Team:Calgary/11 June 2010

From 2010.igem.org

Dev, Jeremy, and Chris: Today, we spent most of the day preparing tubes of competent cells which could be used in future transformations from an original stock of Top10. As well, the overnight cultures of the plasmid transfers of E0032 and E0040 were Miniprepped using GenElute procedure so the plasmids could be constructed on Monday. The construction was not grown over the weekend so as not to overgrow the plates. In the afternoon, we had a group meeting with Dr. Cairine Logan (Our teacher sponsor) and Deirdre Lobb (Our laboratory technician) where we discussed the various aspects of the project, gave reports on what we had accomplished so far, diagrammed the circuits so we knew exactly what was going on, and made plans for the following week. One idea that came up during the meeting was the use of a Transcriptional Terminator to be the negative control so the systems would not be expressed. Paul (One of our advisors) presented a possible modelling project that we could incorporate using a Neural Network system in MatLab. More information to follow on this later.