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From 2010.igem.org

Building Parts

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To create parts that we want to use in our finished kit, we first started by finding a standard plasmid in which we could put each part in to amplify it.

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} May 10, 2010 PCR amplified a KanR cassette from p1003 with primers that contained either the A and B' ends or the A' and B ends. Image of Gel