Team:Alberta/Building Parts
From 2010.igem.org
Building Parts
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10-05-2010
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.
Recipe:
- 1uL p1003
- 2.5uL prA_p1003+
- 2.5uL prB'_p1003-
- 5ul 10X PCR buffer
- 1ul 10uM dNTPs
- 2ul 50uM MgCl~2~
- 0.5uL Taq polymerase
- MilliQ H~2~O