Team:Calgary/25 October 2010
From 2010.igem.org

Monday October 25, 2010
Patrick
Assigned and obtained various paragraphs from other team members. Uploaded many on the Wiki.
Emily
Set up digests of cpxP, malE, malESSdel, I0500-I13507, K135000-I13507-I0500-B0034-malE31 and the psB1AC3 vector. This is to plasmid switch all of the remianing registry parts into the accepted shipping plasmid. After construction and Antarctic Phosphotase treatment, I ligated them and then transformed them into TOP10 Competant E. Coli cells and then innocultaed LB broth culture and left the cultures to grow overnight. Today I also preparted ovenright cultures for an arabinose induction in order to get some more characterizatuon data of the I0500 (arabinose inducible) promoter. I also entered our parts into the Registry of Standard Biological Prats in preparation for shipping tomorrow.