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http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-28T03:57:55Z
<p>Hotpotato: /* 2. Pulse to GFP generator */</p>
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[[Team:Chiba/System 1|<<Back]]<br />
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<font size=5>Version 1 :Testing individual parts</font><br />
<br />
To realize the genetic double click system, we made two plasmids. First one is ''Pulse generator'', which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The second one is ''pulse -> GFP generator'', which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. <br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
==1. Pulse generator==<br />
For Pulse generator, the parts shown below were checked.Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
===T7/CI-OR1 hybrid promoter===<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
====Experiment&Results====<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
*Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. Journal of Molecular Biology 219, 45-59 (1991).<br />
<br />
<br />
==2. Pulse to GFP generator==<br />
For GFP generator, the parts shown below were checked.<br />
<br />
===Lux/CI434 hybrid promoter===<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
====Rationale====<br />
[[image:Hybrid.png]]<br />
<br><br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation.<br />
<br />
====Characterization of lux/CI434-OR1&OR2 hybrid promoter(BBa_K396001)====<br />
lux/CI434-OR1&OR2 hybrid promoter was designed to be activated by LuxR-AHL dimmer and repressed by 434 phage CI434 protein. This part was experimented whether GFP downstream of the hybrid was induced by AHL. Promoter.<br />
[[Image:그림1.png|600px|thumb|center|alt text]]<br />
<br><br />
[[Image:그림2.png|600px|thumb|center|alt text]]<br />
<br><br />
[[Image:그림3.png|600px|thumb|center|alt text]]<br />
====Ref====<br />
#http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
#http://partsregistry.org/Part:BBa_I12040<br />
#Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101: 6355-6360.</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-28T03:56:29Z
<p>Hotpotato: /* 2. Pulse to GFP generator */</p>
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[[Team:Chiba/System 1|<<Back]]<br />
<br><br><br />
<font size=5>Version 1 :Testing individual parts</font><br />
<br />
To realize the genetic double click system, we made two plasmids. First one is ''Pulse generator'', which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The second one is ''pulse -> GFP generator'', which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. <br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
==1. Pulse generator==<br />
For Pulse generator, the parts shown below were checked.Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
===T7/CI-OR1 hybrid promoter===<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
====Experiment&Results====<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
*Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. Journal of Molecular Biology 219, 45-59 (1991).<br />
<br />
<br />
==2. Pulse to GFP generator==<br />
For GFP generator, the parts shown below were checked.<br />
<br />
===Lux/CI434 hybrid promoter===<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
====Rationale====<br />
[[image:Hybrid.png]]<br />
<br><br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation.<br />
<br />
====Characterization of lux/CI434-OR1&OR2 hybrid promoter(BBa_K396001)====<br />
lux/CI434-OR1&OR2 hybrid promoter was designed to be activated by LuxR-AHL dimmer and repressed by 434 phage CI434 protein. This part was experimented whether GFP downstream of the hybrid was induced by AHL. Promoter.<br />
<center><br />
[[Image:그림1.png|600px|thumb|left|alt text]]<br />
<br><br />
[[Image:그림2.png|600px|thumb|left|alt text]]<br />
<br><br />
[[Image:그림3.png|600px|thumb|left|alt text]]<br />
</center><br />
====Ref====<br />
#http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
#http://partsregistry.org/Part:BBa_I12040<br />
#Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101: 6355-6360.</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-28T03:55:38Z
<p>Hotpotato: /* 2. Pulse to GFP generator */</p>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
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<!-- -------------------------------------------------------------------------------<br />
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__NOTOC__<br />
<br><br><br />
[[Team:Chiba/System 1|<<Back]]<br />
<br><br><br />
<font size=5>Version 1 :Testing individual parts</font><br />
<br />
To realize the genetic double click system, we made two plasmids. First one is ''Pulse generator'', which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The second one is ''pulse -> GFP generator'', which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. <br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
==1. Pulse generator==<br />
For Pulse generator, the parts shown below were checked.Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
===T7/CI-OR1 hybrid promoter===<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
====Experiment&Results====<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
*Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. Journal of Molecular Biology 219, 45-59 (1991).<br />
<br />
<br />
==2. Pulse to GFP generator==<br />
For GFP generator, the parts shown below were checked.<br />
<br />
===Lux/CI434 hybrid promoter===<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
====Rationale====<br />
[[image:Hybrid.png]]<br />
<br><br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation.<br />
<br />
====Characterization of lux/CI434-OR1&OR2 hybrid promoter(BBa_K396001)====<br />
lux/CI434-OR1&OR2 hybrid promoter was designed to be activated by LuxR-AHL dimmer and repressed by 434 phage CI434 protein. This part was experimented whether GFP downstream of the hybrid was induced by AHL. Promoter.<br />
[[Image:그림1.png|600px|thumb|left|alt text]]<br />
<br><br />
[[Image:그림2.png|600px|thumb|left|alt text]]<br />
<br><br />
[[Image:그림3.png|600px|thumb|left|alt text]]<br />
<br />
====Ref====<br />
#http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
#http://partsregistry.org/Part:BBa_I12040<br />
#Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101: 6355-6360.</div>
Hotpotato
http://2010.igem.org/File:%EA%B7%B8%EB%A6%BC3.png
File:그림3.png
2010-10-28T03:53:24Z
<p>Hotpotato: </p>
<hr />
<div></div>
Hotpotato
http://2010.igem.org/File:%EA%B7%B8%EB%A6%BC2.png
File:그림2.png
2010-10-28T03:52:47Z
<p>Hotpotato: </p>
<hr />
<div></div>
Hotpotato
http://2010.igem.org/File:%EA%B7%B8%EB%A6%BC1.png
File:그림1.png
2010-10-28T03:50:25Z
<p>Hotpotato: </p>
<hr />
<div></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-28T03:49:33Z
<p>Hotpotato: /* 2. Pulse to GFP generator */</p>
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<br><br><br />
[[Team:Chiba/System 1|<<Back]]<br />
<br><br><br />
<font size=5>Version 1 :Testing individual parts</font><br />
<br />
To realize the genetic double click system, we made two plasmids. First one is ''Pulse generator'', which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The second one is ''pulse -> GFP generator'', which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. <br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
==1. Pulse generator==<br />
For Pulse generator, the parts shown below were checked.Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
===T7/CI-OR1 hybrid promoter===<br />
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396000 BBa_K396000]<br />
T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed.<br />
====Experiment&Results====<br />
[[Image:CI-1.jpg|frame|center|Fig. How to check the parts]]<br />
[[Image:CI-2.jpg|frame|center|Fig. Data: Under the situation of CI non-expressed , T7 RNAP causes GFP expression by activating T7/CI hybrid promoter (Fig.1). On the other hand, under the situation of CI expressed, T7 RNAP does not cause GFP expression (Fig.2). The reason of this is considered as following. Under the situation of CI expressed, even though T7 RNAP tries to activate T7/CI promoter, the promoter Is repressed by CI because the ability of CI repression is superior to the one of T7 activation for this promoter. So it is concluded that T7/CI hybrid promoter is to be activated by T7 RNA Polymerase and repressed by lambda CI protein, following the rules of CI repression > T7 activation.]]<br />
*Dubendorf, J.W. & Studier, F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. Journal of Molecular Biology 219, 45-59 (1991).<br />
<br />
<br />
==2. Pulse to GFP generator==<br />
For GFP generator, the parts shown below were checked.<br />
<br />
===Lux/CI434 hybrid promoter===<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
====Rationale====<br />
[[image:Hybrid.png]]<br />
<br><br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) at downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation.<br />
<br />
====Characterization of lux/CI434-OR1&OR2 hybrid promoter(BBa_K396001)====<br />
lux/CI434-OR1&OR2 hybrid promoter was designed to be activated by LuxR-AHL dimmer and repressed by 434 phage CI434 protein. This part was experimented whether GFP downstream of the hybrid was induced by AHL. Promoter.<br />
<br />
<br />
====Ref====<br />
#http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
#http://partsregistry.org/Part:BBa_I12040<br />
#Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101: 6355-6360.</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2/Result
Team:Chiba/System 2/Result
2010-10-28T03:37:25Z
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[[Team:Chiba/System 2|<<Back]]<br><br><br />
<font size=6>Version 2 :</font><br />
===<font size="5">Abstract</font>===<br />
-----<br />
LuxR is AHL-dependent activator. LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription. However lux box is inserted between -35 and -10, LuxR functions as AHL-dependent inverter (Plux inv). Plux inv was resistered in Biobrick number R0061. We've prepared Plux inv-GFP and characterized about it.<br><br><br><br />
<br />
===<font size="5">Experiments</font>===<br />
-----<br />
<br />
===1.Construction of Plux inv-GFP===<br />
<br />
Construction process is shown in '''Fig. 1'''.We constructed Plux inv-GFP conbining R0061 and E0240 and transformed by strain of XL10-G.<br />
<br />
GFP fluorescence and sequence is confirmed. <br />
<br />
[[Image:Plux inv-GFP.png|none|frame|right|'''Fig. 1''' Evaluation of LuxR Inverter]]<br />
<br />
===2.Characterization Plux inv-GFP===<br />
<br />
strain:DH10B<br><br />
sample<br><br />
1,Plux inv-GFP and Plac-LuxR(PSB1A3) <br><br />
2,Plux inv-GFP (Positive control)<br><br />
3,Plux inv (Negative control)<br><br />
add AHL1000 nM (AHL+)and<br />
<br />
Each sample is incubated for 12 h at 37゜C <br />
After incubation,Spin-dawn(14500rpm,1min) and observed the pellet in the UV.<br />
[[Image:Plux inv function.png|none|frame|Fig. 2 characterization of Plux inv-GFP]]<br />
<br><br><br><br />
<br />
===<font size="5">Results</font>===<br />
-----<br />
Pellet Photos is shown in '''Fig. 3'''.We confirmed GFP fluorescence both AHL+ and AHL-.So We're not able to confirm LuxR repression. <br />
<br />
[[Image:Plux inv results.png|none|right|frame|Fig. 3 Pellet photos of the incubeted sample]]<br />
<br />
===Conclusion===<br />
<br />
We're not able to confirm LuxR repression.<br><br />
However,''iGEMTokyo_tech2010'' team also charcterized about '''Plux inv''' and succeeded in repressing transcription .<br><br />
<br />
It does not completely repress transcription, Plasmids, strains ,culture conditions,and detection method LuxR suppression can not be confirmed(Cox et al,2007).<br><br />
<br><br />
<br />
===<font size="5">Reference</font>===<br />
-----<br />
#Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)<br />
#Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2
Team:Chiba/System 2
2010-10-28T03:34:58Z
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
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<!-------- Main --------><br />
=Version 2=<br />
<br />
==Overview==<br />
{|-<br />
|Our second circiut of double click system is called <b>Recognize circuit</b>.<br><br />
It consists of <b>recognizing</b> system and <b>memorizing</b> system.<br><br />
Recognizing system can recognize the existence of input.<br><br />
And Memorizing system can memorize the existence of input in the past for a while.<br><br />
When the two system work together, bacteria is going to shine.<br><br />
We hope this circuit works as double click system.<br />
|[[Image:Chiba_cI.jpg]]<br />
|}<br />
<br />
We use AHL for input and regard injection of AHL as clicking. In our project, CLICK controls transcription of overall DNA sequence. <br><br />
In recognizing input system, there is a cI operator above gfp DNA sequence. cI recognizes the existence of input in this DNA sequence.<br><br />
In memorizing system, there is an AND Gate with T7ptag and supD. In this case, the AND Gate remembers that there was a click.<br><br />
There is a hybrid promoter(cI/T7) above gfp DNA sequence. It is regulated by T7 and cI which work as activator and repressor, respectively. On this promoter, repression is stronger than activation. And also, it is low unregulated activation. Working of this promoter depends on recognizing and memorizing system.<br><br />
[[Team:Chiba/System_2/Overall|details...]]<br />
<br><br><br><br />
<br />
==Circuit Construction==<br />
We've designed DNA sequence like following Figure. <br><br />
<center><br />
[[Image:Chiba_Sys2.jpg]]</center><br />
Based on this sequence, we've also designed vector.<br><br />
<center><br />
[[Image:Chiba_ver2.jpg]]</center><br />
Prom-luxR&PT7/cI-GFP vector is constructed collaboration with Double Click System Version 1.<br><br><br><br />
<br />
==Result==<br />
<br />
===Testing===<br />
In this system, lux promoter inverter is sensor of input.<br />
We've researched Plux inverter and got some results.<br><br />
We need lux promoter repressed by AHL-luxR dimer. But Plux inverter of biobrick didn't work. So we've tried to charaterize it and make Plux inverter.<br><br />
LuxR is AHL-dependent activator. LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription. However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061. We've prepared Plux inv-GFP and characterized about it. [[Team:Chiba/System_2/Result|details...]]<br />
<br />
===Evaluation===<br />
<br><br />
===Remark&Notes===<br />
<br><br />
<br />
==Conclusion==<br />
It does not completely repress transcription, Plasmids, strains ,culture conditions,and detection method LuxR suppression can not be confirmed(Cox et al,2007).<br><br />
[[Team:Chiba/System_2/Result|details...]]<br />
<br><br><br></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2
Team:Chiba/System 2
2010-10-28T03:30:28Z
<p>Hotpotato: /* Circuit Construction */</p>
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<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
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<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<!-------- Main --------><br />
=Version 2=<br />
<br />
==Overview==<br />
{|-<br />
|Our second circiut of double click system is called <b>Recognize circuit</b>.<br><br />
It consists of <b>recognizing</b> system and <b>memorizing</b> system.<br><br />
Recognizing system can recognize the existence of input.<br><br />
And Memorizing system can memorize the existence of input in the past for a while.<br><br />
When the two system work together, bacteria is going to shine.<br><br />
We hope this circuit works as double click system.<br />
|[[Image:Chiba_cI.jpg]]<br />
|}<br />
<br />
We use AHL for input and regard injection of AHL as clicking. In our project, CLICK controls transcription of overall DNA sequence. <br><br />
In recognizing input system, there is a cI operator above gfp DNA sequence. cI recognizes the existence of input in this DNA sequence.<br><br />
In memorizing system, there is an AND Gate with T7ptag and supD. In this case, the AND Gate remembers that there was a click.<br><br />
There is a hybrid promoter(cI/T7) above gfp DNA sequence. It is regulated by T7 and cI which work as activator and repressor, respectively. On this promoter, repression is stronger than activation. And also, it is low unregulated activation. Working of this promoter depends on recognizing and memorizing system.<br><br />
[[Team:Chiba/System_2/Overall|details...]]<br />
<br><br><br><br />
<br />
==Circuit Construction==<br />
We've designed DNA sequence like following Figure. <br><br />
<center><br />
[[Image:Chiba_Sys2.jpg]]</center><br />
Based on this sequence, we've also designed vector.<br><br />
<center><br />
[[Image:Chiba_ver2.jpg]]</center><br />
Prom-luxR&PT7/cI-GFP vector is constructed collaboration with Double Click System Version 1.<br><br><br><br />
<br />
==Result==<br />
<br />
===Testing===<br />
In this system, lux promoter inverter is sensor of input.<br />
We've researched Plux inverter and got some results.<br><br />
We need lux promoter repressed by AHL-luxR dimer. But Plux inverter of biobrick didn't work. So we've tried to charaterize it and make Plux inverter.<br><br />
LuxR is AHL-dependent activator.LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription.However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061.We've prepared Plux inv-GFP and characterized about it. [[Team:Chiba/System_2/Result|details...]]<br />
<br />
===Evaluation===<br />
<br><br />
===Remark&Notes===<br />
<br><br />
<br />
==Conclusion==<br />
It does not completely repress transcription, Plasmids, strains ,culture conditions,and detection method LuxR suppression can not be confirmed(Cox et al,2007).<br><br />
[[Team:Chiba/System_2/Result|details...]]<br />
<br><br><br></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2
Team:Chiba/System 2
2010-10-28T03:29:17Z
<p>Hotpotato: /* Circuit Construction */</p>
<hr />
<div><!-- ---------------------------------------------------------<br />
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下の方に「ここから下を編集」というところがあります<br />
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#tabs10 {<br />
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line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
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#tabs10 a:hover {<br />
background-position:0% -42px;<br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
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<!-- -------------------------------------------------------------------------------<br />
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<!-------- Main --------><br />
=Version 2=<br />
<br />
==Overview==<br />
{|-<br />
|Our second circiut of double click system is called <b>Recognize circuit</b>.<br><br />
It consists of <b>recognizing</b> system and <b>memorizing</b> system.<br><br />
Recognizing system can recognize the existence of input.<br><br />
And Memorizing system can memorize the existence of input in the past for a while.<br><br />
When the two system work together, bacteria is going to shine.<br><br />
We hope this circuit works as double click system.<br />
|[[Image:Chiba_cI.jpg]]<br />
|}<br />
<br />
We use AHL for input and regard injection of AHL as clicking. In our project, CLICK controls transcription of overall DNA sequence. <br><br />
In recognizing input system, there is a cI operator above gfp DNA sequence. cI recognizes the existence of input in this DNA sequence.<br><br />
In memorizing system, there is an AND Gate with T7ptag and supD. In this case, the AND Gate remembers that there was a click.<br><br />
There is a hybrid promoter(cI/T7) above gfp DNA sequence. It is regulated by T7 and cI which work as activator and repressor, respectively. On this promoter, repression is stronger than activation. And also, it is low unregulated activation. Working of this promoter depends on recognizing and memorizing system.<br><br />
[[Team:Chiba/System_2/Overall|details...]]<br />
<br><br><br><br />
<br />
==Circuit Construction==<br />
We've designed DNA sequence like following Figure. <br><br />
<center><br />
[[Image:Chiba_Sys2.jpg]]</center><br />
Based on this sequence, we've also designed vector.<br><br />
<center><br />
[[Image:Chiba_ver2.jpg]]</center><br />
Prom-luxR&PT7/cI-GFP vector is constructed collaboration with double click system version 1.<br><br><br><br />
<br />
==Result==<br />
<br />
===Testing===<br />
In this system, lux promoter inverter is sensor of input.<br />
We've researched Plux inverter and got some results.<br><br />
We need lux promoter repressed by AHL-luxR dimer. But Plux inverter of biobrick didn't work. So we've tried to charaterize it and make Plux inverter.<br><br />
LuxR is AHL-dependent activator.LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription.However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061.We've prepared Plux inv-GFP and characterized about it. [[Team:Chiba/System_2/Result|details...]]<br />
<br />
===Evaluation===<br />
<br><br />
===Remark&Notes===<br />
<br><br />
<br />
==Conclusion==<br />
It does not completely repress transcription, Plasmids, strains ,culture conditions,and detection method LuxR suppression can not be confirmed(Cox et al,2007).<br><br />
[[Team:Chiba/System_2/Result|details...]]<br />
<br><br><br></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2
Team:Chiba/System 2
2010-10-28T03:26:23Z
<p>Hotpotato: /* Overview */</p>
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display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
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#tabs10 a span {float:none;}<br />
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#tabs9 a:hover span {<br />
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#tabs10 a:hover {<br />
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background-position:100% -42px;<br />
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
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color:#FFF;<br />
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/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
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#tabs9 a:hover {<br />
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<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
