Team:Alberta/Building Parts
From 2010.igem.org
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+ | '''10-05-2010''' | ||
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+ | PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends. | ||
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+ | Recipe: :1uL p1003 | ||
+ | :2.5uL prA_p1003+ | ||
+ | :2.5uL prB'_p1003- | ||
+ | :5ul 10X PCR buffer | ||
+ | :1ul 10uM dNTPs | ||
+ | :2ul 50uM MgCl~2~ | ||
+ | :0.5uL Taq polymerase | ||
+ | :MilliQ H~2~O | ||
+ | |||
+ | <--!Image of gel performed that day --> |
Revision as of 19:55, 31 May 2010
Building Parts
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10-05-2010
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.
Recipe: :1uL p1003
- 2.5uL prA_p1003+
- 2.5uL prB'_p1003-
- 5ul 10X PCR buffer
- 1ul 10uM dNTPs
- 2ul 50uM MgCl~2~
- 0.5uL Taq polymerase
- MilliQ H~2~O
<--!Image of gel performed that day -->