Team:Alberta/Building Parts
From 2010.igem.org
(Difference between revisions)
Line 16: | Line 16: | ||
Recipe: | Recipe: | ||
- | :1uL p1003 | + | :1uL p1003 (approx. 1ng) |
:2.5uL prA_p1003+ | :2.5uL prA_p1003+ | ||
:2.5uL prB'_p1003- | :2.5uL prB'_p1003- | ||
- | : | + | :5uL 10X PCR buffer |
- | : | + | :1uL 10uM dNTPs |
- | : | + | :2uL 50uM MgCl~2~ |
:0.5uL Taq polymerase | :0.5uL Taq polymerase | ||
- | :MilliQ H~2~O | + | :35.5uL MilliQ H~2~O |
- | <!--Image of gel performed that day --> | + | Program: |
+ | |||
+ | |||
+ | <!--Image of gel performed that day. 5uL of each PCR reaction, 1uL of 10X loading dye and 4uL MilliQ water in a 1% agarose gel --> | ||
+ | |||
+ | ''' |
Revision as of 20:11, 31 May 2010
Building Parts
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10-05-2010
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.
Recipe:
- 1uL p1003 (approx. 1ng)
- 2.5uL prA_p1003+
- 2.5uL prB'_p1003-
- 5uL 10X PCR buffer
- 1uL 10uM dNTPs
- 2uL 50uM MgCl~2~
- 0.5uL Taq polymerase
- 35.5uL MilliQ H~2~O
Program: