Team:Calgary/22 July 2010

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Thursday July 22, 2010

Himika

Today I miniprepped 10 colonies from the I0500-B0034 construction using Sigma-Aldrich kit. I also did a plasmid switch of I0500-B0034 from pSB1A3 to pSB1AC3.

Jeremy

Today I did a colony PCR of K23900+I13507, K239000+I13504, K135000+I13507, K135000+I13504 and prepared an overnight of several colonies. I also re-ligated and transformed I0500+B0034 in pSB1AC3 plasmid.

Chris

Today, I calculated the values of the enzymes we received from New England Biolabs. As well, I redid the gel of the colony PCR of the CpxP promoter with a 1.0% gel. This time, the DNA actually moved and the picture of the gel can be seen on the right. However, the bands were very non-specific. To optimize, I set up one gradient PCR with temperature of annealing step ranging from 53-58°C.

No notebook page exists for this date. Sorry!