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Team:Calgary/20 August 2010
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| + | Today I PCR purified malE and malE31. I then set up a digest of these, plus the psB1AK3 with a combination of enzymes: both EcoRI/SpeI and XbaI/PstI. This is to try to get the linearized PCR product into the AK BBK Construction plasmid. I then transformed these into TOP10 cells and plated them on AK plates. Today we also had a meeting in order to finalize some details for the iGEM class as well as for our presentation at aGEM. | ||
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Revision as of 22:28, 20 August 2010
Friday August 20, 2010
Emily
Today I PCR purified malE and malE31. I then set up a digest of these, plus the psB1AK3 with a combination of enzymes: both EcoRI/SpeI and XbaI/PstI. This is to try to get the linearized PCR product into the AK BBK Construction plasmid. I then transformed these into TOP10 cells and plated them on AK plates. Today we also had a meeting in order to finalize some details for the iGEM class as well as for our presentation at aGEM.
No notebook page exists for this date. Sorry!
