http://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&feed=atom&action=historyTeam:Aberdeen Scotland/Constructs - Revision history2024-03-28T17:30:20ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=177826&oldid=prevSlam at 09:34, 27 October 20102010-10-27T09:34:09Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b>Description:</b> This was a construct already avalilable in the host laboratory, in which the MS2 RNA-binding protein was placed under the control of an inducible <i>MET17</i> promoter. This promoter is induced in the absence of methionine in the growth medium, and repressed by its presence.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b>Description:</b> This was a construct already avalilable in the host laboratory, in which the MS2 RNA-binding protein was placed under the control of an inducible <i>MET17</i> promoter. This promoter is induced in the absence of methionine in the growth medium, and repressed by its presence.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><b>Main use;</b> The MS2 RNA binding protein binds MS2 RNA stem loops, such as those present in the GAL1p-[Npep-GFP] construct (see 'Switch components' above). Thus co-transforming GAL1p-[Npep-GFP] with MET17p - [MS2] in yeast would allow us to verify that MS2 protein binding to MS2 RNA stem loops would inhibit expression of N-pep-GFP at the translational level.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><b>Main use;</b> The MS2 RNA binding protein binds MS2 RNA stem loops, such as those present in the GAL1p-[Npep-GFP] construct (see 'Switch components' above). Thus co-transforming GAL1p-[Npep-GFP] with MET17p - [MS2] in yeast would allow us to verify that MS2 protein binding to MS2 RNA stem loops would inhibit expression of N-pep-GFP at the translational level.<ins class="diffchange diffchange-inline"><a href="https://2010.igem.org/MS2_Coat-Protein_Effect_on_Expression_of_GFP_in_pRS415"><i>See results</i></a></ins></p></div></td></tr>
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</table>Slamhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=158606&oldid=prevPorter at 12:05, 26 October 20102010-10-26T12:05:07Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results">Continue to Results&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/3/36/Right_arrow.png"></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results">Continue to Results <ins class="diffchange diffchange-inline">Main Page</ins>&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2010/3/36/Right_arrow.png"></a></div></td></tr>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=138046&oldid=prevPorter at 22:05, 24 October 20102010-10-24T22:05:52Z<p></p>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=135100&oldid=prevPorter at 17:31, 24 October 20102010-10-24T17:31:38Z<p></p>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=134181&oldid=prevPorter at 16:29, 24 October 20102010-10-24T16:29:11Z<p></p>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=133739&oldid=prevPorter at 15:56, 24 October 20102010-10-24T15:56:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><i>For each construct, we provide a brief description, and its intended use;</i></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><i>For each construct, we provide a brief description, and its intended use;</i></b></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>1. Promoter characterisation constructs</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>1. Promoter characterisation constructs</h2></div></td></tr>
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</table>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=133730&oldid=prevPorter at 15:55, 24 October 20102010-10-24T15:55:36Z<p></p>
<a href="http://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=133730&oldid=106529">Show changes</a>Porterhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=106529&oldid=prevI.stansfield at 15:51, 17 October 20102010-10-17T15:51:32Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b>Main use;</b> for troubleshooting experiments. This construct was made by the team to allow us to verify that our fluorescent microscopes and cell cytometer (FACS) was able to successfully detect CFP expressed in yeast. It was used to confirm whether or not the CFP sequence from CUP1p - [MS2-CFP] <del class="diffchange diffchange-inline">was </del>able to exhibit CFP fluorescence. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See 'Results/Troubleshooting'</i></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>Main use;</b> for troubleshooting experiments. This construct was made by the team to allow us to verify that our fluorescent microscopes and cell cytometer (FACS) was able to successfully detect CFP expressed in yeast. It was used to confirm whether or not the CFP sequence from CUP1p - [MS2-CFP] <ins class="diffchange diffchange-inline">were </ins>able to exhibit CFP fluorescence. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See 'Results/Troubleshooting'</i></a></div></td></tr>
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</table>I.stansfieldhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=106093&oldid=prevI.stansfield at 10:51, 17 October 20102010-10-17T10:51:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><h4>GAL1p-[GFP]</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><h4>GAL1p-[GFP]</h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Description:</b> This is a genomically-integrated construct in which the GFP protein was placed under control of the yeast GAL1 promoter (Fig. 1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Description:</b> This is a genomically-integrated construct in which the GFP protein was placed under control of the yeast GAL1 promoter (Fig. 1)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Main use;</b> to characterise the induction characteristics of the <i>GAL1</i> promoter, which is induced by galactose. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See promoter activity assay results</i></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Main use;</b> to characterise the induction characteristics of the <i>GAL1</i> promoter, which is induced by galactose. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See promoter activity assay results</i></a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><h4>CUP1p-[GFP]</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><h4>CUP1p-[GFP]</h4></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Description:</b> This is a genomically integrated construct in which the GFP protein was placed under control of the yeast CUP1 promoter </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Description:</b> This is a genomically integrated construct in which the GFP protein was placed under control of the yeast CUP1 promoter </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Main use;</b> to characterise the induction characteristics of the <i>CUP1</i> promoter, which is induced by copper ions. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See promoter activity assay results</i></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Main use;</b> to characterise the induction characteristics of the <i>CUP1</i> promoter, which is induced by copper ions. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See promoter activity assay results</i></a></div></td></tr>
</table>I.stansfieldhttp://2010.igem.org/wiki/index.php?title=Team:Aberdeen_Scotland/Constructs&diff=105907&oldid=prevI.stansfield at 06:52, 17 October 20102010-10-17T06:52:54Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Main use;</b> for troubleshooting experiments. This construct was made by the team to allow us to verify that our fluorescent microscopes and cell cytometer (FACS) was able to successfully detect CFP expressed in yeast. It was used to confirm whether or not the CFP sequence from CUP1p - [MS2-CFP] was able to exhibit CFP fluorescence. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See 'Results/Troubleshooting'</i></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Main use;</b> for troubleshooting experiments. This construct was made by the team to allow us to verify that our fluorescent microscopes and cell cytometer (FACS) was able to successfully detect CFP expressed in yeast. It was used to confirm whether or not the CFP sequence from CUP1p - [MS2-CFP] was able to exhibit CFP fluorescence. <a href="https://2010.igem.org/Team:Aberdeen_Scotland/Results"><i>See 'Results/Troubleshooting'</i></a></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2010/c/c7/TEF1_promoter.jpg"/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2010/c/c7/TEF1_promoter.jpg"/<ins class="diffchange diffchange-inline">></center</ins>></div></td></tr>
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</table>I.stansfield