http://2010.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=50&target=Mlower2010.igem.org - User contributions [en]2024-03-28T13:49:48ZFrom 2010.igem.orgMediaWiki 1.16.5http://2010.igem.org/Template:TemplatebottomTemplate:Templatebottom2010-10-27T23:53:44Z<p>Mlower: </p>
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</html></div>Mlowerhttp://2010.igem.org/Template:TemplatebottomTemplate:Templatebottom2010-10-27T23:50:43Z<p>Mlower: </p>
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</html></div>Mlowerhttp://2010.igem.org/Team:Warsaw/AttributionTeam:Warsaw/Attribution2010-10-27T23:47:50Z<p>Mlower: </p>
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<h2>Author Attribution</h2><br />
<br />
<br />
<p>Because we had ambitious project and very little time we adopted a<br />
shift based work system in the wet lab. Some team members used to come<br />
early in the morning and after dinner their experiments were passed to<br />
others, who like working late. Our dry lab worked whenever they could.</p><br />
<br />
<h3>Attributions by project:</h3><br />
<br />
<div class="note">1.RBS Measurement</div><br />
<br />
<p>20 constructs needed for this experiment were cloned by: Kasia Grześ,<br />
Ania Puławska, Ania Safray, Cherry Moreno, Milena Bażlekowa, Anna Olchowik.<br />
The lab work was supervised by Jarek Pankowski and Kasia Grześ.<br />
Kasia's skills and patience were crucial for finishing this stage of<br />
the project.</p><br />
<br />
<p>Parts' characterization using FACS measurements were conducted by<br />
Cherry Moreno under the supervision of Michał Lower with some help<br />
from other team members.</p><br />
<p>Parts' characterization using qPCR was performed by Anna Olchowik And<br />
Michał Lower. We'd like also thank Piotr Przanowski from Necki<br />
Institute, who allowed us access qPCR machine in his lab and advised<br />
us about experimental setup.</p><br />
<br />
<div class="note">2.Kill Switch(MinC)</div><br />
<p>This was Jarek Pankowski's idea.</p> <p>Cloned and tested mainly by Kuba<br />
Piątkowski and Jarek Pankowski with a some help from other team<br />
members.</p><br />
<br />
<div class="note">3.Bacto DHL</div><br />
<br />
<p>Constructs containing invasin and listeriolysin were cloned by Marta<br />
Błaszkiewicz and Joanna Leszczyńska under Michał Lower supervision. </p><br />
<p>The<br />
invasion experiments followed by flow cytometry measurements were<br />
performed by Kamil Koper, Anna Olchowik, Michał Lower and Cherry<br />
Moreno. </p><br />
<br />
<p>Microscopic observations were mainly conducted by Dominik<br />
Cysewski and Michał Lower.</p><br />
<p>Dominik's camera is also an important team member, without her we<br />
wouldn't have any pictures.</p><br />
<p>RFC that would help other teams measure cell invasion in the future<br />
was prepared by Anna Olchowik and Michał Lower.</p><br />
<br />
<div class="note">4.Human Practices</div><br />
<p>Ania Puławska, Cherry Moreno, Dominik Cysewski, Joanna Leszcyńska,<br />
Marta Błaszkiewicz, and Milena Bażlekowa prepared the presentation,<br />
went to local high schools and gave lectures about synthetic biology and<br />
iGEM competition. Moreover they prepared a questionnaire for students.</p><br />
<br />
<p>The whole team did brainstorming about<br />
Biobrick ownership. Michał came up with the idea of licensing the<br />
Biobricks like computer programs using creative commons license. Later<br />
with help of Ania Olchowik it was shaped to form of RFC document.</p><br />
<div id="humana"></div><br />
<br />
<div class="note">5. Software</div> <p>Was designed and implemented by Albert Bogdanowicz and<br />
Marta Błaszkiewicz under Michał's Lower supervision.</p><br />
<br />
<div class="note">6. Wiki</div> <p>Team's webpage draft has been coded by Anna Olchowik and<br />
Michał Lower, the final webpage has been recoded by a specialist in the<br />
field - Marta Błaszkiewicz.</p> <p>It wouldn't look nice without Ania<br />
Puławska hand-made pictures. All members of the team contributed to our<br />
wiki.</p><br />
<br />
<div class="note">7. Fundrising</div> <p>Michał Lower, Dominik Cysewski and Anna Olchowik<br />
organized funding.</p><br />
<p> Dominik's efforts need additional acknowledgment<br />
because during the summer he acted as the team's manager and<br />
fundriser. Thanks to him our team have obtained patronage of Polish<br />
Minister of Science.</p><br />
<br />
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</html> <br />
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{{Templatebottom}}</div>Mlowerhttp://2010.igem.org/Template:TemplatebottomTemplate:Templatebottom2010-10-27T23:47:29Z<p>Mlower: New page: <html> </div> </div> <script type="text/javascript"> var data=new Date(); var prog=new Date(2010,9,28,5,59); if (data.getTime()>prog.getTime()) if (document.getElementById("humana")) docum...</p>
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We'd like also to thank Justyna Lesiak who didn't work with us during the summer but in the end posted results of iGEM survey";<br />
</script><br />
</body><br />
</html></div>Mlowerhttp://2010.igem.org/Template:TemplateBottomTemplate:TemplateBottom2010-10-27T23:47:07Z<p>Mlower: </p>
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</html></div>Mlowerhttp://2010.igem.org/Team:Warsaw/AttributionTeam:Warsaw/Attribution2010-10-27T23:34:43Z<p>Mlower: </p>
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<br />
<h2>Author Attribution</h2><br />
<br />
<br />
<p>Because we had ambitious project and very little time we adopted a<br />
shift based work system in the wet lab. Some team members used to come<br />
early in the morning and after dinner their experiments were passed to<br />
others, who like working late. Our dry lab worked whenever they could.</p><br />
<br />
<h3>Attributions by project:</h3><br />
<br />
<div class="note">1.RBS Measurement</div><br />
<br />
<p>20 constructs needed for this experiment were cloned by: Kasia Grześ,<br />
Ania Puławska, Ania Safray, Cherry Moreno, Milena Bażlekowa, Anna Olchowik.<br />
The lab work was supervised by Jarek Pankowski and Kasia Grześ.<br />
Kasia's skills and patience were crucial for finishing this stage of<br />
the project.</p><br />
<br />
<p>Parts' characterization using FACS measurements were conducted by<br />
Cherry Moreno under the supervision of Michał Lower with some help<br />
from other team members.</p><br />
<p>Parts' characterization using qPCR was performed by Anna Olchowik And<br />
Michał Lower. We'd like also thank Piotr Przanowski from Necki<br />
Institute, who allowed us access qPCR machine in his lab and advised<br />
us about experimental setup.</p><br />
<br />
<div class="note">2.Kill Switch(MinC)</div><br />
<p>This was Jarek Pankowski's idea.</p> <p>Cloned and tested mainly by Kuba<br />
Piątkowski and Jarek Pankowski with a some help from other team<br />
members.</p><br />
<br />
<div class="note">3.Bacto DHL</div><br />
<br />
<p>Constructs containing invasin and listeriolysin were cloned by Marta<br />
Błaszkiewicz and Joanna Leszczyńska under Michał Lower supervision. </p><br />
<p>The<br />
invasion experiments followed by flow cytometry measurements were<br />
performed by Kamil Koper, Anna Olchowik, Michał Lower and Cherry<br />
Moreno. </p><br />
<br />
<p>Microscopic observations were mainly conducted by Dominik<br />
Cysewski and Michał Lower.</p><br />
<p>Dominik's camera is also an important team member, without her we<br />
wouldn't have any pictures.</p><br />
<p>RFC that would help other teams measure cell invasion in the future<br />
was prepared by Anna Olchowik and Michał Lower.</p><br />
<br />
<div class="note">4.Human Practices</div><br />
<p>Ania Puławska, Cherry Moreno, Dominik Cysewski, Joanna Leszcyńska,<br />
Marta Błaszkiewicz, and Milena Bażlekowa prepared the presentation,<br />
went to local high schools and gave lectures about synthetic biology and<br />
iGEM competition. Moreover they prepared a questionnaire for students.</p><br />
<br />
<p>The whole team did brainstorming about<br />
Biobrick ownership. Michał came up with the idea of licensing the<br />
Biobricks like computer programs using creative commons license. Later<br />
with help of Ania Olchowik it was shaped to form of RFC document.</p><br />
<div id="humana"></div><br />
<br />
<div class="note">5. Software</div> <p>Was designed and implemented by Albert Bogdanowicz and<br />
Marta Błaszkiewicz under Michał's Lower supervision.</p><br />
<br />
<div class="note">6. Wiki</div> <p>Team's webpage draft has been coded by Anna Olchowik and<br />
Michał Lower, the final webpage has been recoded by a specialist in the<br />
field - Marta Błaszkiewicz.</p> <p>It wouldn't look nice without Ania<br />
Puławska hand-made pictures. All members of the team contributed to our<br />
wiki.</p><br />
<br />
<div class="note">7. Fundrising</div> <p>Michał Lower, Dominik Cysewski and Anna Olchowik<br />
organized funding.</p><br />
<p> Dominik's efforts need additional acknowledgment<br />
because during the summer he acted as the team's manager and<br />
fundriser. Thanks to him our team have obtained patronage of Polish<br />
Minister of Science.</p><br />
<br />
<br />
<br />
</html> <br />
<br />
{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Template:LowerTabsTeamTemplate:LowerTabsTeam2010-10-27T22:14:44Z<p>Mlower: </p>
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<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
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<br />
<ul id="HPLower" style="display:none;"> <br />
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<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
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<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1">Idea</a></li><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Background">Background</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
</ul><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Stage2">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Results">Results</a></li><br />
<br />
</ul><br />
<ul id="Stage3Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Design">Background and Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
<br />
</ul><br />
<ul id="Stage4Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
</ul><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Parts">Parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
</ul><br />
<br />
</ul><br />
<br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software/Program">See Biobrick Manager</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Acknowledgemets">Acknowledgemets</a></li><br />
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<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
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</ul><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
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<br />
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<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1">Idea</a></li><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Background">Background</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Stage2">Idea</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Results">Results</a></li><br />
<br />
</ul><br />
<ul id="Stage3Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Design">Background and Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
<br />
</ul><br />
<ul id="Stage4Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
</ul><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Parts">Parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
</ul><br />
<br />
</ul><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software/Program">See Biobrick Manager</a></li><br />
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<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
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<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
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<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
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__TOC__<br />
<br />
==Standardization==<br />
Follownig text is a brief version of [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_60:_Open_licensing_of_BioBrick_parts RFC 60 standard]. Draft version of RFC 60 text can be found [https://static.igem.org/mediawiki/2010/4/4e/RFC60_licensing_draft.pdf here]<br />
<br />
==Introduction==<br />
<html><br />
<img src="https://static.igem.org/mediawiki/2010/2/28/Licencja.jpg" align="right" width="200px"><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
Currently licensing of community-created biological parts is very<br />
similar to the situation on software scene before General Public<br />
License (aka. GNU GPL) was created. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
There are many great parts and devices created by the community<br />
members and released freely to the community. In addition there is<br />
very little awareness of keeping community-developed parts and their<br />
derivatives free-as-in-freedom, meaning that the work is available<br />
free of charge but can also be freely accessed and used by anyone to<br />
create new parts that still remain in the Public Domain. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
This can lead to the situation when work which was intended to<br />
remain in the Public Domain would be patented as a part of some<br />
project and the results of this project would not be returned to the<br />
community. Moreover this allows the situation in which it's legal to<br />
remove original author attribution information from parts containing<br />
elements developed by others.</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
<BR><br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
We recommend that all biological parts released to the community<br />
should be licensed in a way that ensures that the original part as<br />
well as all other parts using it can be obtained, used and modified<br />
without any limitations. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
We also recommend that single licensing scheme based on Creative<br />
BioCommons licenses (see below), a Creative Commons derivative<br />
applied to biological parts, should be adopted by synthetic biology<br />
community. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
This will ensure that original author attribution information has to<br />
be included when a new part based on the work of others is developed.<br />
Moreover Creative BioCommons license is inherent, meaning that all<br />
derivatives of the work (ie. BioBrick part) licensed under BioCommons<br />
have to be licensed alike. In simple words this gives a legal<br />
warranty that all You create with intention to share with others will<br />
remain in the community. Additionally it ensures acknowledgment of<br />
Your authorship.</P><br />
</html><br />
<br />
==BioCommons in plain words ==<br />
<br />
For all those who are not lawyers here is simple explanation of CreativeBiocommons features.<br />
<br />
Both BioCommons and BioCommons non-commercial are based on Creative Commons license texts with definitions updated to apply to biological parts.<br />
<br />
===Creative BioCommons license allows===<br />
*[http://www.gnu.org/philosophy/free-sw.html free-as-in-freedom] usage, modification and distribution of biological parts covered by this license<br />
*BioCommons allows commercial usage of covered materials, BioCommons non-commercial forbids it<br />
<br />
===as long as===<br />
*parts that are build upon elements licensed under BioCommons are licensed alike (licensing BioCommons is perpetual)<br />
*original information about authors of all subparts is retained<br />
*information about parts is distributed with link to BioCommons text<br />
<br />
===it is forbidden to===<br />
*change licensing of parts built upon BioCommons licensed parts to less open one. License must remain Creative Commons compatible (currently Biocommons or BioCommons non-commenrcial)<br />
*remove information about authors<br />
*refuse others access to parts built upon Biocommmons licensed parts<br />
<br />
==Creative BioCommons legal code==<br />
<html><br />
<H3 LANG="en-US" CLASS="western" STYLE="line-height: 150%"><EM>License</EM></H3><br />
<P LANG="en-US" CLASS="western">THE WORK (AS DEFINED BELOW) IS<br />
PROVIDED UNDER THE TERMS OF THIS CREATIVE BIOCOMMONS PUBLIC LICENSE<br />
(&quot;CBCPL&quot; OR &quot;LICENSE&quot;). THE WORK IS PROTECTED BY<br />
COPYRIGHT AND/OR OTHER APPLICABLE LAW. ANY USE OF THE WORK OTHER THAN<br />
AS AUTHORIZED UNDER THIS LICENSE OR COPYRIGHT LAW IS PROHIBITED.</P><br />
<P LANG="en-US" CLASS="western">BY EXERCISING ANY RIGHTS TO THE WORK<br />
PROVIDED HERE, YOU ACCEPT AND AGREE TO BE BOUND BY THE TERMS OF THIS<br />
LICENSE. TO THE EXTENT THIS LICENSE MAY BE CONSIDERED TO BE A<br />
CONTRACT, THE LICENSOR GRANTS YOU THE RIGHTS CONTAINED HERE IN<br />
CONSIDERATION OF YOUR ACCEPTANCE OF SUCH TERMS AND CONDITIONS.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>1. Definitions</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Adaptation&quot;</STRONG><br />
means a work based upon the Work, or upon the Work and other<br />
pre-existing works, such as a adaptation, derivative work or other<br />
alterations of a work or any other form in which the Work may be<br />
recast, transformed, or adapted including in any form recognizably<br />
derived from the original. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Collection&quot;</STRONG><br />
means a collection of nucleotide or protein sequences, either in<br />
form of physical DNA or sequence alone, which, by reason of the<br />
selection and arrangement of their contents, constitute intellectual<br />
creations, in which the Work is included in its entirety in<br />
unmodified form along with one or more other contributions, each<br />
constituting separate and independent works in themselves, which<br />
together are assembled into a collective whole. A work that<br />
constitutes a Collection will not be considered an Adaptation (as<br />
defined below) for the purposes of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Creative<br />
Commons Compatible License&quot;</STRONG> means a license that is<br />
listed at http://creativecommons.org/compatiblelicenses that has<br />
been approved by Creative Commons as being essentially equivalent to<br />
this License, including, at a minimum, because that license: (i)<br />
contains terms that have the same purpose, meaning and effect as the<br />
License Elements of this License; and, (ii) explicitly permits the<br />
relicensing of adaptations of works made available under that<br />
license under this License or a Creative Commons jurisdiction<br />
license with the same License Elements as this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Distribute&quot;</STRONG><br />
means to make available to the public the original and copies of the<br />
Work or Adaptation, as appropriate, through sale or other transfer<br />
of ownership. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;License<br />
Elements&quot;</STRONG> means the following high-level license<br />
attributes as selected by Licensor and indicated in the title of<br />
this License: Attribution. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Licensor&quot;</STRONG><br />
means the individual, individuals, entity or entities that offer(s)<br />
the Work under the terms of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Original<br />
Author&quot;</STRONG> means the individual, individuals, entity or<br />
entities who created the Work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Work&quot;</STRONG><br />
means biologically meaningful sequence either in form of DNA,<br />
protein or information about DNA or protein sequence offered under<br />
the terms of this License including without limitation any<br />
production in the literary, scientific and artistic domain, whatever<br />
may be the mode or form of its expression including digital form,<br />
such as a book, and other writing; a lecture, address, sermon or<br />
other work of the same nature; an illustration, plan, sketch or<br />
three-dimensional work relative to science; a compilation of data to<br />
the extent it is protected as a copyrightable work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;You&quot;</STRONG><br />
means an individual or entity exercising rights under this License<br />
who has not previously violated the terms of this License with<br />
respect to the Work, or who has received express permission from the<br />
Licensor to exercise rights under this License despite a previous<br />
violation. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Publicly<br />
Perform&quot;</STRONG> means to perform public recitations of the<br />
Work and to communicate to the public those public recitations, by<br />
any means or process, including by wire or wireless means or public<br />
digital performances; to make available to the public Works in such<br />
a way that members of the public may access these Works from a place<br />
and at a place individually chosen by them; to perform the Work to<br />
the public by any means or process and the communication to the<br />
public of the performances of the Work, including by public digital<br />
performance; to broadcast and rebroadcast the Work by any means. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>&quot;Reproduce&quot;</STRONG><br />
means to make copies of the Work by any means including in digital<br />
form or via other electronic medium. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>2. Fair Dealing Rights.</STRONG><br />
Nothing in this License is intended to reduce, limit, or restrict any<br />
uses free from copyright or rights arising from limitations or<br />
exceptions that are provided for in connection with the copyright<br />
protection under copyright law or other applicable laws.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>3. License Grant.</STRONG><br />
Subject to the terms and conditions of this License, Licensor hereby<br />
grants You a worldwide, royalty-free, non-exclusive, perpetual (for<br />
the duration of the applicable copyright) license to exercise the<br />
rights in the Work as stated below:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Reproduce the Work, to incorporate the Work into one or more<br />
Collections, and to Reproduce the Work as incorporated in the<br />
Collections; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
create and Reproduce Adaptations provided that any such Adaptation,<br />
including any translation in any medium, takes reasonable steps to<br />
clearly label, demarcate or otherwise identify that changes were<br />
made to the original Work. For example, a translation could be<br />
marked &quot;The original work was translated from English to<br />
Spanish,&quot; or a modification could indicate &quot;The original<br />
work has been modified.&quot;; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform the Work including as incorporated<br />
in Collections; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform Adaptations. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">For the avoidance of doubt:</P><br />
<OL TYPE=i><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Non-waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme cannot be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme can be waived, the Licensor waives the<br />
exclusive right to collect such royalties for any exercise by You<br />
of the rights granted under this License; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>Voluntary License<br />
Schemes</STRONG>. The Licensor waives the right to collect<br />
royalties, whether individually or, in the event that the Licensor<br />
is a member of a collecting society that administers voluntary<br />
licensing schemes, via that society, from any exercise by You of<br />
the rights granted under this License. <br />
</P><br />
</OL><br />
</OL><br />
<P LANG="en-US" CLASS="western">The above rights may be exercised in<br />
all media and formats whether now known or hereafter devised. The<br />
above rights include the right to make such modifications as are<br />
technically necessary to exercise the rights in other media and<br />
formats. Subject to Section 8(f), all rights not expressly granted by<br />
Licensor are hereby reserved.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>4. Restrictions.</STRONG> The<br />
license granted in Section 3 above is expressly made subject to and<br />
limited by the following restrictions:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform the Work only under the terms of<br />
this License. You must include a copy of, or the Uniform Resource<br />
Identifier (URI) for, this License with every copy of the Work You<br />
Distribute or Publicly Perform. You may not offer or impose any<br />
terms on the Work that restrict the terms of this License or the<br />
ability of the recipient of the Work to exercise the rights granted<br />
to that recipient under the terms of the License. You may not<br />
sublicense the Work. You must keep intact all notices that refer to<br />
this License and to the disclaimer of warranties with every copy of<br />
the Work You Distribute or Publicly Perform. When You Distribute or<br />
Publicly Perform the Work, You may not impose any effective<br />
technological measures on the Work that restrict the ability of a<br />
recipient of the Work from You to exercise the rights granted to<br />
that recipient under the terms of the License. This Section 4(a)<br />
applies to the Work as incorporated in a Collection, but this does<br />
not require the Collection apart from the Work itself to be made<br />
subject to the terms of this License. If You create a Collection,<br />
upon notice from any Licensor You must, to the extent practicable,<br />
remove from the Collection any credit as required by Section 4(c),<br />
as requested. If You create an Adaptation, upon notice from any<br />
Licensor You must, to the extent practicable, remove from the<br />
Adaptation any credit as required by Section 4(c), as requested. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform an Adaptation only under the<br />
terms of: (i) this License; (ii) a later version of this License<br />
with the same License Elements as this License; (iii) a Creative<br />
Commons Compatible License. If you license the Adaptation under one<br />
of the licenses mentioned in (iii), you must comply with the terms<br />
of that license. If you license the Adaptation under the terms of<br />
any of the licenses mentioned in (i), (ii) or (iii) (the &quot;Applicable<br />
License&quot;), you must comply with the terms of the Applicable<br />
License generally and the following provisions: (I) You must include<br />
a copy of, or the URI for, the Applicable License with every copy of<br />
each Adaptation You Distribute or Publicly Perform; (II) You may not<br />
offer or impose any terms on the Adaptation that restrict the terms<br />
of the Applicable License or the ability of the recipient of the<br />
Adaptation to exercise the rights granted to that recipient under<br />
the terms of the Applicable License; (III) You must keep intact all<br />
notices that refer to the Applicable License and to the disclaimer<br />
of warranties with every copy of the Work as included in the<br />
Adaptation You Distribute or Publicly Perform; (IV) when You<br />
Distribute or Publicly Perform the Adaptation, You may not impose<br />
any effective technological measures on the Adaptation that restrict<br />
the ability of a recipient of the Adaptation from You to exercise<br />
the rights granted to that recipient under the terms of the<br />
Applicable License. This Section 4(b) applies to the Adaptation as<br />
incorporated in a Collection, but this does not require the<br />
Collection apart from the Adaptation itself to be made subject to<br />
the terms of the Applicable License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
You Distribute, or Publicly Perform the Work or any Adaptations or<br />
Collections, You must, unless a request has been made pursuant to<br />
Section 4(a), keep intact all copyright notices for the Work and<br />
provide, reasonable to the medium or means You are utilizing: (i)<br />
the name of the Original Author (or pseudonym, if applicable) if<br />
supplied, and/or if the Original Author and/or Licensor designate<br />
another party or parties (e.g., a sponsor institute, publishing<br />
entity, journal) for attribution (&quot;Attribution Parties&quot;)<br />
in Licensor's copyright notice, terms of service or by other<br />
reasonable means, the name of such party or parties; (ii) the title<br />
of the Work if supplied; (iii) to the extent reasonably practicable,<br />
the URI, if any, that Licensor specifies to be associated with the<br />
Work, unless such URI does not refer to the copyright notice or<br />
licensing information for the Work; and (iv) , consistent with<br />
Ssection 3(b), in the case of an Adaptation, a credit identifying<br />
the use of the Work in the Adaptation (e.g., &quot;French<br />
translation of the Work by Original Author,&quot; or &quot;Screenplay<br />
based on original Work by Original Author&quot;). The credit<br />
required by this Section 4(c) may be implemented in any reasonable<br />
manner; provided, however, that in the case of a Adaptation or<br />
Collection, at a minimum such credit will appear, if a credit for<br />
all contributing authors of the Adaptation or Collection appears,<br />
then as part of these credits and in a manner at least as prominent<br />
as the credits for the other contributing authors. For the avoidance<br />
of doubt, You may only use the credit required by this Section for<br />
the purpose of attribution in the manner set out above and, by<br />
exercising Your rights under this License, You may not implicitly or<br />
explicitly assert or imply any connection with, sponsorship or<br />
endorsement by the Original Author, Licensor and/or Attribution<br />
Parties, as appropriate, of You or Your use of the Work, without the<br />
separate, express prior written permission of the Original Author,<br />
Licensor and/or Attribution Parties. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Except as otherwise agreed in<br />
writing by the Licensor or as may be otherwise permitted by<br />
applicable law, if You Reproduce, Distribute or Publicly Perform the<br />
Work either by itself or as part of any Adaptations or Collections,<br />
You must not distort, mutilate, modify or take other derogatory<br />
action in relation to the Work which would be prejudicial to the<br />
Original Author's honor or reputation. Licensor agrees that in those<br />
jurisdictions (e.g. Japan), in which any exercise of the right<br />
granted in Section 3(b) of this License (the right to make<br />
Adaptations) would be deemed to be a distortion, mutilation,<br />
modification or other derogatory action prejudicial to the Original<br />
Author's honor and reputation, the Licensor will waive or not<br />
assert, as appropriate, this Section, to the fullest extent<br />
permitted by the applicable national law, to enable You to<br />
reasonably exercise Your right under Section 3(b) of this License<br />
(right to make Adaptations) but not otherwise. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>5. Representations,<br />
Warranties and Disclaimer</STRONG></P><br />
<P LANG="en-US" CLASS="western">UNLESS OTHERWISE MUTUALLY AGREED TO<br />
BY THE PARTIES IN WRITING, LICENSOR OFFERS THE WORK AS-IS AND MAKES<br />
NO REPRESENTATIONS OR WARRANTIES OF ANY KIND CONCERNING THE WORK,<br />
EXPRESS, IMPLIED, STATUTORY OR OTHERWISE, INCLUDING, WITHOUT<br />
LIMITATION, WARRANTIES OF TITLE, MERCHANTIBILITY, FITNESS FOR A<br />
PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR<br />
OTHER DEFECTS, ACCURACY, OR THE PRESENCE OF ABSENCE OF ERRORS,<br />
WHETHER OR NOT DISCOVERABLE. SOME JURISDICTIONS DO NOT ALLOW THE<br />
EXCLUSION OF IMPLIED WARRANTIES, SO SUCH EXCLUSION MAY NOT APPLY TO<br />
YOU.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>6. Limitation on Liability.</STRONG><br />
EXCEPT TO THE EXTENT REQUIRED BY APPLICABLE LAW, IN NO EVENT WILL<br />
LICENSOR BE LIABLE TO YOU ON ANY LEGAL THEORY FOR ANY SPECIAL,<br />
INCIDENTAL, CONSEQUENTIAL, PUNITIVE OR EXEMPLARY DAMAGES ARISING OUT<br />
OF THIS LICENSE OR THE USE OF THE WORK, EVEN IF LICENSOR HAS BEEN<br />
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>7. Termination</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License and the rights granted hereunder will terminate<br />
automatically upon any breach by You of the terms of this License.<br />
Individuals or entities who have received Adaptations or Collections<br />
from You under this License, however, will not have their licenses<br />
terminated provided such individuals or entities remain in full<br />
compliance with those licenses. Sections 1, 2, 5, 6, 7, and 8 will<br />
survive any termination of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Subject to the above terms and<br />
conditions, the license granted here is perpetual (for the duration<br />
of the applicable copyright in the Work). Notwithstanding the above,<br />
Licensor reserves the right to release the Work under different<br />
license terms or to stop distributing the Work at any time;<br />
provided, however that any such election will not serve to withdraw<br />
this License (or any other license that has been, or is required to<br />
be, granted under the terms of this License), and this License will<br />
continue in full force and effect unless terminated as stated above.<br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>8. Miscellaneous</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform the Work or a Collection,<br />
the Licensor offers to the recipient a license to the Work on the<br />
same terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform an Adaptation, Licensor<br />
offers to the recipient a license to the original Work on the same<br />
terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
any provision of this License is invalid or unenforceable under<br />
applicable law, it shall not affect the validity or enforceability<br />
of the remainder of the terms of this License, and without further<br />
action by the parties to this agreement, such provision shall be<br />
reformed to the minimum extent necessary to make such provision<br />
valid and enforceable. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">No<br />
term or provision of this License shall be deemed waived and no<br />
breach consented to unless such waiver or consent shall be in<br />
writing and signed by the party to be charged with such waiver or<br />
consent. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License constitutes the entire agreement between the parties with<br />
respect to the Work licensed here. There are no understandings,<br />
agreements or representations with respect to the Work not specified<br />
here. Licensor shall not be bound by any additional provisions that<br />
may appear in any communication from You. This License may not be<br />
modified without the mutual written agreement of the Licensor and<br />
You. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">The rights granted under, and<br />
the subject matter referenced, in this License were drafted<br />
utilizing the terminology of the Berne Convention for the Protection<br />
of Literary and Artistic Works (as amended on September 28, 1979),<br />
the Rome Convention of 1961, the WIPO Copyright Treaty of 1996, the<br />
WIPO Performances and Phonograms Treaty of 1996 and the Universal<br />
Copyright Convention (as revised on July 24, 1971). These rights and<br />
subject matter take effect in the relevant jurisdiction in which the<br />
License terms are sought to be enforced according to the<br />
corresponding provisions of the implementation of those treaty<br />
provisions in the applicable national law. If the standard suite of<br />
rights granted under applicable copyright law includes additional<br />
rights not granted under this License, such additional rights are<br />
deemed to be included in the License; this License is not intended<br />
to restrict the license of any rights under applicable law. <br />
</P><br />
</OL><br />
</html><br />
<br />
<br />
==Creative BioCommons NonCommercial legal code==<br />
<html><br />
<H3 LANG="en-US" CLASS="western" STYLE="line-height: 150%"><EM>License</EM></H3><br />
<P LANG="en-US" CLASS="western">THE WORK (AS DEFINED BELOW) IS<br />
PROVIDED UNDER THE TERMS OF THIS CREATIVE COMMONS PUBLIC LICENSE<br />
(&quot;CCPL&quot; OR &quot;LICENSE&quot;). THE WORK IS PROTECTED BY<br />
COPYRIGHT AND/OR OTHER APPLICABLE LAW. ANY USE OF THE WORK OTHER THAN<br />
AS AUTHORIZED UNDER THIS LICENSE OR COPYRIGHT LAW IS PROHIBITED.</P><br />
<P LANG="en-US" CLASS="western">BY EXERCISING ANY RIGHTS TO THE WORK<br />
PROVIDED HERE, YOU ACCEPT AND AGREE TO BE BOUND BY THE TERMS OF THIS<br />
LICENSE. TO THE EXTENT THIS LICENSE MAY BE CONSIDERED TO BE A<br />
CONTRACT, THE LICENSOR GRANTS YOU THE RIGHTS CONTAINED HERE IN<br />
CONSIDERATION OF YOUR ACCEPTANCE OF SUCH TERMS AND CONDITIONS.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>1. Definitions</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Adaptation&quot;</STRONG><br />
means a work based upon the Work, or upon the Work and other<br />
pre-existing works, such as a adaptation, derivative work or other<br />
alterations of a work or any other form in which the Work may be<br />
recast, transformed, or adapted including in any form recognizably<br />
derived from the original. <br />
</P><br />
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</html><br />
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<br />
{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Stage1/PromMeasTeam:Warsaw/Stage1/PromMeas2010-10-27T21:25:43Z<p>Mlower: </p>
