Emily

Today I PCR Purified malE and malE31. I then digested these as well as the psB1AK3 vector with a combination of restriction enzymes to try to get it into an AK BBK construction vector. I ligated these and transformed them into TOP10 competant cells and plated on K plates, leaving for overnight growth. I also made more K plates as well as more AK broth. Today we also all worked on the presentation that we will be giving at the 3rd annual aGEM Jamboree this coming weekend in Edmonton.