Team:Alberta/Building Parts
From 2010.igem.org
(Difference between revisions)
Line 15: | Line 15: | ||
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends. | PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends. | ||
- | Recipe: :1uL p1003 | + | Recipe: |
+ | :1uL p1003 | ||
:2.5uL prA_p1003+ | :2.5uL prA_p1003+ | ||
:2.5uL prB'_p1003- | :2.5uL prB'_p1003- | ||
Line 24: | Line 25: | ||
:MilliQ H~2~O | :MilliQ H~2~O | ||
- | <-- | + | <!--Image of gel performed that day --> |
Revision as of 19:59, 31 May 2010
Building Parts
|
|
|
|
|
---|
10-05-2010
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.
Recipe:
- 1uL p1003
- 2.5uL prA_p1003+
- 2.5uL prB'_p1003-
- 5ul 10X PCR buffer
- 1ul 10uM dNTPs
- 2ul 50uM MgCl~2~
- 0.5uL Taq polymerase
- MilliQ H~2~O