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</script><br />
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<h2>Promoter measurement</h2><br />
<br />
<h3>Standardization</h3><br />
All measurements were done according to <a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_58:_Absolute_measurement_of_bacterial_promoter_strength_in_cell-free_system_by_qPCR">RFC 58 standard</a>. Draft version of RFC document can be found <a href="https://static.igem.org/mediawiki/2010/b/b8/RFC58_draft.pdf">here</a>.<br />
<h3>Experimental setup</h3><br />
<div class="note">Measured parts</div><br />
<p>We have measured <a href="http://partsregistry.org/Part:BBa_J23100">J23100</a> and <a href="http://partsregistry.org/Part:BBa_I719005">I719005 encoding pT7</a>.</p> <h4>J23100 with our reference RBS has RNA synthesis rate of 13,1pg RNA/minute/ug substrate DNA. It equals 2,80 microPoPS</h4><br />
<h4>pT7 with our reference RBS has RNA synthesis rate of 41,8pg RNA/minute/ug substrate DNA. It equals 8,92 microPoPS </h4><br />
<br />
<div class="note">Measurement constructs</div><br />
<p>We have used <a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> as our reference RBS and <a href="http://partsregistry.org/Part:BBa_E0040">E0040</a> GFP as a reporter:<br />
<br />
</html><br />
<br />
<partinfo>BBa_K299009 DeepComponents</partinfo> <br />
<br />
<partinfo>BBa_K299024 DeepComponents</partinfo> <br />
<br />
<html><br />
<div class="note">Results</div><br />
<p>Measured promoter strengths are as follows:</p><br />
pT7 41,8pg RNA/minute/ug substrate DNA<br />
<br> J23100 13,1pg RNA/minute/ug substrate DNA<br><br><br />
<p>It means that from 1 ug of the DNA containing pT7 regulated reporter DNA polymerase made around 41 pg of RNA in a one minute. <b>Is it a good unit for promoter activity?</b> What happens when we input into our cell-free system DNA with the same BioBrick, but in a different vector? The amount of DNA changes, but it doesn't change the number of available promoters. Therefore we have to change those units to something reasonable - like <b>number of RNA molecules made from 1 DNA molecule in a unit of time</b>. Sounds like <a href="http://partsregistry.org/wiki/index.php/Part_Types:Measurement_Systems">PoPs (polymerase per second) per DNA copy</a></p><br />
<br />
<br />
<div class="note">Converting measured values into PoPs per molecule of substrate DNA</div><br><br />
</html><br />
<partinfo>BBa_K299009 DeepComponents</partinfo><html> K299009 is 918 bp long</html><br />
<br />
<partinfo>BBa_K299024 DeepComponents</partinfo><html> K299024 is 906 bp long<br><br />
</html><partinfo>pSB1A2 DeepComponents</partinfo><html> Vector backbone has 2079 bp<br />
<br><br />
<br />
<h3>Example calcultaions for BBa_K299024:</h3><br />
1) Approximated molecular weight of dsDNA (circular plasmid) = # nucleotides x 607.4 gram/mole. <br><br />
BBa_K299024 in pSB1A2 is 2985 bp long.<br><br />
Approximated molecular weight of K299024 in pSB1A2 is 1813089 gram/mole.<br><br />
in 0.5 ug there is 2,76E-013 moles of BBa_K299024 in pSB1A2 DNA (divide 0.5 ug by construct's molar mass)<br />
<br />
<br><br />
2) Promoter is not transcribed therefore we assume that only the RBS sequence and gene sequence are transcribed. In both constructs RBS BBa_B0034 was used and the same GFP mut3b with B0015 terminator therefore the same RNA is produced from two measured promoters.<br><br />
Approximated molecular weight of ssRNA (mRNA transcript is = # nucleotides x 320.5 + 159.0 (takes into account the 5'triphosphate)<br />
mRNA is single stranded and 1 Mole of single stranded RNA is 283160,5g. <br />
<br><br />
3) Measured strength of BBa_K299024 is 4,18E-011 g RNA/min/ug DNA, that's 2,46E-018 mol RNA/sec/ug DNA (divide by molar mass of mRNA to convert from g to moles and then divide by 60 to convert from minutes to seconds)<br />
<br><br />
4) Strength of BBa_K299024 expressed in PoPS is 8,92E-006 (divide the result from point 3) by amount of moles of DNA construct in 0.5 ug)<br />
<br><br />
Same calculations for BBa_K299009 will give 2,80E-006 PoPS<br />
<br />
<br><br />
<div class="note">Where did we get the experimental values from?</div><br><br />
<p> We expressed known amount if DNA in a cell-free expression system and measured RNA content over time using RTq-PCR. Simply we put the DNA into the tube with the cell-free expression system and collected samples of the mixture every 15 minutes. Then we did the reverse transcription to be able to perform a q-PCR that gives information on the amount of starting material. <br><br />
Figure 1 shows you the dynamic performance of J23100 and T7 promoters - the amount of RNA versus time. Figure 2 show amplification curves that we got after RTq-PCR. <br />
<br />
<img src="https://static.igem.org/mediawiki/2010/f/f3/J23_i_T7_dynamic.png"><br />
<p>Fig 1. Dynamic performance of J23100 and pT7.</p><br />
<br> <br />
<img src="https://static.igem.org/mediawiki/2010/1/1c/Amplification_curve_T7.png" width="50%"><img src="https://static.igem.org/mediawiki/2010/c/c5/Amplification_curve_J23.png" width="50%"><br />
Fig 2. The amplification curves that were obtained after RTq-PCR seperately for J23100 and T7 containing construct.<br />
Different colours indicate that samples were collected at different times: 15, 30. 45, etc. minutes after DNA addition. Dark blue is amplification curve for sample after 15 minutes and light blue is after 90, as you can see there is an increase in the RNA content.</p><br />
<br />
<br><br />
<br><br />
<div class="note">Methodology</div><br />
<p>All measurements were conducted in cell-free system which allowed us simple and precise determination of GFP encoding mRNA. The amount of mRNA was determined using quantitative reverse transcriptase realtime PCR (qRT-PCR). We have done absolute quantification using cDNA standard curve to convert delta-Ct units to RNA concentration.</p><br />
<br />
<p> We have used following protocol:</p><br />
<ol><br />
<li>All reagents and substrates were RNase free. Experiments were conducted in RNase-free environment.</li><br />
<li>0.5 ug of DNA encoding tested construct was added to 50 ul of cell-free expression master mix containing 350 units of human placental RNase inhibitor.</li><br />
<li>Samples were incubated at 37<sup>o</sup>C with shaking at 800 RPM</li><br />
<li>Every 15 minutes 5ul of reaction mixture was collected. Reaction was stopped by freezing at -20. Samples were kept frozen until reverse transcription.</li><br />
<li>Subsamples were being collected untill reaction have reached steady state (typically 120 minutes).</li><br />
<li>After obtaining all RNA samples DNAse treatment was performed as follows: 5 ul of sample was supplemented with 1ul of 10x DNAse buffer, 3 ul of RNase-free water and 1ul of RNase-free DNase I from Fermentas</li><br />
<li>Samples were incubated at 37<sup>o</sup>C for 30 minutes. After that time 1ul of EDTA was added to each sample.</li><br />
<li>DNase was inactivated by heating in 65<sup>o</sup>C for 10 minutes.</li><br />
<li>DNase treated RNA samples were divided in two. One half was used as a substrate for reverse transcription. The other halves were mixed together and used in -RT control reaction.</li><br />
<li>Reverse transcription was performed using Maxima First Strand cDNA synthesis kit form Fermentas using manufacturer's instructions. Gene specific primer GFPqPCRr (TCGAAAGGGCAGATTGTG) was used.</li><br />
<li>cDNA was diluted 50x and 1ul was used for qRT-PCR reaction. SYBR/ROX qPCR HotStart 2x Master Mix for Fermentas was used to perform the reaction with the following primers: GFPqPCRf (GATGACGGGAACTACAAGAC) and GFPqPCRr (TCGAAAGGGCAGATTGTG). ABI 7500 qPCR system was used. PCR program: 95<sup>o</sup>C for 10 minutes followed with 40 cycles of 95<sup>o</sup>C for 15s, 55<sup>o</sup>C for 30 s, 72<sup>o</sup>C for 40s. Reaction specificity was confirmed using melting curve analysis.</li><br />
</ol><br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/File:RFC58_draft.pdfFile:RFC58 draft.pdf2010-10-27T21:23:36Z<p>Mlower: </p>
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<div></div>Mlowerhttp://2010.igem.org/Team:Warsaw/HP/LicensingTeam:Warsaw/HP/Licensing2010-10-27T21:18:19Z<p>Mlower: /* Creative BioCommons license allows */</p>
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__TOC__<br />
<br />
==Introduction==<br />
<html><br />
<img src="https://static.igem.org/mediawiki/2010/2/28/Licencja.jpg" align="right" width="200px"><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
Currently licensing of community-created biological parts is very<br />
similar to the situation on software scene before General Public<br />
License (aka. GNU GPL) was created. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
There are many great parts and devices created by the community<br />
members and released freely to the community. In addition there is<br />
very little awareness of keeping community-developed parts and their<br />
derivatives free-as-in-freedom, meaning that the work is available<br />
free of charge but can also be freely accessed and used by anyone to<br />
create new parts that still remain in the Public Domain. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
This can lead to the situation when work which was intended to<br />
remain in the Public Domain would be patented as a part of some<br />
project and the results of this project would not be returned to the<br />
community. Moreover this allows the situation in which it's legal to<br />
remove original author attribution information from parts containing<br />
elements developed by others.</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
<BR><br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
We recommend that all biological parts released to the community<br />
should be licensed in a way that ensures that the original part as<br />
well as all other parts using it can be obtained, used and modified<br />
without any limitations. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
We also recommend that single licensing scheme based on Creative<br />
BioCommons licenses (see below), a Creative Commons derivative<br />
applied to biological parts, should be adopted by synthetic biology<br />
community. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
This will ensure that original author attribution information has to<br />
be included when a new part based on the work of others is developed.<br />
Moreover Creative BioCommons license is inherent, meaning that all<br />
derivatives of the work (ie. BioBrick part) licensed under BioCommons<br />
have to be licensed alike. In simple words this gives a legal<br />
warranty that all You create with intention to share with others will<br />
remain in the community. Additionally it ensures acknowledgment of<br />
Your authorship.</P><br />
</html><br />
<br />
==BioCommons in plain words ==<br />
<br />
For all those who are not lawyers here is simple explanation of CreativeBiocommons features.<br />
<br />
Both BioCommons and BioCommons non-commercial are based on Creative Commons license texts with definitions updated to apply to biological parts.<br />
<br />
===Creative BioCommons license allows===<br />
*[http://www.gnu.org/philosophy/free-sw.html free-as-in-freedom] usage, modification and distribution of biological parts covered by this license<br />
*BioCommons allows commercial usage of covered materials, BioCommons non-commercial forbids it<br />
<br />
===as long as===<br />
*parts that are build upon elements licensed under BioCommons are licensed alike (licensing BioCommons is perpetual)<br />
*original information about authors of all subparts is retained<br />
*information about parts is distributed with link to BioCommons text<br />
<br />
===it is forbidden to===<br />
*change licensing of parts built upon BioCommons licensed parts to less open one. License must remain Creative Commons compatible (currently Biocommons or BioCommons non-commenrcial)<br />
*remove information about authors<br />
*refuse others access to parts built upon Biocommmons licensed parts<br />
<br />
==Creative BioCommons legal code==<br />
<html><br />
<H3 LANG="en-US" CLASS="western" STYLE="line-height: 150%"><EM>License</EM></H3><br />
<P LANG="en-US" CLASS="western">THE WORK (AS DEFINED BELOW) IS<br />
PROVIDED UNDER THE TERMS OF THIS CREATIVE BIOCOMMONS PUBLIC LICENSE<br />
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COPYRIGHT AND/OR OTHER APPLICABLE LAW. ANY USE OF THE WORK OTHER THAN<br />
AS AUTHORIZED UNDER THIS LICENSE OR COPYRIGHT LAW IS PROHIBITED.</P><br />
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PROVIDED HERE, YOU ACCEPT AND AGREE TO BE BOUND BY THE TERMS OF THIS<br />
