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Team:Calgary/30 September 2010
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I did another restriction digest of I0500-B0034-MalE since the restriction digest last night failed. This time I tried with EcoRI and SpeI. The restriction digest worked and Colony 10 will be sent down for sequencing tomorrow. The plates from last night did not grow either. So I retried the construction by retrying the restriction digest. I left the digest overnight and will plate tomorrow. | I did another restriction digest of I0500-B0034-MalE since the restriction digest last night failed. This time I tried with EcoRI and SpeI. The restriction digest worked and Colony 10 will be sent down for sequencing tomorrow. The plates from last night did not grow either. So I retried the construction by retrying the restriction digest. I left the digest overnight and will plate tomorrow. | ||
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| + | [[Image:09.28.2010-I0500-B0034-MALE rd.jpg|thumb|400px|Restriction digest of I0500-B0034-MalE colonies 1-12, Lane 1 is control]] | ||
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Revision as of 16:48, 4 October 2010
Thursday September 30, 2010
Himika
I did another restriction digest of I0500-B0034-MalE since the restriction digest last night failed. This time I tried with EcoRI and SpeI. The restriction digest worked and Colony 10 will be sent down for sequencing tomorrow. The plates from last night did not grow either. So I retried the construction by retrying the restriction digest. I left the digest overnight and will plate tomorrow.
