Team:BIOTEC Dresden/Protocols:Cocultivation assay

From 2010.igem.org

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<li>Repeat measurements every <span class="markup time">5min for 3 hours</span></li>
<li>Repeat measurements every <span class="markup time">5min for 3 hours</span></li>
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<a rel="lightbox" class="border" style="width:300px; margin-left:20%"><img src="https://static.igem.org/mediawiki/2010/1/17/BiotecDresden_Pipetting_schema_cocultivation_l.png"></a>
<h3>Data processing</h3>
<h3>Data processing</h3>
<ul>
<ul>

Revision as of 02:49, 28 October 2010

Set-up

Tecan plate reader

  • Start the machine 20 minutes in advance and heat the chamber to 37°C. Put in the plate and start the measurement with the following setting:
  • Measure OD at 612 nm
  • Measure fluorescence with an excitation wavelength of 485nm and an emission wavelength of 535nm for both GFP and YFP
  • Shake
  • Repeat measurements every 5min for 3 hours

Data processing

  • Normalize the data by dividing all fluorescence data by the corresponding optical density
  • Subtract the obtained value of the reference column from the calculated relative fluorescence of the wells containing HHL
  • Plot the fluorescence data over the time
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