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CONTRACT, THE LICENSOR GRANTS YOU THE RIGHTS CONTAINED HERE IN<br />
CONSIDERATION OF YOUR ACCEPTANCE OF SUCH TERMS AND CONDITIONS.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>1. Definitions</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Adaptation&quot;</STRONG><br />
means a work based upon the Work, or upon the Work and other<br />
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<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Creative<br />
Commons Compatible License&quot;</STRONG> means a license that is<br />
listed at http://creativecommons.org/compatiblelicenses that has<br />
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<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Distribute&quot;</STRONG><br />
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Work or Adaptation, as appropriate, through sale or other transfer<br />
of ownership. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;License<br />
Elements&quot;</STRONG> means the following high-level license<br />
attributes as selected by Licensor and indicated in the title of<br />
this License: Attribution. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Licensor&quot;</STRONG><br />
means the individual, individuals, entity or entities that offer(s)<br />
the Work under the terms of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Original<br />
Author&quot;</STRONG> means the individual, individuals, entity or<br />
entities who created the Work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Work&quot;</STRONG><br />
means biologically meaningful sequence either in form of DNA,<br />
protein or information about DNA or protein sequence offered under<br />
the terms of this License including without limitation any<br />
production in the literary, scientific and artistic domain, whatever<br />
may be the mode or form of its expression including digital form,<br />
such as a book, and other writing; a lecture, address, sermon or<br />
other work of the same nature; an illustration, plan, sketch or<br />
three-dimensional work relative to science; a compilation of data to<br />
the extent it is protected as a copyrightable work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;You&quot;</STRONG><br />
means an individual or entity exercising rights under this License<br />
who has not previously violated the terms of this License with<br />
respect to the Work, or who has received express permission from the<br />
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<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Publicly<br />
Perform&quot;</STRONG> means to perform public recitations of the<br />
Work and to communicate to the public those public recitations, by<br />
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<LI><P LANG="en-US" CLASS="western"><STRONG>&quot;Reproduce&quot;</STRONG><br />
means to make copies of the Work by any means including in digital<br />
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</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>2. Fair Dealing Rights.</STRONG><br />
Nothing in this License is intended to reduce, limit, or restrict any<br />
uses free from copyright or rights arising from limitations or<br />
exceptions that are provided for in connection with the copyright<br />
protection under copyright law or other applicable laws.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>3. License Grant.</STRONG><br />
Subject to the terms and conditions of this License, Licensor hereby<br />
grants You a worldwide, royalty-free, non-exclusive, perpetual (for<br />
the duration of the applicable copyright) license to exercise the<br />
rights in the Work as stated below:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Reproduce the Work, to incorporate the Work into one or more<br />
Collections, and to Reproduce the Work as incorporated in the<br />
Collections; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
create and Reproduce Adaptations provided that any such Adaptation,<br />
including any translation in any medium, takes reasonable steps to<br />
clearly label, demarcate or otherwise identify that changes were<br />
made to the original Work. For example, a translation could be<br />
marked &quot;The original work was translated from English to<br />
Spanish,&quot; or a modification could indicate &quot;The original<br />
work has been modified.&quot;; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform the Work including as incorporated<br />
in Collections; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform Adaptations. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">For the avoidance of doubt:</P><br />
<OL TYPE=i><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Non-waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme cannot be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme can be waived, the Licensor waives the<br />
exclusive right to collect such royalties for any exercise by You<br />
of the rights granted under this License; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>Voluntary License<br />
Schemes</STRONG>. The Licensor waives the right to collect<br />
royalties, whether individually or, in the event that the Licensor<br />
is a member of a collecting society that administers voluntary<br />
licensing schemes, via that society, from any exercise by You of<br />
the rights granted under this License. <br />
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</OL><br />
</OL><br />
<P LANG="en-US" CLASS="western">The above rights may be exercised in<br />
all media and formats whether now known or hereafter devised. The<br />
above rights include the right to make such modifications as are<br />
technically necessary to exercise the rights in other media and<br />
formats. Subject to Section 8(f), all rights not expressly granted by<br />
Licensor are hereby reserved.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>4. Restrictions.</STRONG> The<br />
license granted in Section 3 above is expressly made subject to and<br />
limited by the following restrictions:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform the Work only under the terms of<br />
this License. You must include a copy of, or the Uniform Resource<br />
Identifier (URI) for, this License with every copy of the Work You<br />
Distribute or Publicly Perform. You may not offer or impose any<br />
terms on the Work that restrict the terms of this License or the<br />
ability of the recipient of the Work to exercise the rights granted<br />
to that recipient under the terms of the License. You may not<br />
sublicense the Work. You must keep intact all notices that refer to<br />
this License and to the disclaimer of warranties with every copy of<br />
the Work You Distribute or Publicly Perform. When You Distribute or<br />
Publicly Perform the Work, You may not impose any effective<br />
technological measures on the Work that restrict the ability of a<br />
recipient of the Work from You to exercise the rights granted to<br />
that recipient under the terms of the License. This Section 4(a)<br />
applies to the Work as incorporated in a Collection, but this does<br />
not require the Collection apart from the Work itself to be made<br />
subject to the terms of this License. If You create a Collection,<br />
upon notice from any Licensor You must, to the extent practicable,<br />
remove from the Collection any credit as required by Section 4(c),<br />
as requested. If You create an Adaptation, upon notice from any<br />
Licensor You must, to the extent practicable, remove from the<br />
Adaptation any credit as required by Section 4(c), as requested. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform an Adaptation only under the<br />
terms of: (i) this License; (ii) a later version of this License<br />
with the same License Elements as this License; (iii) a Creative<br />
Commons Compatible License. If you license the Adaptation under one<br />
of the licenses mentioned in (iii), you must comply with the terms<br />
of that license. If you license the Adaptation under the terms of<br />
any of the licenses mentioned in (i), (ii) or (iii) (the &quot;Applicable<br />
License&quot;), you must comply with the terms of the Applicable<br />
License generally and the following provisions: (I) You must include<br />
a copy of, or the URI for, the Applicable License with every copy of<br />
each Adaptation You Distribute or Publicly Perform; (II) You may not<br />
offer or impose any terms on the Adaptation that restrict the terms<br />
of the Applicable License or the ability of the recipient of the<br />
Adaptation to exercise the rights granted to that recipient under<br />
the terms of the Applicable License; (III) You must keep intact all<br />
notices that refer to the Applicable License and to the disclaimer<br />
of warranties with every copy of the Work as included in the<br />
Adaptation You Distribute or Publicly Perform; (IV) when You<br />
Distribute or Publicly Perform the Adaptation, You may not impose<br />
any effective technological measures on the Adaptation that restrict<br />
the ability of a recipient of the Adaptation from You to exercise<br />
the rights granted to that recipient under the terms of the<br />
Applicable License. This Section 4(b) applies to the Adaptation as<br />
incorporated in a Collection, but this does not require the<br />
Collection apart from the Adaptation itself to be made subject to<br />
the terms of the Applicable License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
You Distribute, or Publicly Perform the Work or any Adaptations or<br />
Collections, You must, unless a request has been made pursuant to<br />
Section 4(a), keep intact all copyright notices for the Work and<br />
provide, reasonable to the medium or means You are utilizing: (i)<br />
the name of the Original Author (or pseudonym, if applicable) if<br />
supplied, and/or if the Original Author and/or Licensor designate<br />
another party or parties (e.g., a sponsor institute, publishing<br />
entity, journal) for attribution (&quot;Attribution Parties&quot;)<br />
in Licensor's copyright notice, terms of service or by other<br />
reasonable means, the name of such party or parties; (ii) the title<br />
of the Work if supplied; (iii) to the extent reasonably practicable,<br />
the URI, if any, that Licensor specifies to be associated with the<br />
Work, unless such URI does not refer to the copyright notice or<br />
licensing information for the Work; and (iv) , consistent with<br />
Ssection 3(b), in the case of an Adaptation, a credit identifying<br />
the use of the Work in the Adaptation (e.g., &quot;French<br />
translation of the Work by Original Author,&quot; or &quot;Screenplay<br />
based on original Work by Original Author&quot;). The credit<br />
required by this Section 4(c) may be implemented in any reasonable<br />
manner; provided, however, that in the case of a Adaptation or<br />
Collection, at a minimum such credit will appear, if a credit for<br />
all contributing authors of the Adaptation or Collection appears,<br />
then as part of these credits and in a manner at least as prominent<br />
as the credits for the other contributing authors. For the avoidance<br />
of doubt, You may only use the credit required by this Section for<br />
the purpose of attribution in the manner set out above and, by<br />
exercising Your rights under this License, You may not implicitly or<br />
explicitly assert or imply any connection with, sponsorship or<br />
endorsement by the Original Author, Licensor and/or Attribution<br />
Parties, as appropriate, of You or Your use of the Work, without the<br />
separate, express prior written permission of the Original Author,<br />
Licensor and/or Attribution Parties. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Except as otherwise agreed in<br />
writing by the Licensor or as may be otherwise permitted by<br />
applicable law, if You Reproduce, Distribute or Publicly Perform the<br />
Work either by itself or as part of any Adaptations or Collections,<br />
You must not distort, mutilate, modify or take other derogatory<br />
action in relation to the Work which would be prejudicial to the<br />
Original Author's honor or reputation. Licensor agrees that in those<br />
jurisdictions (e.g. Japan), in which any exercise of the right<br />
granted in Section 3(b) of this License (the right to make<br />
Adaptations) would be deemed to be a distortion, mutilation,<br />
modification or other derogatory action prejudicial to the Original<br />
Author's honor and reputation, the Licensor will waive or not<br />
assert, as appropriate, this Section, to the fullest extent<br />
