User contributions
From 2010.igem.org
(Latest | Earliest) View (newer 500 | older 500) (20 | 50 | 100 | 250 | 500)
- 17:11, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 17:09, 9 September 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 17:04, 9 September 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 17:02, 9 September 2010 (diff | hist) Team:Groningen/Chaplins (top)
- 14:59, 9 September 2010 (diff | hist) N Team:Groningen/Chaplins (New page: '''Expression Chaplins in NZ8900''' -Grow O/N culture in Ty with antibiotics (Km5 and Cm5) -Measure OD600 (with 0.9 ml with 100 μL of O/N culture, number *10=OD) -Inoculate 15ml TY (wi...)
- 14:57, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 14:55, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 14:51, 9 September 2010 (diff | hist) N Procedures for Bacillus subtilis 168 (New page: '''2-step transformation procedure for ''Bacillus subtilis'' 168''' *Cells are grown O/N at 37 °C in supplemented medium contains Spizizen's salts (without antibiotics) *Dilute ce...) (top)
- 14:49, 9 September 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 18:07, 31 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 17:37, 31 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 16:47, 31 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 19:11, 28 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 17:54, 28 August 2010 (diff | hist) N Team:Groningen/2-step Transformation Procedure for Bacillus subtilis 168 (New page: '''2-step transformation procedure for ''Bacillus subtilis'' 168''' *Cells are grown O/N at 37 °C in supplemented medium contains Spizizen's salts (without antibiotics) *Dilute ce...) (top)
- 17:54, 28 August 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 17:53, 28 August 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 23:48, 24 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 23:02, 24 August 2010 (diff | hist) Team:Groningen/Growing Streptomyces
- 22:44, 24 August 2010 (diff | hist) Team:Groningen/CaCl2 Competent Cells (top)
- 22:32, 24 August 2010 (diff | hist) N TY medium (New page: '''Ingredients''' -10 g Bacto-tryptone -5 g yeast extract -10 g NaCl '''Protocol''' -Mix dry ingredients and add distilled water up to 1 Liet -Pour into 2 L flask (or greater) -Auto...) (top)
- 22:30, 24 August 2010 (diff | hist) N Starvation medium (New page: Starvation medium: 50 ml 1X Spizizen's salts+ 0.5% glucose (better not to add antibiotics)) (top)
- 22:28, 24 August 2010 (diff | hist) N Supplemented medium (New page: *50 ml 1X diluted '''Spizizen's salts''' *0.5 ml(0.02%) '''casamino acids''' (2%) *0.5 ml(14 µg/ml) '''tryptophane''' (100X) *1.25 ml (0.5%)'''glucose''' (20%) *50 µl (22 µg/ml) '''FeN...) (top)
- 20:12, 24 August 2010 (diff | hist) N Spizizen's salts (New page: Spizizen's 5X diluted salts solution contains of (per liter): *K2HPO4, 14.8 g/l (85 mM) *KH2PO4, 5.4 g/l (40 mM) *(NH4)2SO4, ...) (top)
- 20:03, 24 August 2010 (diff | hist) Team:Groningen/Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
- 19:46, 24 August 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 19:44, 24 August 2010 (diff | hist) Team:Groningen/Protocols
- 13:58, 24 August 2010 (diff | hist) Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
- 13:48, 24 August 2010 (diff | hist) Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
- 13:26, 24 August 2010 (diff | hist) N Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer (New page: '''Wear gloves at all times!''' Requirements: *30% acrylamide(Biorad) '''NEUROTOXIN!''' *4X separation gel buffer: 1.5M Tris.HCl, 0.4% SDS pH6.8 *4X stacking gel buffer: 0.5M Tris.HCl, 1...)
- 13:22, 24 August 2010 (diff | hist) Team:Groningen/Protocols
- 11:33, 24 August 2010 (diff | hist) Growing Streptomyces (top)
- 11:33, 24 August 2010 (diff | hist) N Growing Streptomyces (New page: -Make plates with a thick layer of medium, 100 ml medium per big Petri dish.Medium of Streptomyces is mannitol soya flour medium (MS or SFM) 300 ml per 1L bottle to avoid boiling over in a...)
- 11:31, 24 August 2010 (diff | hist) Team:Groningen/Protocols (Replacing page with 'CaCl2 Competent Cells Growing Streptomyces')
- 11:28, 24 August 2010 (diff | hist) N Growing streptomyces (New page: -make plates with a thick layer of medium, 100 ml medium per big Petri dish Medium of Streptomyces is mannitol soya flour medium (MS or SFM) 300 ml per 1L bottle to avoid boiling over in ...) (top)
- 11:23, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 11:20, 24 August 2010 (diff | hist) N SOC medium (New page: -Follow directions to make 1 liter of TY media -After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution) (top)
- 11:19, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 10:45, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 10:35, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 10:28, 24 August 2010 (diff | hist) N CaCl2 Competent Cells (New page: Transformation protocol Melt agar in autoclave or microwave. Cool down to 60 oC. One jar ( 100mL ) is enough for 7 plates. -Add 10 μl of ligation product to 100 μl of cells ( don't...)
- 10:14, 24 August 2010 (diff | hist) N LB( TY) medium (New page: '''Ingredients''' -10 g Bacto-tryptone -5 g yeast extract -10 g NaCl '''Protocol''' -Mix dry ingredients and add distilled water up to 1 Liet -Pour into 2 L flask (or greater) -Auto...) (top)
- 09:53, 24 August 2010 (diff | hist) Team:Groningen/Protocols
- 09:51, 24 August 2010 (diff | hist) N Team:Groningen/Protocols (New page: CaCl2 Competent Cells '''Day 1:''' -Inoculate 5 ml LB( TY) medium with ''E.coli'' from glycerol stock -Grow overnight at 37 oC '''Day 2:''' -Inoculate 20 ml LB medium with 200 ...)
- 00:06, 17 August 2010 (diff | hist) N File:This is me.jpg (top)
- 23:59, 30 March 2010 (diff | hist) N Users/Ezgi (New page: ==H.Ezgi KARAKAS== this is me:) Hi! I'm Ezgi. I'm a third year molecular biology and genetics student in METU,Turkey and an exchange student in Rijsuniversitei...) (top)
- 17:15, 29 March 2010 (diff | hist) N File:CIMG0399.JPG (top)
- 16:55, 29 March 2010 (diff | hist) 2010.igem.org:Users (→H. Ezgi Karakas) (top)
- 16:53, 29 March 2010 (diff | hist) N 2010.igem.org:Users (New page: ==''H. Ezgi Karakas''== Image:CIMG0399.jpeg Hello, I'm Ezgi. I'm a third year, molecular biology and genetics student in Middle East Technical University (METU) in Turkey and an exch...)
- 15:45, 23 March 2010 (diff | hist) Talk:Team:Groningen
- 18:07, 15 March 2010 (diff | hist) Talk:Team:Groningen
(Latest | Earliest) View (newer 500 | older 500) (20 | 50 | 100 | 250 | 500)