Team:Calgary/14 June 2010

From 2010.igem.org

Monday June 14, 2010

Dev, Chris, and Jeremy: Today, we did the constructions of J13002 (a combination of a constitutive promoter with a RBS) and E0040 (GFP) and E0032 (YFP) to determine if the two fluorescent proteins were functional. As well, a plasmid switch was begun with the part J23032 into a Kan plasmid backbone so a selection pressure would be present when it was constructed with R0040. This construction was done using standard Biobrick cloning and then transformation into Top10 Competent cells. We received the parts from the registry in the form of agar stabs on friday June 11, 2010 but they were received too late to work with them. We also did a construction of B0015 to R0040 to begin the RNA detection circuit. The agar stabs were streaked onto plates and left to grow overnight of the parts I0500, K239000, K135000, and K274210.