid=prevEmily Hicks at 16:36, 20 July 20102010-07-20T16:36:12Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We are still waiting on the new reverse primer for the cpxp promoter. The RFP reporter device was constructed with a tetR promoter to test if it is working. Today I worked on updating the list of media outlets we will be contacting once the media relation’s contacts return from vacation. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We are still waiting on the new reverse primer for the cpxp promoter. The RFP reporter device was constructed with a tetR promoter to test if it is working. Today I worked on updating the list of media outlets we will be contacting once the media relation’s contacts return from vacation<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><u>Emily</u></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Today we spent time talking about what we're going to present tomorrow in the meeting with our supervisors. We talked about the project as a whole, the general aims as well as the status of some of the sub projects. We designed powerpoint slides and planned out what each individual is going to say in the presentation tomorrow. Today I also spent some time editing the letter that we're writing to the president of the University</ins>.</div></td></tr>
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</table>Emily Hickshttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=24830&oldid=prevAjgrigg at 17:11, 16 July 20102010-07-16T17:11:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I worked on the construction of R0040+I13504 in order to test the composite part I13504. This part will be used at the end of the translation/transcription circuit.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I worked on the construction of R0040+I13504 in order to test the composite part I13504. This part will be used at the end of the translation/transcription circuit.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><u>Alex</u></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We are still waiting on the new reverse primer for the cpxp promoter. The RFP reporter device was constructed with a tetR promoter to test if it is working. Today I worked on updating the list of media outlets we will be contacting once the media relation’s contacts return from vacation. </ins></div></td></tr>
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</table>Ajgrigghttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=20040&oldid=prevPjwu at 20:08, 12 July 20102010-07-12T20:08:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Thursday July 8, 2010'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Thursday July 8, 2010'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Results:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Results:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plates from the plasmid switch grew! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plates from the plasmid switch grew! </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Raida</u> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Raida</u> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Chris</u> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Chris</u> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><u>Dev<del class="diffchange diffchange-inline">:</del></u> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><u>Dev</u> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I worked on the construction of R0040+I13504 in order to test the composite part I13504. This part will be used at the end of the translation/transcription circuit.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I worked on the construction of R0040+I13504 in order to test the composite part I13504. This part will be used at the end of the translation/transcription circuit.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">}}</del></div></td><td colspan="2"> </td></tr>
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</table>Pjwuhttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=19837&oldid=prevDvyas at 16:13, 12 July 20102010-07-12T16:13:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><u>Dev:</u> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Today, I worked on the construction of R0040+I13504 in order to test the composite part I13504. This part will be used at the end of the translation/transcription circuit.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td></tr>
</table>Dvyashttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=19806&oldid=prevCktang at 15:59, 12 July 20102010-07-12T15:59:07Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><u>Jeremy<del class="diffchange diffchange-inline">:</del></u>Today I worked on a construction of R0040+I13507</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><u>Jeremy</u></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Today I worked on a construction of R0040+I13507</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct is used as a test for the I13507 portion which should include RBS (B0034), GFP (E0040) and Terminator (B0015) which will be included in our protein misfolding circuit</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct is used as a test for the I13507 portion which should include RBS (B0034), GFP (E0040) and Terminator (B0015) which will be included in our protein misfolding circuit</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plates from the plasmid switch grew! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plates from the plasmid switch grew! </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><u>Raida<del class="diffchange diffchange-inline">:</del></u> Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><u>Raida</u> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><u>Chris</u> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><u>Chris:</u> </del>Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td></tr>
</table>Cktanghttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=17827&oldid=prevCktang at 23:08, 8 July 20102010-07-08T23:08:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Raida:</u> Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><u>Raida:</u> Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><u>Chris:</u> Today, I spent time fixing up a couple pages on the wiki Notebook as well as working on the powerpoint presentation that we are supposed to do for Dr. Schryvers, Dr. Logan, and Deirdre tomorrow. A schedule was received from our Suffield contact Les Nagata and we began planning our visit. Finally, I continued with the emails to Joe Astroth, Sigma Aldrich, and Dr. Elizabeth Cannon.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td></tr>
</table>Cktanghttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=17534&oldid=prevRaidakhwaja at 17:43, 8 July 20102010-07-08T17:43:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1 & C2.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies C1 and C2 into pSB1AK3]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1 & C2.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies C1 and C2 into pSB1AK3]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1D & C2D.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies D C1 and D C2 into pSB1AK3]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1D & C2D.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies D C1 and D C2 into pSB1AK3]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:07.08.2010.R0040+I13504.jpg|thumb|400px| Plates containing colonies of Succesful TetRPromoter-GFP construct]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Thursday July 8, 2010'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Thursday July 8, 2010'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Results:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Results:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plates from the plasmid switch grew! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plates from the plasmid switch grew! </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><u>Raida:</u> Today I checked the Plates that grew overnight with my TetRPromoter-GFP construct. Because both the insert and the vector were Ampicilin Resistant, the only successful constructions were those that glew Green under UV light. Plates 3,5 and 6 shows only Green colonies under the UV, but interestingly, Plates 1 and 4 show both Green AND Red colonies. This could mean two things: 1, that there is some kind of contamination in those plates and 2. There were unsuccessful plasmid switch of the GFP construct into an Ampicilin Resistant Plasmid. Either way, Jeremey and Dev are going to re-do the construction to verify the Results. As of now, the successful construction plates will be stored in the freezer. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>}}</div></td></tr>
</table>Raidakhwajahttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=17516&oldid=prevJdchoo at 17:24, 8 July 20102010-07-08T17:24:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{CalgaryNotebookTemplate|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{CalgaryNotebookTemplate|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1 & C2.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies C1 and C2 into pSB1AK3]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1 & C2.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies C1 and C2 into pSB1AK3]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:07.08.2010. I0500 plasmid switch into pSB1AK3 C1D & C2D.jpg|thumb|400px|Jeremy's I0500 plasmid switch for colonies D C1 and D C2 into pSB1AK3]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Thursday July 8, 2010'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Thursday July 8, 2010'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Jdchoohttp://2010.igem.org/wiki/index.php?title=Team:Calgary/8_July_2010&diff=17511&oldid=prevJdchoo at 17:22, 8 July 20102010-07-08T17:22:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''</del>Jeremy:<del class="diffchange diffchange-inline">'''</del>Today I worked on a construction of R0040+I13507</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><u></ins>Jeremy:<ins class="diffchange diffchange-inline"></u></ins>Today I worked on a construction of R0040+I13507</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct is used as a test for the I13507 portion which should include RBS (B0034), GFP (E0040) and Terminator (B0015) which will be included in our protein misfolding circuit</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This construct is used as a test for the I13507 portion which should include RBS (B0034), GFP (E0040) and Terminator (B0015) which will be included in our protein misfolding circuit</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Jdchoo