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Team:Alberta/Building Parts
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== Building Parts == | == Building Parts == | ||
| - | + | To create parts that we want to use in our finished kit, we first started by finding a standard plasmid in which we could put each part in to amplify it. | |
Date | Date | ||
May 10, 2010 | May 10, 2010 | ||
PCR amplified a KanR cassette from p1003 with primers that contained either the A and B' ends or the A' and B ends. | PCR amplified a KanR cassette from p1003 with primers that contained either the A and B' ends or the A' and B ends. | ||
Image of Gel | Image of Gel | ||
Revision as of 15:21, 31 May 2010
Building Parts
To create parts that we want to use in our finished kit, we first started by finding a standard plasmid in which we could put each part in to amplify it. Date May 10, 2010 PCR amplified a KanR cassette from p1003 with primers that contained either the A and B' ends or the A' and B ends. Image of Gel
