Team:Calgary/21 July 2010

From 2010.igem.org

Revision as of 21:46, 21 July 2010 by Raidakhwaja (Talk | contribs)

Wednesday July 21, 2010

Raida:

Objective: PCR out native MAlE/ MalE 31 protein out of E.coli Purpose: Testing Mal E/ Mal E31 primers to get 1188 bp long gene of interest. Primers that are used are gene specific and anneals right on the start of the Mal E gene

Method: Preparing the Master Mix {

- ! Reagent ! x1μL ! x8μL