Team:Alberta/Building Parts

From 2010.igem.org

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'''10-05-2010'''
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PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.
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Recipe: :1uL p1003
 +
:2.5uL prA_p1003+
 +
:2.5uL prB'_p1003-
 +
:5ul 10X PCR buffer
 +
:1ul 10uM dNTPs
 +
:2ul 50uM MgCl~2~
 +
:0.5uL Taq polymerase
 +
:MilliQ H~2~O
 +
 +
<--!Image of gel performed that day -->

Revision as of 19:55, 31 May 2010

Building Parts


May
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

10-05-2010

PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.

Recipe: :1uL p1003

2.5uL prA_p1003+
2.5uL prB'_p1003-
5ul 10X PCR buffer
1ul 10uM dNTPs
2ul 50uM MgCl~2~
0.5uL Taq polymerase
MilliQ H~2~O

<--!Image of gel performed that day -->