permitted by the applicable national law, to enable You to<br />
reasonably exercise Your right under Section 3(b) of this License<br />
(right to make Adaptations) but not otherwise. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>5. Representations,<br />
Warranties and Disclaimer</STRONG></P><br />
<P LANG="en-US" CLASS="western">UNLESS OTHERWISE MUTUALLY AGREED TO<br />
BY THE PARTIES IN WRITING, LICENSOR OFFERS THE WORK AS-IS AND MAKES<br />
NO REPRESENTATIONS OR WARRANTIES OF ANY KIND CONCERNING THE WORK,<br />
EXPRESS, IMPLIED, STATUTORY OR OTHERWISE, INCLUDING, WITHOUT<br />
LIMITATION, WARRANTIES OF TITLE, MERCHANTIBILITY, FITNESS FOR A<br />
PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR<br />
OTHER DEFECTS, ACCURACY, OR THE PRESENCE OF ABSENCE OF ERRORS,<br />
WHETHER OR NOT DISCOVERABLE. SOME JURISDICTIONS DO NOT ALLOW THE<br />
EXCLUSION OF IMPLIED WARRANTIES, SO SUCH EXCLUSION MAY NOT APPLY TO<br />
YOU.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>6. Limitation on Liability.</STRONG><br />
EXCEPT TO THE EXTENT REQUIRED BY APPLICABLE LAW, IN NO EVENT WILL<br />
LICENSOR BE LIABLE TO YOU ON ANY LEGAL THEORY FOR ANY SPECIAL,<br />
INCIDENTAL, CONSEQUENTIAL, PUNITIVE OR EXEMPLARY DAMAGES ARISING OUT<br />
OF THIS LICENSE OR THE USE OF THE WORK, EVEN IF LICENSOR HAS BEEN<br />
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>7. Termination</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License and the rights granted hereunder will terminate<br />
automatically upon any breach by You of the terms of this License.<br />
Individuals or entities who have received Adaptations or Collections<br />
from You under this License, however, will not have their licenses<br />
terminated provided such individuals or entities remain in full<br />
compliance with those licenses. Sections 1, 2, 5, 6, 7, and 8 will<br />
survive any termination of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Subject to the above terms and<br />
conditions, the license granted here is perpetual (for the duration<br />
of the applicable copyright in the Work). Notwithstanding the above,<br />
Licensor reserves the right to release the Work under different<br />
license terms or to stop distributing the Work at any time;<br />
provided, however that any such election will not serve to withdraw<br />
this License (or any other license that has been, or is required to<br />
be, granted under the terms of this License), and this License will<br />
continue in full force and effect unless terminated as stated above.<br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>8. Miscellaneous</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform the Work or a Collection,<br />
the Licensor offers to the recipient a license to the Work on the<br />
same terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform an Adaptation, Licensor<br />
offers to the recipient a license to the original Work on the same<br />
terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
any provision of this License is invalid or unenforceable under<br />
applicable law, it shall not affect the validity or enforceability<br />
of the remainder of the terms of this License, and without further<br />
action by the parties to this agreement, such provision shall be<br />
reformed to the minimum extent necessary to make such provision<br />
valid and enforceable. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">No<br />
term or provision of this License shall be deemed waived and no<br />
breach consented to unless such waiver or consent shall be in<br />
writing and signed by the party to be charged with such waiver or<br />
consent. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License constitutes the entire agreement between the parties with<br />
respect to the Work licensed here. There are no understandings,<br />
agreements or representations with respect to the Work not specified<br />
here. Licensor shall not be bound by any additional provisions that<br />
may appear in any communication from You. This License may not be<br />
modified without the mutual written agreement of the Licensor and<br />
You. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">The rights granted under, and<br />
the subject matter referenced, in this License were drafted<br />
utilizing the terminology of the Berne Convention for the Protection<br />
of Literary and Artistic Works (as amended on September 28, 1979),<br />
the Rome Convention of 1961, the WIPO Copyright Treaty of 1996, the<br />
WIPO Performances and Phonograms Treaty of 1996 and the Universal<br />
Copyright Convention (as revised on July 24, 1971). These rights and<br />
subject matter take effect in the relevant jurisdiction in which the<br />
License terms are sought to be enforced according to the<br />
corresponding provisions of the implementation of those treaty<br />
provisions in the applicable national law. If the standard suite of<br />
rights granted under applicable copyright law includes additional<br />
rights not granted under this License, such additional rights are<br />
deemed to be included in the License; this License is not intended<br />
to restrict the license of any rights under applicable law. <br />
</P><br />
</OL><br />
</html><br />
<br />
<br />
==Creative BioCommons NonCommercial legal code==<br />
<html><br />
<H3 LANG="en-US" CLASS="western" STYLE="line-height: 150%"><EM>License</EM></H3><br />
<P LANG="en-US" CLASS="western">THE WORK (AS DEFINED BELOW) IS<br />
PROVIDED UNDER THE TERMS OF THIS CREATIVE COMMONS PUBLIC LICENSE<br />
(&quot;CCPL&quot; OR &quot;LICENSE&quot;). THE WORK IS PROTECTED BY<br />
COPYRIGHT AND/OR OTHER APPLICABLE LAW. ANY USE OF THE WORK OTHER THAN<br />
AS AUTHORIZED UNDER THIS LICENSE OR COPYRIGHT LAW IS PROHIBITED.</P><br />
<P LANG="en-US" CLASS="western">BY EXERCISING ANY RIGHTS TO THE WORK<br />
PROVIDED HERE, YOU ACCEPT AND AGREE TO BE BOUND BY THE TERMS OF THIS<br />
LICENSE. TO THE EXTENT THIS LICENSE MAY BE CONSIDERED TO BE A<br />
CONTRACT, THE LICENSOR GRANTS YOU THE RIGHTS CONTAINED HERE IN<br />
CONSIDERATION OF YOUR ACCEPTANCE OF SUCH TERMS AND CONDITIONS.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>1. Definitions</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Adaptation&quot;</STRONG><br />
means a work based upon the Work, or upon the Work and other<br />
pre-existing works, such as a adaptation, derivative work or other<br />
alterations of a work or any other form in which the Work may be<br />
recast, transformed, or adapted including in any form recognizably<br />
derived from the original. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Collection&quot;</STRONG><br />
means a collection of nucleotide or protein sequences, either in<br />
form of physical DNA or sequence alone, which, by reason of the<br />
selection and arrangement of their contents, constitute intellectual<br />
creations, in which the Work is included in its entirety in<br />
unmodified form along with one or more other contributions, each<br />
constituting separate and independent works in themselves, which<br />
together are assembled into a collective whole. A work that<br />
constitutes a Collection will not be considered an Adaptation (as<br />
defined below) for the purposes of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Distribute&quot;</STRONG><br />
means to make available to the public the original and copies of the<br />
Work or Adaptation, as appropriate, through sale or other transfer<br />
of ownership. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;License<br />
Elements&quot;</STRONG> means the following high-level license<br />
attributes as selected by Licensor and indicated in the title of<br />
this License: Attribution, Noncommercial. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Licensor&quot;</STRONG><br />
means the individual, individuals, entity or entities that offer(s)<br />
the Work under the terms of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Original<br />
Author&quot;</STRONG> means the individual, individuals, entity or<br />
entities who created the Work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Work&quot;</STRONG><br />
means biologically meaningful sequence either in form of DNA,<br />
protein or information about DNA or protein sequence offered under<br />
the terms of this License including without limitation any<br />
production in the literary, scientific and artistic domain, whatever<br />
may be the mode or form of its expression including digital form,<br />
such as a book, and other writing; a lecture, address, sermon or<br />
other work of the same nature; an illustration, plan, sketch or<br />
three-dimensional work relative to science; a compilation of data to<br />
the extent it is protected as a copyrightable work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;You&quot;</STRONG><br />
means an individual or entity exercising rights under this License<br />
who has not previously violated the terms of this License with<br />
respect to the Work, or who has received express permission from the<br />
Licensor to exercise rights under this License despite a previous<br />
violation. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Publicly<br />
Perform&quot;</STRONG> means to perform public recitations of the<br />
Work and to communicate to the public those public recitations, by<br />
any means or process, including by wire or wireless means or public<br />
digital performances; to make available to the public Works in such<br />
a way that members of the public may access these Works from a place<br />
and at a place individually chosen by them; to perform the Work to<br />
the public by any means or process and the communication to the<br />
public of the performances of the Work, including by public digital<br />
performance; to broadcast and rebroadcast the Work by any means. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>&quot;Reproduce&quot;</STRONG><br />
means to make copies of the Work by any means including in digital<br />
form or via other electronic medium. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>2. Fair Dealing Rights.</STRONG><br />
Nothing in this License is intended to reduce, limit, or restrict any<br />
uses free from copyright or rights arising from limitations or<br />
exceptions that are provided for in connection with the copyright<br />
protection under copyright law or other applicable laws.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>3. License Grant.</STRONG><br />
Subject to the terms and conditions of this License, Licensor hereby<br />
grants You a worldwide, royalty-free, non-exclusive, perpetual (for<br />
the duration of the applicable copyright) license to exercise the<br />
rights in the Work as stated below:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Reproduce the Work, to incorporate the Work into one or more<br />
Collections, and to Reproduce the Work as incorporated in the<br />
Collections; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
create and Reproduce Adaptations provided that any such Adaptation,<br />
including any translation in any medium, takes reasonable steps to<br />
clearly label, demarcate or otherwise identify that changes were<br />
made to the original Work. For example, a translation could be<br />
marked &quot;The original work was translated from English to<br />
Spanish,&quot; or a modification could indicate &quot;The original<br />
work has been modified.&quot;; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform the Work including as incorporated<br />
in Collections; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">to Distribute and Publicly<br />
Perform Adaptations. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western">The above rights may be exercised in<br />
all media and formats whether now known or hereafter devised. The<br />
above rights include the right to make such modifications as are<br />
technically necessary to exercise the rights in other media and<br />
formats. Subject to Section 8(f), all rights not expressly granted by<br />
Licensor are hereby reserved, including but not limited to the rights<br />
described in Section 4(e).</P><br />
<P LANG="en-US" CLASS="western"><STRONG>4. Restrictions.</STRONG> The<br />
license granted in Section 3 above is expressly made subject to and<br />
limited by the following restrictions:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform the Work only under the terms of<br />
this License. You must include a copy of, or the Uniform Resource<br />
Identifier (URI) for, this License with every copy of the Work You<br />
Distribute or Publicly Perform. You may not offer or impose any<br />
