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From 2010.igem.org

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Restriction Enzyme digestion —One by one

Team

Project -Sudoku --Abstract --construct --Lab note ---Parts Code ---Notebook:Main construct ---Notebook:Terminator leak switch ---Notebook:flpe ---Notebook:Virus expression ---Notebook:Anti-RNA repression --Result

-Allergy --Abstract --construct --Lab note

-Bioconcentration --Abstract --construct --Lab note

Parts -Check

Protocols -Transformation -Colony Pick Up -Miniprep -Restriction Enzyme Transaction（Xba1/Pst1）?Once for all -Restriction Enzyme Transaction ?One by one -PCR（Pfu　Ultra） -PCR（Ex-Taq） -PCR（KODplus） -Colony PCR（Ex-Taq） -Electrophoresis -Gel DNA Recovery -LB　broth -Agarose Gel -Sequencing -Producing Competent Cell -phosphorylation -phosphorylation & ligatibn

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