</div><br />
</body><br />
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<!--- 2nd tab End---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<!-------- Main --------><br />
=Version 2=<br />
<br />
==Overview==<br />
{|-<br />
|Our second circiut of double click system is called <b>Recognize circuit</b>.<br><br />
It consists of <b>recognizing</b> system and <b>memorizing</b> system.<br><br />
Recognizing system can recognize the existence of input.<br><br />
And Memorizing system can memorize the existence of input in the past for a while.<br><br />
When the two system work together, bacteria is going to shine.<br><br />
We hope this circuit works as double click system.<br />
|[[Image:Chiba_cI.jpg]]<br />
|}<br />
<br />
We use AHL for input and regard injection of AHL as clicking. In our project, CLICK controls transcription of overall DNA sequence. <br><br />
In recognizing input system, there is a cI operator above gfp DNA sequence. cI recognizes the existence of input in this DNA sequence.<br><br />
In memorizing system, there is an AND Gate with T7ptag and supD. In this case, the AND Gate remembers that there was a click.<br><br />
There is a hybrid promoter(cI/T7) above gfp DNA sequence. It is regulated by T7 and cI which work as activator and repressor, respectively. On this promoter, repression is stronger than activation. And also, it is low unregulated activation. Working of this promoter depends on recognizing and memorizing system.<br><br />
[[Team:Chiba/System_2/Overall|details...]]<br />
<br><br><br><br />
<br />
==Circuit Construction==<br />
We've designed DNA sequence like following Figure. <br><br />
<center><br />
[[Image:Chiba_Sys2.jpg]]</center><br />
Based on this sequence, we've also designed vertor.<br><br />
<center><br />
[[Image:Chiba_ver2.jpg]]</center><br />
Prom-luxR&PT7/cI-GFP vector is constructed collaboration with double click system version 1.<br><br><br><br />
==Result==<br />
<br />
===Testing===<br />
In this system, lux promoter inverter is sensor of input.<br />
We've researched Plux inverter and got some results.<br><br />
We need lux promoter repressed by AHL-luxR dimer. But Plux inverter of biobrick didn't work. So we've tried to charaterize it and make Plux inverter.<br><br />
LuxR is AHL-dependent activator.LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription.However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061.We've prepared Plux inv-GFP and characterized about it. [[Team:Chiba/System_2/Result|details...]]<br />
<br />
===Evaluation===<br />
<br><br />
===Remark&Notes===<br />
<br><br />
<br />
==Conclusion==<br />
It does not completely repress transcription, Plasmids, strains ,culture conditions,and detection method LuxR suppression can not be confirmed(Cox et al,2007).<br><br />
[[Team:Chiba/System_2/Result|details...]]<br />
<br><br><br></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2
Team:Chiba/System 2
2010-10-28T03:25:25Z
<p>Hotpotato: /* Overview */</p>
<hr />
<div><!-- ---------------------------------------------------------<br />
--------------------------------------------------------------<br />
下の方に「ここから下を編集」というところがあります<br />
そっちを編集してください<br />
--------------------------------------------------------------<br />
------------------------------------------------------------ --><br />
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
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font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
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img {<br />
border: none;<br />
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/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
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margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
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background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
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#tabs10 a span {float:none;}<br />
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#tabs9 a:hover span {<br />
background-color: #ff3366;<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
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#tabs10 a:hover span {<br />
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#tabs10 #current a {<br />
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background-position:100% -42px;<br />
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
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<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Double Click System</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>Version 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>Version 2</span></a></li> </ul><br />
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<!-- -------------------------------------------------------------------------------<br />
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<!-------- Main --------><br />
=Version 2=<br />
<br />
==Overview==<br />
{|-<br />
|Our second circiut of double click system is called <b>Recognize circuit</b>.<br><br />
It consists of <b>recognizing</b> system and <b>memorizing</b> system.<br><br />
Recognizing system can recognize the existence of input.<br><br />
And Memorizing system can memorize the existence of input in the past for a while.<br><br />
When the two system work together, bacteria is going to shine.<br><br />
We hope this circuit works as double click system.<br />
|[[Image:Chiba_cI.jpg]]<br />
|}<br />
<br />
We use AHL for input and regard injection of AHL as clicking. In our project, CLICK controls transcription of overall DNA sequence. <br><br />
In recognizing input system, there is a cI operator above gfp DNA sequence. cI recognizes the existence of input in this DNA sequence.<br><br />
In memorizing system, there is an AND Gate with T7ptag and supD. In this case, the AND Gate remembers that there was a click.<br><br />
There is a hybrid promoter(cI/T7) above gfp DNA sequence. It is regulated by T7 and cI which work as activator and repressor, respectively. On this promoter, repression is stronger than activation. And also, it is low unregulated activation. Working of this promoter depends on recognizing and memorizing system.<br><br />
[[Team:Chiba/System_2/Overall|detail...]]<br />
<br><br><br><br />
<br />
==Circuit Construction==<br />
We've designed DNA sequence like following Figure. <br><br />
<center><br />
[[Image:Chiba_Sys2.jpg]]</center><br />
Based on this sequence, we've also designed vertor.<br><br />
<center><br />
[[Image:Chiba_ver2.jpg]]</center><br />
Prom-luxR&PT7/cI-GFP vector is constructed collaboration with double click system version 1.<br><br><br><br />
==Result==<br />
<br />
===Testing===<br />
In this system, lux promoter inverter is sensor of input.<br />
We've researched Plux inverter and got some results.<br><br />
We need lux promoter repressed by AHL-luxR dimer. But Plux inverter of biobrick didn't work. So we've tried to charaterize it and make Plux inverter.<br><br />
LuxR is AHL-dependent activator.LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription.However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061.We've prepared Plux inv-GFP and characterized about it. [[Team:Chiba/System_2/Result|details...]]<br />
<br />
===Evaluation===<br />
<br><br />
===Remark&Notes===<br />
<br><br />
<br />
==Conclusion==<br />
It does not completely repress transcription, Plasmids, strains ,culture conditions,and detection method LuxR suppression can not be confirmed(Cox et al,2007).<br><br />
[[Team:Chiba/System_2/Result|details...]]<br />
<br><br><br></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-28T03:22:33Z
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<tr><br />
<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">Would any of your project ideas raise safety issues in terms of: </strong></font><br><br />
- Not at all. All our circuits are made of genetic regulators and reporters (fluorescent proteins).<br><br />
Our project goal -double click system- is designed to improve the safety in operating modified organisms.<br><br />
<br><br><br />
<strong><font size="2"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong></font><br><br />
- Our biobricks are composed of gene regulators, RNA polymerase, hybrid promoters, and fluorescent proteins only. None of them (and the combination of those parts/ devices) could raise the safety issue.<br />
<br />
<br />
Thus, we are working on the super-safe side of Synthetic Biology!<br><br />
</td><br />
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</body><br />
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<br />
<br />
[[Safety |Go to safety page]].</div>
Hotpotato
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-28T03:21:43Z
<p>Hotpotato: </p>
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<br />
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<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">Would any of your project ideas raise safety issues in terms of: </strong></font><br><br />
- Not at all. All our circuits are made of genetic regulators and reporters (fluorescent proteins).<br><br />
Our project goal -double click system- is designed to improve the safety in operating modified organisms.<br><br />
<br><br><br />
<strong><font size="2"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong></font><br><br />
- Our biobricks are composed of gene regulators, RNA polymerase, hybrid promoters, and fluorescent proteins only. None of them (and the combination of those parts/ devices) could raise the safety issue.<br />
<br />
<br />
Thus, we are working on the super-safe side of Synthetic Biology!<br><br />
<a href="http://igemjapan.net/">http://igemjapan.net/</a><br><br />
<a href="http://igemjapan.net/wiki/">http://igemjapan.net/wiki/</a><br />
</td><br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<br />
[[Safety |Go to safety page]].</div>
Hotpotato
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-28T03:21:00Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
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color: #000;<br />
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float:left;<br />
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line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
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#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
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#tabs10 a span {<br />
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display:block;<br />
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<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
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<!--- Background Contents End ---><br />
<!--- Main Contents Start ---><br />
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<body><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">Would any of your project ideas raise safety issues in terms of: </strong></font><br><br />
- Not at all. All our circuits are made of genetic regulators and reporters (fluorescent proteins).<br><br />
Our project goal -double click system- is designed to improve the safety in operating modified organisms.<br><br />
<br><br><br />
<strong><font size="2"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong></font><br><br />
- Our biobricks are composed of gene regulators, RNA polymerase, hybrid promoters, and fluorescent proteins only. None of them (and the combination of those parts/ devices) could raise the safety issue.<br />
<br />
<br />
Thus, we are working on the super-safe side of Synthetic Biology!<br />
<a href="http://igemjapan.net/">http://igemjapan.net/</a><br />
<a href="http://igemjapan.net/wiki/">http://igemjapan.net/wiki/</a><br />
</td><br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<br />
[[Safety |Go to safety page]].</div>
Hotpotato
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-27T23:26:50Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
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<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
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<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
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<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
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<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
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margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
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font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
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border: none;<br />
}<br />
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/*- Menu Tabs 10--------------------------- */<br />
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#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
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list-style:none;<br />
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#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
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<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- Background Contents End ---><br />
<!--- Main Contents Start ---><br />
<html><br />
<body><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">Would any of your project ideas raise safety issues in terms of: </strong></font><br><br />
- Our theme is double click system in bacteria and we expect that it will be used for eliminating false input.<br> <br />
It can raise accuracy of work to the target and reduce side effect by responding double click.<br><br />
We hope this system to be used for safety device on labs.<br><br><br />
<strong><font size="2"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong></font><br><br />
- We've desinged pulse generator and some hybrid promoters. We've also registed the parts.<br><br />
We think construction of these parts can be used as safety device.<br><br />
For example, hybrid promoter is low unregulated and repression for the promoter is stronger than activation for.<br><br />
So, expression of protein occurs in specific environment which can mean safety device.<br><br />
If reaction needs the protein expressed by pulse, the pulse also can be safety device.<br><br />
Because, reaction must be done in fixed time whose the protein exists in. <br><br><br />
<a href="http://igemjapan.net/"></a><br />
<a href="http://igemjapan.net/wiki/"></a><br />
</td><br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<br />
[[Safety |Go to safety page]].</div>
Hotpotato
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-27T23:24:25Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
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<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
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<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
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<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
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font: bold 11px/1.5em Verdana;<br />
}<br />
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font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
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border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
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display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
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/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
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<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- Background Contents End ---><br />
<!--- Main Contents Start ---><br />
<html><br />
<body><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">Would any of your project ideas raise safety issues in terms of: </strong></font><br><br />
- Our theme is double click system in bacteria and we expect that it will be used for eliminating false input.<br> <br />
It can raise accuracy of work to the target and reduce side effect by responding double click.<br><br />
We hope this system to be used for safety device on labs.<br><br><br />
<strong><font size="2"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong></font><br><br />
- We've desinged pulse generator and some hybrid promoters. We've also registed the parts.<br><br />
We think construction of these parts can be used as safety device.<br><br />
For example, hybrid promoter is low unregulated and repression for the promoter is stronger than activation for.<br><br />
So, expression of protein occurs in specific environment which can mean safety device.<br><br />
If reaction needs the protein expressed by pulse, the pulse also can be safety device.<br><br />
Because, reaction must be done in fixed time whose the protein exists in. <br><br><br />
</td><br />
</tr><br />
http://igemjapan.net/<br />
http://igemjapan.net/wiki/<br />
</table><br />
</body><br />
</html><br />
<br />
<br />
[[Safety |Go to safety page]].</div>
Hotpotato
http://2010.igem.org/Team:Chiba/Safety
Team:Chiba/Safety
2010-10-27T23:22:41Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
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display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- Background Contents End ---><br />
<!--- Main Contents Start ---><br />
<html><br />
<body><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Safety issue</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<strong><font size="2">Would any of your project ideas raise safety issues in terms of: </strong></font><br><br />
- Our theme is double click system in bacteria and we expect that it will be used for eliminating false input.<br> <br />
It can raise accuracy of work to the target and reduce side effect by responding double click.<br><br />
We hope this system to be used for safety device on labs.<br><br><br />
<strong><font size="2"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
</strong></font><br><br />
- We've desinged pulse generator and some hybrid promoters. We've also registed the parts.<br><br />
We think construction of these parts can be used as safety device.<br><br />
For example, hybrid promoter is low unregulated and repression for the promoter is stronger than activation for.<br><br />
So, expression of protein occurs in specific environment which can mean safety device.<br><br />
If reaction needs the protein expressed by pulse, the pulse also can be safety device.<br><br />
Because, reaction must be done in fixed time whose the protein exists in. <br><br><br />
</td><br />
</tr><br />
</table><br />
</body><br />
</html><br />
<br />
<br />
[[Safety |Go to safety page]].</div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T23:08:12Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
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<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
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<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="3" face=verdana><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br>Kaoru Yamamoto<br />
<br>Jyun Kajiwara<br />
<br><br />
<br><br />
<br></font></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem
Team:Chiba/System 2/Evaluation subsystem
2010-10-27T23:02:47Z
<p>Hotpotato: </p>
<hr />
<div><!-- ---------------------------------------------------------<br />
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</head><br />
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<body><br />
<div id="tabs10"><br />
<ul><br />
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
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<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
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<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Testing_individual_parts"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Remarks"><span>Remarks</span></a></li><br />
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<font size=6>System 2 :</font></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2/Construction_Process
Team:Chiba/System 2/Construction Process
2010-10-27T23:02:15Z
<p>Hotpotato: </p>
<hr />
<div><!-- ---------------------------------------------------------<br />
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<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
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background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
margin-top:0px;<br />
}<br />
#tabs9 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs9 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs9 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
#tabs8 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#007500;<br />
margin-top:0px;<br />
}<br />
#tabs8 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs8 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs8 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs8 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Testing_individual_parts"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
<!--- 3rd tab End ---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<br><br><br><br><br><br><br />
<font size=6>System 2 :</font></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2/Construction_Process
Team:Chiba/System 2/Construction Process
2010-10-27T23:01:19Z
<p>Hotpotato: </p>
<hr />
<div><!-- ---------------------------------------------------------<br />
--------------------------------------------------------------<br />
下の方に「ここから下を編集」というところがあります<br />
そっちを編集してください<br />
--------------------------------------------------------------<br />
------------------------------------------------------------ --><br />
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
<html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
margin-top:0px;<br />
}<br />
#tabs9 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs9 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs9 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
#tabs8 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#007500;<br />
margin-top:0px;<br />
}<br />
#tabs8 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs8 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs8 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs8 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Testing_individual_parts"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
<!--- 3rd tab End ---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<br><br><br><br><br><br><br />
<font size=6>System 1 :</font></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2/Testing_components
Team:Chiba/System 2/Testing components
2010-10-27T23:00:49Z
<p>Hotpotato: </p>
<hr />
<div><html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
<html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
margin-top:0px;<br />
}<br />
#tabs9 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs9 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs9 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
#tabs8 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#007500;<br />
margin-top:0px;<br />
}<br />
#tabs8 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs8 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs8 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs8 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Testing_components"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
<!--- 3rd tab End ---><br />
<br><br><br><br><br><br><br />
<font size=6>System 2 :</font></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_2
Team:Chiba/System 2
2010-10-27T22:59:17Z
<p>Hotpotato: </p>
<hr />
<div><!-- ---------------------------------------------------------<br />
--------------------------------------------------------------<br />
下の方に「ここから下を編集」というところがあります<br />
そっちを編集してください<br />
--------------------------------------------------------------<br />
------------------------------------------------------------ --><br />
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
<html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
margin-top:0px;<br />
}<br />
#tabs9 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs9 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs9 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
#tabs8 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#007500;<br />
margin-top:0px;<br />
}<br />
#tabs8 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs8 li {<br />
float:left;<br />
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<font size=6>System 2 :</font><br />
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<td width="965px"><font size="4" face="verdana">Overall Circuit</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/1/1a/Chiba_Sys2.jpg"><br><br />
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Our second circiut of double click system is called <b>Recognize circuit</b>.<br><br />
It consists of <b>recognizing input</b> system and <b>memorizing input</b> system.<br />
</td><br />
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<td width="965px"><font size="4" face="verdana">Control of circuit</font><br><br />
<hr width="500" size="1" align="left"><br><br />
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<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/9/99/Chiba_Sys2_Input.jpg"><br><br />
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<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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We use AHL for input and regard injection of AHL as clicking. LuxR is generated constitutive. Injecting AHL to Double Click Bacteria brings dimer of AHL and luxR. We take lux promoter repressed by the dimer. As the dimer binds Plux, transcription of proteins under the Plux is repressed.<br><br />
So in our project, <br><br />
<b><font color="red">CLICK controls transcription of overall DNA sequence.</font></b><br />
</td><br />
<td width="400px"><center><br />
<img src="https://static.igem.org/mediawiki/2010/e/e9/Chiba_LuxR.jpg"></center><br />
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<font size="4" face="verdana">Recognizing Input System</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<img src="<br />
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In recognizing input system, there is a cI operator above gfp DNA sequence. If cI binds cI operator, transcription of gfp is stopped. You can see DNA sequence of cI, which a kind of repressor, is under the Plux. On initial state(there isn't any click), cI is generated normally. But AHL come into Double Click Bacteria, generation of cI is stopped. Since repression of Plux starts working by the dimer which is a combination of AHL and LuxR. And then, cI is going to disappear, because all kinds of proteins are bound to be decomposed. If there isn't cI anymore, bacteria will have chance to shine.<br><br />
We can say<br><br />
<b><font color="red">cI recognizes the existence of input</font></b> in this DNA sequence.</td><br />
<td width="400px"><center><br />
<img src="https://static.igem.org/mediawiki/2010/9/90/Chiba_cI.jpg"></center><br />
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<table border="0" cellpadding="30" cellspacing="0"> <br />
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<td width="965px"><br />
<font size="4" face="verdana">Memorizing Input System</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/2/26/Chiba_Sys2_AND.jpg"><br><br />
</center><br />
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<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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In memorizing system, there is an AND Gate with T7ptag and supD. As you know, T7ptag becomes T7 polymerase in case of existing supD. T7 polymerase is translated to T7 protein which works as activator of T7 promoter. There is a T7 promoter above gfp DNA sequence. If T7 have been generated once, the T7 promoter starts translating. And then we can see bacteria begin to shine.<br><br><br />
<br />
<br />
<br />
On initial state, bacteria don't have supD. Because, lacI represses the lac promoter above supD DNA sequence.So there isn't any T7 polymerase and gfp cannot be genernated, in spite of being T7ptag in bacteria.<br><br><br />
<br />
<br />
<br />
As written above, when AHL is entered into bacteria, transcription of proteins under the Plux is repressed. If there is click, transcription of T7ptag and lacI are stopped simultaneously. But T7ptag are decomposed earlier than lacI because of difference in velocity of decomposition. T7ptag which is a kind of mRNA is decomposed so fast and lacI which is just a kind of protein is decomposed late. SupD under the Plac is generated after most of lacI are decomposed. So, during the clicking, there isn't any T7ptag as far as there is supD. <br><br><br />
<br />
<br />
<br />
After washing AHL(means after 1st click), supD exist. SupD is a kind of tRNA and stable which means having long half-life. If there isn't input anymore, Plux starts activating and T7ptag is generated. As result of reacting T7ptag and supD, the AND Gate is going to be available. In this case, <b><font color="red">the AND Gate remembers that there was a click.</font></b><br><br><br />
<br />
<br />
Moreover, if there is 2nd click, T7ptag is going to be decomposed again. And then, the reaction of And Gate is stopped, because there isn't T7ptag anymore. So, T7 which is activator of T7 promoter is also going to be decomposed. It is time-limit of Double-Click and explain it in next paragraph. </td><br />
<td width="400px"><center><br />
<img src="https://static.igem.org/mediawiki/2010/6/6e/Chiba_third.jpg"></center><br />
</td><br />
</tr><br />
</table><br />
<br />
<br />
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<table border="0" cellpadding="30" cellspacing="0"> <br />
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<td width="965px"><br />
<font size="4" face="verdana">Co-working of two systems </font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<img src="<br />
https://static.igem.org/mediawiki/2010/b/b3/Chiba_Sys2_Whole.jpg"><br><br />
</center><br />
</td><br />
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</table><br />
<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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<td width="900px"><font size=2 face=verdana><br />
Actually, there is a hybrid promoter(cI/T7) above gfp DNA sequence. It is regulated by T7 and cI which work as activator and repressor, respectively.<br />
On this promoter, repression is stronger than activation. And also, it is low unregulated activation. <font color="red"><b>When 1st input</b></font> is come in, cI recognizes the input and going to be decomposed.<br />
But there isn't T7 yet, <font color="red"><b>gfp cannot be generated</b></font>, despite there isn't repressor. <font color="red"><b>After 1st input,</b></font> AND gate remembers there was a click, and generation of T7 is stared. But, <font color="red"><b>gfp cannot be generated</b></font>, because there is cI already. <font color="red"><b>When 2nd input</b></font> is come, cI also recognizes the input and going to be decomposed. If the 2nd input comes within time that the AND is working, T7 exists in bacteria and decomposition of cI is finished, <font color="red"><b>gfp is going to be generated.</b></font>As a result, bacteria shines with gfp and Double Click is completed.</td><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:56:34Z
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<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
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<font size="3" face=verdana><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
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Hotpotato
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Team:Chiba/Support
2010-10-27T22:55:35Z
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<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px">===<font size="5" face="verdana">Our supporters</font>===<br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px">===<font size="5" face="verdana">Thanks to</font>===<br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="3" face=verdana><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br></font></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:54:49Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="3" face=verdana><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br></font></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:54:20Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
===<td width="800px"><font size="5" face="verdana">Our supporters</font>===<br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
===<td width="800px"><font size="5" face="verdana">Thanks to</font>===<br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="3" face=verdana><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br></font></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:53:31Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="3" face=verdana><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br></font></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:44:52Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:43:48Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</tr><br />
<tr><td><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br></td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:42:56Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</tr><br />
<tr><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:34:53Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><<br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<td><br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br><br />
</td><br />
</tr><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:33:15Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
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<br />
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#tabs10 #current a span {<br />
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--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><<br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<br>Robert Sidney Cox III<br />
<br>Kan Tanaka<br />
<br>Kyoichi Saito<br />
<br><br />
<br>Yohei Tashiro<br />
<br>Ryo Ishihara<br />
<br>Akinori Katabami<br />
<br>Mayu Ikezumi<br />
<br>Akira Tanaka<br />
<br>Masahiro Tominaga<br />
<br>Hiroki Hukutomi<br />
<br><br />
<br><br />
<br><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/Support
Team:Chiba/Support
2010-10-27T22:25:30Z
<p>Hotpotato: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
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float:left;<br />
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display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- Background Contents End ---><br />
<br />
<!--- Main Contents Start ---><br />
<html><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Our supporters</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="200px"><center><br />
<a href="http://mrgene.com/"><img src="https://static.igem.org/mediawiki/2010/4/4c/Mrgene.gif" width="80px"></a><br />
</td><br />
<td width="200px"><br />
<a href="http://www.geneart.com/"><img src="https://static.igem.org/mediawiki/2010/6/68/Geneart~chiba.gif" ALT = "" width="180px"></A><<br />
</td><br />
<td width="200px"><br />
<a href="http://kserv.jp/"><img src="https://static.igem.org/mediawiki/2010/3/37/Knowledge.jpg" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.neo-morgan.com/"><img src = "https://static.igem.org/mediawiki/2010/a/a7/Neomorgan.png" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.greiner-bio-one.co.jp/top.html"><img src="https://static.igem.org/mediawiki/2010/4/47/Greiner.gif" ALT = ""></A><br />
</td><br />
<td><br />
<a href="http://www.cosmobio.co.jp/"><img src="https://static.igem.org/mediawiki/2010/b/b8/Cosmo.jpg"></a><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://www.yakukensha.co.jp/"><img src = "https://static.igem.org/mediawiki/2010/0/01/Yakukensha.gif" ALT = "" width="180px"></A> <br />
</td><br />
<td><br />
<a href="<br />
http://www.home.agilent.com/"><img src = "https://static.igem.org/mediawiki/2010/5/53/Chiba_agilent.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="http://www.eppendorf.com/"><img src = "https://static.igem.org/mediawiki/2010/e/e9/Chiba_eppendorf.gif<br />
" ALT = "" width="180px"></A><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<a href="http://chiba-kougaku-dosokai.jp/"><img src="https://static.igem.org/mediawiki/2010/5/5b/Kougaku.gif" width="180px"></a><br />
</td><br />
<td><br />
<a href="http://www.chiba-u.ac.jp/"><IMG SRC = "https://static.igem.org/mediawiki/2010/a/a0/Chiba-u.gif" ALT = "" width="180px"></A><br />
</td><br />
<td><br />
<a href="<br />
http://www.vbl.chiba-u.jp/index_jp.htm"><img src="https://static.igem.org/mediawiki/2010/9/9d/Chiba_VBL.jpg" width="180px"></a> <br />
<br />
</td><br />
</tr><br />
</center><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0"> <br />
<tr><br />
<td width="800px"><font size="5" face="verdana">Thanks to</font><br><br />
<hr width="500" size="1" align="left"><br><br />
</table><br />
</html><br />
<!--- Main Contents End ---></div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-27T22:08:12Z
<p>Hotpotato: </p>
<hr />
<div><!-- ---------------------------------------------------------<br />
--------------------------------------------------------------<br />
下の方に「ここから下を編集」というところがあります<br />
そっちを編集してください<br />
--------------------------------------------------------------<br />
------------------------------------------------------------ --><br />
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
<html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
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text-decoration:none;<br />
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#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
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<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
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#tabs9 ul {<br />
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padding:2px 10px 0 105px;<br />
list-style:none;<br />
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float:left;<br />
display:inline;<br />
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padding:0;<br />
margin-left:40px;<br />
}<br />
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float:left;<br />
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padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
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===<font size="5">Testing individual parts</font>===<br />
-----<br />
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input.<br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
For GFP generator, the parts shown below were checked.<br />
<br />
1. Constitutive promoter<br><br />
2. LuxR<br> <br />
3. T7/CI-OR1 hybrid promoter<br />
<br />
<br />
For Pulse generator, the parts shown below were checked.<br />
<br />
1. Lux/CI434 hybrid promoter<br> <br />
2. Pulse generator<br />
<br />
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
<br><br><br><br />
<br />
===<font size="5">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
About the design<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
===<font size="5">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Testing_components
Team:Chiba/System 1/Testing components
2010-10-27T22:07:45Z
<p>Hotpotato: </p>
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<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
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===<font size="5">Testing individual parts</font>===<br />
-----<br />
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input.<br />
The subparts of those plasmids are shown below.<br><br />
<center><br />
[[Image:Chiba_icon_1.jpg]]<br><br />
[[Image:Chiba_icon_2.jpg]]<br><br />
</center><br />
Regarding with those two plasmids, we tested the function of each parts in the plasmids.<br><br />
<br />
For GFP generator, the parts shown below were checked.<br />
<br />
1. Constitutive promoter<br><br />
2. LuxR<br> <br />
3. T7/CI-OR1 hybrid promoter<br />
<br />
<br />
For Pulse generator, the parts shown below were checked.<br />
<br />
1. Lux/CI434 hybrid promoter<br> <br />
2. Pulse generator<br />
<br />
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.<br />
<br />
<br><br><br><br />
<br />
===<font size="5">In detail</font>===<br />
-----<br />
Hybrid promoter Design <br />
<br />
We got the idea that cI and cI434 repressor don’t crosstalk[1], so we construct Pt7/cI and pLux/cI434 hybrid promoter.<br />
<br />
About the design<br />
--------<br />
1. pLux/cI434 hybrid promoter <br />
<br />
Rationale <br />
This hybrid promoter is designed to activated by LuxR when it is bound to AHL and repressed by the phage 434 cI repressor. The operator site sequence come from the biobrick part (BBa_R0052) and we set OR2(ttacaatgtatcttg) between -35 and -10, OR1(ttacaaactttcttg) downstream of -10. There are only 16 bp between the -10 and -35 boxes we have confirmed that lux promoter works naturally while repression is under observation. <br />
<br />
<br><br><br><br />
===<font size="5">References and links</font>===<br />
-----<br />
[1] http://web.mit.edu/synbio/x/htdocs/BB_Parts/BBa_R0050/Part.htm<br />
[2] http://partsregistry.org/Part:BBa_I12040<br />
[3] Basu S, Mehreja R, Thiberge S, Chen MT and Weiss R (2004). Spatiotemporal <br><br />
control of gene expression with pulse-generating networks. Proc Natl Acad Sci USA 101:<br><br />
6355-6360.</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Construction_Process
Team:Chiba/System 1/Construction Process
2010-10-27T22:06:45Z
<p>Hotpotato: /* Construction process of GFP generator plasmid */</p>
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<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
margin-top:0px;<br />
}<br />
#tabs9 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs9 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs9 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
#tabs8 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#007500;<br />
margin-top:0px;<br />
}<br />
#tabs8 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs8 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs8 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs8 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
</html><br />
<!--- 3rd tab End ---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
-------------------------------------------------------------------------------<br />
ここから下を編集<br />
-------------------------------------------------------------------------------<br />
----------------------------------------------------------------------------------- --><br />
<br><br><br><br />
<br><br><br><br />
===<font size="5">Construction process of GFP generator plasmid</font>===<br />
-----<br />
<br><br><br><center><br />
[[Image:Chiba_gfpgen.jpg]]</center><br />
<br><br><br><br />
<br />
===<font size="5">Construction process of Pulse generator plasmid</font>===<br />
-----</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Construction_Process
Team:Chiba/System 1/Construction Process
2010-10-27T22:06:28Z
<p>Hotpotato: /* Construction process of GFP generator plasmid */</p>
<hr />
<div><!-- ---------------------------------------------------------<br />
--------------------------------------------------------------<br />
下の方に「ここから下を編集」というところがあります<br />
そっちを編集してください<br />
--------------------------------------------------------------<br />
------------------------------------------------------------ --><br />
<html><link rel="stylesheet" href="https://2010.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html><br />
<html><br />
<style type="text/css"><br />
#top-section {<br />
height: 0px;<br />
margin-top: -80px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
margin-bottom: 0 !important;<br />
margin-bottom: 0px;<br />
padding:0;<br />
border: 0;<br />
}<br />
</style><br />
</html><br />
<!--- Background Contents ---><br />
<!--- 1st tab ---><br />
<html><br><br><br><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<br />
<br />
<body bgcolor="blue" text="black" link="yellow" vlink="purple" alink="red" background="https://static.igem.org/mediawiki/2010/a/a1/Chiba_ground_2.jpg"><br />
<img src="https://static.igem.org/mediawiki/2010/f/f9/Chiba_menu_2.jpg"><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 10--------------------------- */<br />
<br />
#tabs10 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#373737;<br />
margin-top:-10px;<br />
}<br />
#tabs10 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs10 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs10 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs10 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs10 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs10 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs10 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs10 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs10"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li id="current"><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
<br />
</ul><br />
</div><br />
<!--- 1st tab End ---><br />
<!--- 2nd tab ---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 9--------------------------- */<br />
<br />
#tabs9 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#125FDB;<br />
margin-top:0px;<br />
}<br />
#tabs9 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs9 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs9 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs9 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs9 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs9 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs9 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs9 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs9 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs9"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
</div><br />
</body><br />
<!--- 2nd tab End---><br />
<!--- 3rd tab---><br />
<div id="search-controls"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
<p><br />
<style type="text/css"> <br />
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"><br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin:0;<br />
padding:0;<br />
font: bold 11px/1.5em Verdana;<br />
}<br />
<br />
h2 {<br />
font: bold 14px Verdana, Arial, Helvetica, sans-serif;<br />
color: #000;<br />
margin: 0px;<br />
padding: 0px 0px 0px 15px;<br />
}<br />
<br />
img {<br />
border: none;<br />
}<br />
<br />
/*- Menu Tabs 8--------------------------- */<br />
<br />
#tabs8 {<br />
float:left;<br />
width:100%;<br />
font-size:100%;<br />
line-height:normal;<br />
background:#007500;<br />
margin-top:0px;<br />
}<br />
#tabs8 ul {<br />
margin:0;<br />
padding:2px 10px 0 105px;<br />
list-style:none;<br />
}<br />
#tabs8 li {<br />
float:left;<br />
display:inline;<br />
margin:0;<br />
padding:0;<br />
margin-left:40px;<br />
}<br />
#tabs8 a {<br />
float:left;<br />
background:url("tableft10.gif") no-repeat left top;<br />
margin:0;<br />
padding:0 0 0 2px;<br />
text-decoration:none;<br />
}<br />
#tabs8 a span {<br />
float:left;<br />
display:block;<br />
background:url("tabright10.gif") no-repeat right top;<br />
padding:5px 15px 4px 6px;<br />
color:#FFF;<br />
}<br />
/* Commented Backslash Hack hides rule from IE5-Mac \*/<br />
#tabs8 a span {float:none;}<br />
/* End IE5-Mac hack */<br />
#tabs8 a:hover span {<br />
color:#FFFFFF;<br />
}<br />
#tabs8 a:hover {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 a:hover span {<br />
background-position:100% -42px;<br />
}<br />
<br />
#tabs8 #current a {<br />
background-position:0% -42px;<br />
}<br />
#tabs8 #current a span {<br />
background-position:100% -42px;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
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<br><br><br><br />
<br><br><br><br />
==<font size="5">Construction process of GFP generator plasmid</font>==<br />
-----<br />
<br><br><br><center><br />
[[Image:Chiba_gfpgen.jpg]]</center><br />
<br><br><br><br />
<br />
===<font size="5">Construction process of Pulse generator plasmid</font>===<br />
-----</div>
Hotpotato
http://2010.igem.org/Team:Chiba/System_1/Construction_Process
Team:Chiba/System 1/Construction Process
2010-10-27T22:05:48Z
<p>Hotpotato: /* Construction process of GFP generator plasmid */</p>
<hr />
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<div id="tabs8"><br />
<ul><br />
<!-- CSS Tabs --><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li><br />
</ul><br />
</div><br />
</body><br />
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<!--- 3rd tab End ---><br />
<!--- Background Contents End ---><br />
<!-- -------------------------------------------------------------------------------<br />
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<br><br><br><br />
<br><br><br><br />
===<font size="5">Construction process of GFP generator plasmid</font>===<br />
-----<br />
<br><br><br><center><br />
[[Image:Chiba_gfpgen.jpg]]</center><br />
<br><br><br><br />
<br />
===<font size="5">Construction process of Pulse generator plasmid</font>===<br />
-----</div>
Hotpotato
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-27T21:58:31Z
<p>Hotpotato: </p>
<hr />
<div><!--- Background Contents ---><br />
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a><br />
<ul style="z-index:1"> <br><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a><br />
<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Project">Overall Project</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_1">Circuit 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">Circuit 2</a></li><br />
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<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a><br />
<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid1">Plasmid 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid2">Plasmid 2</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/lux_promoter">lux Promoter</a></li><br><br />
</ul><br />
</li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a><br />
<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook">Notebook 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook_2">Notebook 2</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook_3">Notebook 3</a></li><br><br />
</ul><br />
</li><br />
<br />
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li><br />
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<a href="http://chem.tf.chiba-u.jp/igem/"><img src="https://static.igem.org/mediawiki/2010/d/d7/Chiba_japanpage.jpg" width="100"></a><br />
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<td width="620px"><font size="5" face=verdana>PROJECT DESCPIRTION</font><br><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="2" face=verdana>We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font></td> <br />
<td width="300px"><center><br><br><br />
<img src="https://static.igem.org/mediawiki/2010/f/ff/Chiba_ani_1.gif"></center></td><br />
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</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
<tr><br />
<td width="900px"><center><br />
<p align="center"><br />
<a href="https://2010.igem.org/Team:Chiba/Support"><img src="https://static.igem.org/mediawiki/2010/2/20/ForTOP.png" width="850px"><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/test
Team:Chiba/test
2010-10-27T21:57:57Z
<p>Hotpotato: </p>
<hr />
<div><!--- Background Contents ---><br />
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a><br />
<ul style="z-index:1"> <br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Team">Team</a></li> <li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Member">Member</a></li><br />
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">Circuit 2</a></li><br />
</ul><br />
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<ul style="z-index:1"><br><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid1">Plasmid 1</a></li><br />
<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid2">Plasmid 2</a></li><br />
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<td width="620px"><font size="5" face=verdana>PROJECT DESCPIRTION</font><br><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size="2" face=verdana>We daily double-click the icons to open the files or to exert the program: this is clearly distinguished from the single click, which is often for selecting or highlighting the program. This year, iGEM CHIBA is constructing genetic double-click system whose output is released only when the input (inducing agent) is given twice within a limited time. To discriminate double-click from two separated single-clicks, the 1st input is to be memorized temporarily. If the 2nd input is added before the memory gets lost, output will be produced. If the 2nd input is not added within the given time, the system will be reset to the original state. This mechanism could work as a sort of safety device; by requiring the 2nd ‘confirmation’ input, one can drastically reduce, or even eliminate, the frequency of false-inputs. This system could be useful in operating the potent or potentially-hazardous biochemical processes.<br />
</font></td> <br />
<td width="300px"><center><br><br><br />
<img src="https://static.igem.org/mediawiki/2010/f/ff/Chiba_ani_1.gif"></center></td><br />
</tr><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" align="center"> <br />
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<p align="center"><br />
<a href="https://2010.igem.org/Team:Chiba/Support"><img src="https://static.igem.org/mediawiki/2010/2/20/ForTOP.png" width="850px"><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:45:07Z
<p>Hotpotato: </p>
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
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“Genetic double click system” is based on the AND-gate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. In our first design, we decided a pulse-generator to memorize the input temporarily. And, we designed the system which is based on combination of a Fast-pulse and a Slow-pulse in principle as shown in the following figure. <br><br><br />
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This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br><br />
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At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br><br />
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<td width="965px"><font size="4" face="verdana">Slow Pulse</font><br><br />
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Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br><br />
<center><br />
<br><br><img src="https://static.igem.org/mediawiki/2010/9/91/Chiba_planB_4.jpg" width="500px"><br><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:43:32Z
<p>Hotpotato: </p>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li> </ul><br />
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
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“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. In our first design, we decided a pulse-generator to memorize the input temporarily. And, we designed the system which is based on combination of a Fast-pulse and a Slow-pulse in principle as shown in the following figure. <br><br />
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This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
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<td width="965px"><font size="4" face="verdana">Fast Pulse</font><br><br />
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<img src="https://static.igem.org/mediawiki/2010/4/41/Chiba_planB_2.jpg" width="800px"><br><br />
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At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
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<td width="965px"><font size="4" face="verdana">Slow Pulse</font><br><br />
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Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
<center><br />
<br><br><img src="https://static.igem.org/mediawiki/2010/9/91/Chiba_planB_4.jpg" width="500px"><br><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:38:53Z
<p>Hotpotato: </p>
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size=2 face=verdana><br />
“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. <br><br />
<br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/6/60/Chiba_ani3.gif"><br><br />
</center><br />
In our first design, we decided a pulse-generator to memorize the input temporarily.<br />
And, we designed the system which is based on combination of a Fast-pulse and a Slow-pulse in principle as shown in the following figure.<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg"><br><br />
</center><br />
<br><br>Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
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<td width="965px"><font size="4" face="verdana">Fast Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/4/41/Chiba_planB_2.jpg" width="800px"><br><br />
</center><br />
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At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
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<td width="965px"><font size="4" face="verdana">Slow Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/2/2f/Chiba_planB_3.jpg" width="800px"><br><br />
</center><br />
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<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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<td width="800px"><font size=2 face=verdana><br />
Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
<center><br />
<br><br><img src="https://static.igem.org/mediawiki/2010/9/91/Chiba_planB_4.jpg" width="500px"><br><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:33:41Z
<p>Hotpotato: </p>
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<font size=2 face=verdana><br />
“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. <br><br />
<br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/6/60/Chiba_ani3.gif"><br><br />
</center><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg"><br><br />
</center><br />
<br><br>Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
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<td width="965px"><font size="4" face="verdana">Fast Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/4/41/Chiba_planB_2.jpg" width="800px"><br><br />
</center><br />
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<td width="800px"><font size=2 face=verdana><br />
At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
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<td width="965px"><font size="4" face="verdana">Slow Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/2/2f/Chiba_planB_3.jpg" width="800px"><br><br />
</center><br />
</td><br />
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<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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<td width="800px"><font size=2 face=verdana><br />
Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
<center><br />
<br><br><img src="https://static.igem.org/mediawiki/2010/9/91/Chiba_planB_4.jpg" width="500px"><br><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:32:39Z
<p>Hotpotato: </p>
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
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“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. <br><br />
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<br><br>Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
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At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
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Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:31:48Z
<p>Hotpotato: </p>
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
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“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. <br><br />
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Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
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At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
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Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:29:50Z
<p>Hotpotato: </p>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li><br />
<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li><br />
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<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
<hr width="500" size="1" align="left"><br><br />
“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. <br><br />
<br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/6/60/Chiba_ani3.gif"><br><br />
</center><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg"><br><br />
Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
</center><br />
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<td width="965px"><font size="4" face="verdana">Fast Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/4/41/Chiba_planB_2.jpg" width="800px"><br><br />
</center><br />
</td><br />
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</table><br />
<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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<td width="800px"><font size=2 face=verdana><br />
At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
</td><br />
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<br />
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<td width="965px"><font size="4" face="verdana">Slow Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/2/2f/Chiba_planB_3.jpg" width="800px"><br><br />
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</table><br />
<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
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<td width="800px"><font size=2 face=verdana><br />
Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/9/91/Chiba_planB_4.jpg" width="500px"><br><br />
</center><br />
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Hotpotato
http://2010.igem.org/Team:Chiba/System_1
Team:Chiba/System 1
2010-10-27T21:29:02Z
<p>Hotpotato: Undo revision 197252 by Hotpotato (Talk)</p>
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Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
</center><br />
<td width="800px"><font size="4" face="verdana">Overall Circuit</font><br><br />
<hr width="500" size="1" align="left"><br><br />
“Genetic double click system” is based on the ANDgate which is high only when same inputs are given two times in quick succession. <br />
This system requires the operations which never happen naturally to turn the switch. <br />
So, we believe that this is very safety device. <br />
This system requires that a first input is transient in order to allow a second input. <br />
Unlike chemical inputs, light inputs are very suitable for this system because it does not remain after inputting. But, there is not light sensor with good sensitivity. So, we produced artificially transient input by AHL inputting and washing. <br />
Next, this system is required to memorize the transient first input since this system is based on AND gate. Moreover, this system requires time-limit in genetic sequence between the first and the second input like a computer mouse to recognized double-click and two separated single-clicks as different, so, this system is required to memorize the transient first input temporarily. <br><br />
<br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/6/60/Chiba_ani3.gif"><br><br />
</center><br />
<center><br />
<img src="https://static.igem.org/mediawiki/2010/d/db/Chiba_plan2_1.jpg"><br><br />
Our first design of genetic double-click system is based on a combination of a Fast-pulse and a Slow-pulse. This system consists of a pulse-generator and two inverters which can be seemed as a slow pulse.In this system, we also use AHL input and GFP output. This time, we use AHL as an activate signal, so when there is AHL added, Lux promoter will be activated. The transcription factors of output are T7 RNA Polymerase and cI repressor.<br><br />
</center><br />
</td><br />
</tr><br />
</table><br />
<br />
<br />
<table border="0" cellpadding="30" cellspacing="0" align=”center”> <br />
<tr><br />
<td width="965px"><font size="4" face="verdana">Fast Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/4/41/Chiba_planB_2.jpg" width="800px"><br><br />
</center><br />
</td><br />
</tr><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
<tr><br />
<td width="800px"><font size=2 face=verdana><br />
At initial state, LuxR and cI protein are constitutively generated. cI binds to the operator site of PT7/cI. When 1st input is injected, LuxR-AHL dimmer binds to the Lux-box of the lux promoters so that T7 RNA Polymerase, cI434 and tetR protein are generated at the same time. cI434 gradually accumulates, and gradually repress the transcription of T7 RNA Polymerase so that the expression of T7 RNA Polymerase can be shown as a pulse. At the same time, Transcription of cI is stopped by tetR, cI decomposes and the PT7/cI promoter is unbound. This derepression occurs after the pulse of T7 RNAP has passed. In other words, the operator sites of PT7/cI is repressed by cI when there is pulse of T7 RNA Polymerase. So, it cannot transcribe GFP. TetR creates a time delay here from input to derepression. Because of this time delay and one-time pulse, bacteria can never work by one input. <br />
</td><br />
</tr><br />
</table><br />
<br />
<br />
<br />
<table border="0" cellpadding="30" cellspacing="0" align=”center”> <br />
<tr><br />
<td width="965px"><font size="4" face="verdana">Slow Pulse</font><br><br />
<hr width="500" size="1" align="left"><br><br />
<center><br />
<font size=2 face=verdana><br />
<img src="https://static.igem.org/mediawiki/2010/2/2f/Chiba_planB_3.jpg" width="800px"><br><br />
</center><br />
</td><br />
</tr><br />
</table><br />
<table border="0" cellpadding="30" cellspacing="0" margin-top="-50"> <br />
<tr><br />
<td width="800px"><font size=2 face=verdana><br />
Before injecting 2nd input, we must create none-input-environment. So we choose to wash the 1st input. By washing , tetR protein and cI434 protein will degrade. cI434 protein should disappear so that when there is 2nd input, T7 RNA Polymerase will be shown as a pulse which is the same as the 1st time. cI will begin to generate if tetR protein gets lost. We recognize it as time-limit, when there is enough cI generated (this means cI repression is stronger than T7 RNA Polymerase activation), there will be no GFP output. On the contrary, when there is less cI protein or no cI protein at the moment, T7 RNA Polymerase pulse can accumulate GFP output. By the second injected AHL before the inhibition by cI, T7 RNA Polymerase binds to the PT7/cI promoter and transcribes the downstream GFP. <br />
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