terms on the Work that restrict the terms of this License or the<br />
ability of the recipient of the Work to exercise the rights granted<br />
to that recipient under the terms of the License. You may not<br />
sublicense the Work. You must keep intact all notices that refer to<br />
this License and to the disclaimer of warranties with every copy of<br />
the Work You Distribute or Publicly Perform. When You Distribute or<br />
Publicly Perform the Work, You may not impose any effective<br />
technological measures on the Work that restrict the ability of a<br />
recipient of the Work from You to exercise the rights granted to<br />
that recipient under the terms of the License. This Section 4(a)<br />
applies to the Work as incorporated in a Collection, but this does<br />
not require the Collection apart from the Work itself to be made<br />
subject to the terms of this License. If You create a Collection,<br />
upon notice from any Licensor You must, to the extent practicable,<br />
remove from the Collection any credit as required by Section 4(d),<br />
as requested. If You create an Adaptation, upon notice from any<br />
Licensor You must, to the extent practicable, remove from the<br />
Adaptation any credit as required by Section 4(d), as requested. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform an Adaptation only under: (i) the<br />
terms of this License; (ii) a later version of this License with the<br />
same License Elements as this License; (iii) a Creative Commons<br />
jurisdiction license (either this or a later license version) that<br />
contains the same License Elements as this License (e.g.,<br />
Attribution-NonCommercial 3.0 US) (&quot;Applicable License&quot;).<br />
You must include a copy of, or the URI, for Applicable License with<br />
every copy of each Adaptation You Distribute or Publicly Perform.<br />
You may not offer or impose any terms on the Adaptation that<br />
restrict the terms of the Applicable License or the ability of the<br />
recipient of the Adaptation to exercise the rights granted to that<br />
recipient under the terms of the Applicable License. You must keep<br />
intact all notices that refer to the Applicable License and to the<br />
disclaimer of warranties with every copy of the Work as included in<br />
the Adaptation You Distribute or Publicly Perform. When You<br />
Distribute or Publicly Perform the Adaptation, You may not impose<br />
any effective technological measures on the Adaptation that restrict<br />
the ability of a recipient of the Adaptation from You to exercise<br />
the rights granted to that recipient under the terms of the<br />
Applicable License. This Section 4(b) applies to the Adaptation as<br />
incorporated in a Collection, but this does not require the<br />
Collection apart from the Adaptation itself to be made subject to<br />
the terms of the Applicable License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may not exercise any of the rights granted to You in Section 3 above<br />
in any manner that is primarily intended for or directed toward<br />
commercial advantage or private monetary compensation. The exchange<br />
of the Work for other copyrighted works by means of digital<br />
file-sharing or otherwise shall not be considered to be intended for<br />
or directed toward commercial advantage or private monetary<br />
compensation, provided there is no payment of any monetary<br />
compensation in connection with the exchange of copyrighted works. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
You Distribute, or Publicly Perform the Work or any Adaptations or<br />
Collections, You must, unless a request has been made pursuant to<br />
Section 4(a), keep intact all copyright notices for the Work and<br />
provide, reasonable to the medium or means You are utilizing: (i)<br />
the name of the Original Author (or pseudonym, if applicable) if<br />
supplied, and/or if the Original Author and/or Licensor designate<br />
another party or parties (e.g., a sponsor institute, publishing<br />
entity, journal) for attribution (&quot;Attribution Parties&quot;)<br />
in Licensor's copyright notice, terms of service or by other<br />
reasonable means, the name of such party or parties; (ii) the title<br />
of the Work if supplied; (iii) to the extent reasonably practicable,<br />
the URI, if any, that Licensor specifies to be associated with the<br />
Work, unless such URI does not refer to the copyright notice or<br />
licensing information for the Work; and, (iv) consistent with<br />
Section 3(b), in the case of an Adaptation, a credit identifying the<br />
use of the Work in the Adaptation (e.g., &quot;French translation of<br />
the Work by Original Author,&quot; or &quot;Screenplay based on<br />
original Work by Original Author&quot;). The credit required by this<br />
Section 4(d) may be implemented in any reasonable manner; provided,<br />
however, that in the case of a Adaptation or Collection, at a<br />
minimum such credit will appear, if a credit for all contributing<br />
authors of the Adaptation or Collection appears, then as part of<br />
these credits and in a manner at least as prominent as the credits<br />
for the other contributing authors. For the avoidance of doubt, You<br />
may only use the credit required by this Section for the purpose of<br />
attribution in the manner set out above and, by exercising Your<br />
rights under this License, You may not implicitly or explicitly<br />
assert or imply any connection with, sponsorship or endorsement by<br />
the Original Author, Licensor and/or Attribution Parties, as<br />
appropriate, of You or Your use of the Work, without the separate,<br />
express prior written permission of the Original Author, Licensor<br />
and/or Attribution Parties. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">For the avoidance of doubt:</P><br />
<OL TYPE=i><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Non-waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme cannot be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme can be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License if Your exercise of<br />
such rights is for a purpose or use which is otherwise than<br />
noncommercial as permitted under Section 4(c) and otherwise waives<br />
the right to collect royalties through any statutory or compulsory<br />
licensing scheme; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Voluntary<br />
License Schemes</STRONG>. The Licensor reserves the right to<br />
collect royalties, whether individually or, in the event that the<br />
Licensor is a member of a collecting society that administers<br />
voluntary licensing schemes, via that society, from any exercise by<br />
You of the rights granted under this License that is for a purpose<br />
or use which is otherwise than noncommercial as permitted under<br />
Section 4(c). <br />
</P><br />
</OL><br />
<LI><P LANG="en-US" CLASS="western">Except as otherwise agreed in<br />
writing by the Licensor or as may be otherwise permitted by<br />
applicable law, if You Reproduce, Distribute or Publicly Perform the<br />
Work either by itself or as part of any Adaptations or Collections,<br />
You must not distort, mutilate, modify or take other derogatory<br />
action in relation to the Work which would be prejudicial to the<br />
Original Author's honor or reputation. Licensor agrees that in those<br />
jurisdictions (e.g. Japan), in which any exercise of the right<br />
granted in Section 3(b) of this License (the right to make<br />
Adaptations) would be deemed to be a distortion, mutilation,<br />
modification or other derogatory action prejudicial to the Original<br />
Author's honor and reputation, the Licensor will waive or not<br />
assert, as appropriate, this Section, to the fullest extent<br />
permitted by the applicable national law, to enable You to<br />
reasonably exercise Your right under Section 3(b) of this License<br />
(right to make Adaptations) but not otherwise. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>5. Representations,<br />
Warranties and Disclaimer</STRONG></P><br />
<P LANG="en-US" CLASS="western">UNLESS OTHERWISE MUTUALLY AGREED TO<br />
BY THE PARTIES IN WRITING AND TO THE FULLEST EXTENT PERMITTED BY<br />
APPLICABLE LAW, LICENSOR OFFERS THE WORK AS-IS AND MAKES NO<br />
REPRESENTATIONS OR WARRANTIES OF ANY KIND CONCERNING THE WORK,<br />
EXPRESS, IMPLIED, STATUTORY OR OTHERWISE, INCLUDING, WITHOUT<br />
LIMITATION, WARRANTIES OF TITLE, MERCHANTABILITY, FITNESS FOR A<br />
PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR<br />
OTHER DEFECTS, ACCURACY, OR THE PRESENCE OF ABSENCE OF ERRORS,<br />
WHETHER OR NOT DISCOVERABLE. SOME JURISDICTIONS DO NOT ALLOW THE<br />
EXCLUSION OF IMPLIED WARRANTIES, SO THIS EXCLUSION MAY NOT APPLY TO<br />
YOU.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>6. Limitation on Liability.</STRONG><br />
EXCEPT TO THE EXTENT REQUIRED BY APPLICABLE LAW, IN NO EVENT WILL<br />
LICENSOR BE LIABLE TO YOU ON ANY LEGAL THEORY FOR ANY SPECIAL,<br />
INCIDENTAL, CONSEQUENTIAL, PUNITIVE OR EXEMPLARY DAMAGES ARISING OUT<br />
OF THIS LICENSE OR THE USE OF THE WORK, EVEN IF LICENSOR HAS BEEN<br />
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>7. Termination</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License and the rights granted hereunder will terminate<br />
automatically upon any breach by You of the terms of this License.<br />
Individuals or entities who have received Adaptations or Collections<br />
from You under this License, however, will not have their licenses<br />
terminated provided such individuals or entities remain in full<br />
compliance with those licenses. Sections 1, 2, 5, 6, 7, and 8 will<br />
survive any termination of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Subject to the above terms and<br />
conditions, the license granted here is perpetual (for the duration<br />
of the applicable copyright in the Work). Notwithstanding the above,<br />
Licensor reserves the right to release the Work under different<br />
license terms or to stop distributing the Work at any time;<br />
provided, however that any such election will not serve to withdraw<br />
this License (or any other license that has been, or is required to<br />
be, granted under the terms of this License), and this License will<br />
continue in full force and effect unless terminated as stated above.<br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>8. Miscellaneous</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform the Work or a Collection,<br />
the Licensor offers to the recipient a license to the Work on the<br />
same terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform an Adaptation, Licensor<br />
offers to the recipient a license to the original Work on the same<br />
terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
any provision of this License is invalid or unenforceable under<br />
applicable law, it shall not affect the validity or enforceability<br />
of the remainder of the terms of this License, and without further<br />
action by the parties to this agreement, such provision shall be<br />
reformed to the minimum extent necessary to make such provision<br />
valid and enforceable. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">No<br />
term or provision of this License shall be deemed waived and no<br />
breach consented to unless such waiver or consent shall be in<br />
writing and signed by the party to be charged with such waiver or<br />
consent. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License constitutes the entire agreement between the parties with<br />
respect to the Work licensed here. There are no understandings,<br />
agreements or representations with respect to the Work not specified<br />
here. Licensor shall not be bound by any additional provisions that<br />
may appear in any communication from You. This License may not be<br />
modified without the mutual written agreement of the Licensor and<br />
You. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">The rights granted under, and<br />
the subject matter referenced, in this License were drafted<br />
utilizing the terminology of the Berne Convention for the Protection<br />
of Literary and Artistic Works (as amended on September 28, 1979),<br />
the Rome Convention of 1961, the WIPO Copyright Treaty of 1996, the<br />
WIPO Performances and Phonograms Treaty of 1996 and the Universal<br />
Copyright Convention (as revised on July 24, 1971). These rights and<br />
subject matter take effect in the relevant jurisdiction in which the<br />
License terms are sought to be enforced according to the<br />
corresponding provisions of the implementation of those treaty<br />
