User contributions
From 2010.igem.org
(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)
- 22:30, 24 August 2010 (diff | hist) N Starvation medium (New page: Starvation medium: 50 ml 1X Spizizen's salts+ 0.5% glucose (better not to add antibiotics)) (top)
- 22:28, 24 August 2010 (diff | hist) N Supplemented medium (New page: *50 ml 1X diluted '''Spizizen's salts''' *0.5 ml(0.02%) '''casamino acids''' (2%) *0.5 ml(14 µg/ml) '''tryptophane''' (100X) *1.25 ml (0.5%)'''glucose''' (20%) *50 µl (22 µg/ml) '''FeN...) (top)
- 20:12, 24 August 2010 (diff | hist) N Spizizen's salts (New page: Spizizen's 5X diluted salts solution contains of (per liter): *K2HPO4, 14.8 g/l (85 mM) *KH2PO4, 5.4 g/l (40 mM) *(NH4)2SO4, ...) (top)
- 20:03, 24 August 2010 (diff | hist) Team:Groningen/Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
- 19:46, 24 August 2010 (diff | hist) Team:Groningen/Protocols (→Protocols)
- 19:44, 24 August 2010 (diff | hist) Team:Groningen/Protocols
- 13:58, 24 August 2010 (diff | hist) Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
- 13:48, 24 August 2010 (diff | hist) Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer
- 13:26, 24 August 2010 (diff | hist) N Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer (New page: '''Wear gloves at all times!''' Requirements: *30% acrylamide(Biorad) '''NEUROTOXIN!''' *4X separation gel buffer: 1.5M Tris.HCl, 0.4% SDS pH6.8 *4X stacking gel buffer: 0.5M Tris.HCl, 1...)
- 13:22, 24 August 2010 (diff | hist) Team:Groningen/Protocols
- 11:33, 24 August 2010 (diff | hist) Growing Streptomyces (top)
- 11:33, 24 August 2010 (diff | hist) N Growing Streptomyces (New page: -Make plates with a thick layer of medium, 100 ml medium per big Petri dish.Medium of Streptomyces is mannitol soya flour medium (MS or SFM) 300 ml per 1L bottle to avoid boiling over in a...)
- 11:31, 24 August 2010 (diff | hist) Team:Groningen/Protocols (Replacing page with 'CaCl2 Competent Cells Growing Streptomyces')
- 11:28, 24 August 2010 (diff | hist) N Growing streptomyces (New page: -make plates with a thick layer of medium, 100 ml medium per big Petri dish Medium of Streptomyces is mannitol soya flour medium (MS or SFM) 300 ml per 1L bottle to avoid boiling over in ...) (top)
- 11:23, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 11:20, 24 August 2010 (diff | hist) N SOC medium (New page: -Follow directions to make 1 liter of TY media -After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution) (top)
- 11:19, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 10:45, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 10:35, 24 August 2010 (diff | hist) CaCl2 Competent Cells
- 10:28, 24 August 2010 (diff | hist) N CaCl2 Competent Cells (New page: Transformation protocol Melt agar in autoclave or microwave. Cool down to 60 oC. One jar ( 100mL ) is enough for 7 plates. -Add 10 μl of ligation product to 100 μl of cells ( don't...)
(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)