Team:Alberta/Building Parts
From 2010.igem.org
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PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends. | PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends. |
Revision as of 20:22, 31 May 2010
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10-05-2010
PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.
Recipe:
- 1uL p1003 (approx. 1ng)
- 2.5uL prA_p1003+
- 2.5uL prB'_p1003-
- 5uL 10X PCR buffer
- 1uL 10uM dNTPs
- 2uL 50uM MgCl~2~
- 0.5uL Taq polymerase
- 35.5uL MilliQ H~2~O
Program: