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Team:Aberdeen Scotland/Protocols
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| + | <h2> Protocols</h2> | ||
| + | <p> | ||
| + | For our iGEM2010 project, we have used a variety of techniques such as - | ||
| + | <br><br> | ||
| + | |||
| + | Restriction digest of DNA plasmids | ||
| + | <br> | ||
| + | Gel electropheresis of DNA products to check for correct length and quantity of DNA present | ||
| + | <br> | ||
| + | Selective culture of E-coli and Yeast in liquid and Agar Medium (LB and SD) | ||
| + | <br> | ||
| + | PCR Amplification | ||
| + | <br> | ||
| + | Transformation of shuttle vectors into yeast | ||
| + | <br> | ||
| + | DNA extraction for E-coli by Spin Mini-Prep | ||
| + | <br> | ||
| + | Microscopy, Fluorimetry and FACS | ||
| + | </p> | ||
| + | |||
</html> | </html> | ||
Revision as of 13:50, 17 October 2010
University of Aberdeen - ayeSwitch
iGEM 2010
Protocols
For our iGEM2010 project, we have used a variety of techniques such as -
Restriction digest of DNA plasmids
Gel electropheresis of DNA products to check for correct length and quantity of DNA present
Selective culture of E-coli and Yeast in liquid and Agar Medium (LB and SD)
PCR Amplification
Transformation of shuttle vectors into yeast
DNA extraction for E-coli by Spin Mini-Prep
Microscopy, Fluorimetry and FACS