provisions in the applicable national law. If the standard suite of<br />
rights granted under applicable copyright law includes additional<br />
rights not granted under this License, such additional rights are<br />
deemed to be included in the License; this License is not intended<br />
to restrict the license of any rights under applicable law. <br />
</P><br />
</OL><br />
</html><br />
<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/19_July_2010Team:Warsaw/Calendar-Stage1/19 July 20102010-10-27T21:13:43Z<p>Mlower: </p>
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<br />
==Introduction==<br />
<html><br />
<img src="https://static.igem.org/mediawiki/2010/2/28/Licencja.jpg" align="right" width="200px"><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
Currently licensing of community-created biological parts is very<br />
similar to the situation on software scene before General Public<br />
License (aka. GNU GPL) was created. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
There are many great parts and devices created by the community<br />
members and released freely to the community. In addition there is<br />
very little awareness of keeping community-developed parts and their<br />
derivatives free-as-in-freedom, meaning that the work is available<br />
free of charge but can also be freely accessed and used by anyone to<br />
create new parts that still remain in the Public Domain. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
This can lead to the situation when work which was intended to<br />
remain in the Public Domain would be patented as a part of some<br />
project and the results of this project would not be returned to the<br />
community. Moreover this allows the situation in which it's legal to<br />
remove original author attribution information from parts containing<br />
elements developed by others.</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
<BR><br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
We recommend that all biological parts released to the community<br />
should be licensed in a way that ensures that the original part as<br />
well as all other parts using it can be obtained, used and modified<br />
without any limitations. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
We also recommend that single licensing scheme based on Creative<br />
BioCommons licenses (see below), a Creative Commons derivative<br />
applied to biological parts, should be adopted by synthetic biology<br />
community. <br />
</P><br />
<P LANG="en-US" CLASS="western" ALIGN=JUSTIFY STYLE="margin-bottom: 0cm; line-height: 150%"><br />
This will ensure that original author attribution information has to<br />
be included when a new part based on the work of others is developed.<br />
Moreover Creative BioCommons license is inherent, meaning that all<br />
derivatives of the work (ie. BioBrick part) licensed under BioCommons<br />
have to be licensed alike. In simple words this gives a legal<br />
warranty that all You create with intention to share with others will<br />
remain in the community. Additionally it ensures acknowledgment of<br />
Your authorship.</P><br />
</html><br />
<br />
==BioCommons in plain words ==<br />
<br />
For all those who are not lawyers here is simple explanation of CreativeBiocommons features.<br />
<br />
Both BioCommons and BioCommons non-commercial are based on Creative Commons license texts with definitions updated to apply to biological parts.<br />
<br />
===Creative BioCommons license allows===<br />
*free-as-in-freedom usage, modification and distribution of biological parts covered by this license<br />
*BioCommons allows commercial usage of covered materials, BioCommons non-commercial forbids it<br />
<br />
===as long as===<br />
*parts that are build upon elements licensed under BioCommons are licensed alike (licensing BioCommons is perpetual)<br />
*original information about authors of all subparts is retained<br />
*information about parts is distributed with link to BioCommons text<br />
<br />
===it is forbidden to===<br />
*change licensing of parts built upon BioCommons licensed parts to less open one. License must remain Creative Commons compatible (currently Biocommons or BioCommons non-commenrcial)<br />
*remove information about authors<br />
*refuse others access to parts built upon Biocommmons licensed parts<br />
<br />
==Creative BioCommons legal code==<br />
<html><br />
<H3 LANG="en-US" CLASS="western" STYLE="line-height: 150%"><EM>License</EM></H3><br />
<P LANG="en-US" CLASS="western">THE WORK (AS DEFINED BELOW) IS<br />
PROVIDED UNDER THE TERMS OF THIS CREATIVE BIOCOMMONS PUBLIC LICENSE<br />
(&quot;CBCPL&quot; OR &quot;LICENSE&quot;). THE WORK IS PROTECTED BY<br />
COPYRIGHT AND/OR OTHER APPLICABLE LAW. ANY USE OF THE WORK OTHER THAN<br />
AS AUTHORIZED UNDER THIS LICENSE OR COPYRIGHT LAW IS PROHIBITED.</P><br />
<P LANG="en-US" CLASS="western">BY EXERCISING ANY RIGHTS TO THE WORK<br />
PROVIDED HERE, YOU ACCEPT AND AGREE TO BE BOUND BY THE TERMS OF THIS<br />
LICENSE. TO THE EXTENT THIS LICENSE MAY BE CONSIDERED TO BE A<br />
CONTRACT, THE LICENSOR GRANTS YOU THE RIGHTS CONTAINED HERE IN<br />
CONSIDERATION OF YOUR ACCEPTANCE OF SUCH TERMS AND CONDITIONS.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>1. Definitions</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Adaptation&quot;</STRONG><br />
means a work based upon the Work, or upon the Work and other<br />
pre-existing works, such as a adaptation, derivative work or other<br />
alterations of a work or any other form in which the Work may be<br />
recast, transformed, or adapted including in any form recognizably<br />
derived from the original. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Collection&quot;</STRONG><br />
means a collection of nucleotide or protein sequences, either in<br />
form of physical DNA or sequence alone, which, by reason of the<br />
selection and arrangement of their contents, constitute intellectual<br />
creations, in which the Work is included in its entirety in<br />
unmodified form along with one or more other contributions, each<br />
constituting separate and independent works in themselves, which<br />
together are assembled into a collective whole. A work that<br />
constitutes a Collection will not be considered an Adaptation (as<br />
defined below) for the purposes of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Creative<br />
Commons Compatible License&quot;</STRONG> means a license that is<br />
listed at http://creativecommons.org/compatiblelicenses that has<br />
been approved by Creative Commons as being essentially equivalent to<br />
this License, including, at a minimum, because that license: (i)<br />
contains terms that have the same purpose, meaning and effect as the<br />
License Elements of this License; and, (ii) explicitly permits the<br />
relicensing of adaptations of works made available under that<br />
license under this License or a Creative Commons jurisdiction<br />
license with the same License Elements as this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Distribute&quot;</STRONG><br />
means to make available to the public the original and copies of the<br />
Work or Adaptation, as appropriate, through sale or other transfer<br />
of ownership. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;License<br />
Elements&quot;</STRONG> means the following high-level license<br />
attributes as selected by Licensor and indicated in the title of<br />
this License: Attribution. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Licensor&quot;</STRONG><br />
means the individual, individuals, entity or entities that offer(s)<br />
the Work under the terms of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Original<br />
Author&quot;</STRONG> means the individual, individuals, entity or<br />
entities who created the Work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Work&quot;</STRONG><br />
means biologically meaningful sequence either in form of DNA,<br />
protein or information about DNA or protein sequence offered under<br />
the terms of this License including without limitation any<br />
production in the literary, scientific and artistic domain, whatever<br />
may be the mode or form of its expression including digital form,<br />
such as a book, and other writing; a lecture, address, sermon or<br />
other work of the same nature; an illustration, plan, sketch or<br />
three-dimensional work relative to science; a compilation of data to<br />
the extent it is protected as a copyrightable work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;You&quot;</STRONG><br />
means an individual or entity exercising rights under this License<br />
who has not previously violated the terms of this License with<br />
respect to the Work, or who has received express permission from the<br />
Licensor to exercise rights under this License despite a previous<br />
violation. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Publicly<br />
Perform&quot;</STRONG> means to perform public recitations of the<br />
Work and to communicate to the public those public recitations, by<br />
any means or process, including by wire or wireless means or public<br />
digital performances; to make available to the public Works in such<br />
a way that members of the public may access these Works from a place<br />
and at a place individually chosen by them; to perform the Work to<br />
the public by any means or process and the communication to the<br />
public of the performances of the Work, including by public digital<br />
performance; to broadcast and rebroadcast the Work by any means. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>&quot;Reproduce&quot;</STRONG><br />
means to make copies of the Work by any means including in digital<br />
form or via other electronic medium. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>2. Fair Dealing Rights.</STRONG><br />
Nothing in this License is intended to reduce, limit, or restrict any<br />
uses free from copyright or rights arising from limitations or<br />
exceptions that are provided for in connection with the copyright<br />
protection under copyright law or other applicable laws.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>3. License Grant.</STRONG><br />
Subject to the terms and conditions of this License, Licensor hereby<br />
grants You a worldwide, royalty-free, non-exclusive, perpetual (for<br />
the duration of the applicable copyright) license to exercise the<br />
rights in the Work as stated below:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Reproduce the Work, to incorporate the Work into one or more<br />
Collections, and to Reproduce the Work as incorporated in the<br />
Collections; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
create and Reproduce Adaptations provided that any such Adaptation,<br />
including any translation in any medium, takes reasonable steps to<br />
clearly label, demarcate or otherwise identify that changes were<br />
made to the original Work. For example, a translation could be<br />
marked &quot;The original work was translated from English to<br />
Spanish,&quot; or a modification could indicate &quot;The original<br />
work has been modified.&quot;; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform the Work including as incorporated<br />
in Collections; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform Adaptations. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">For the avoidance of doubt:</P><br />
<OL TYPE=i><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Non-waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme cannot be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme can be waived, the Licensor waives the<br />
exclusive right to collect such royalties for any exercise by You<br />
of the rights granted under this License; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>Voluntary License<br />
Schemes</STRONG>. The Licensor waives the right to collect<br />
royalties, whether individually or, in the event that the Licensor<br />
is a member of a collecting society that administers voluntary<br />
licensing schemes, via that society, from any exercise by You of<br />
the rights granted under this License. <br />
</P><br />
</OL><br />
</OL><br />
<P LANG="en-US" CLASS="western">The above rights may be exercised in<br />
all media and formats whether now known or hereafter devised. The<br />
above rights include the right to make such modifications as are<br />
technically necessary to exercise the rights in other media and<br />
formats. Subject to Section 8(f), all rights not expressly granted by<br />
Licensor are hereby reserved.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>4. Restrictions.</STRONG> The<br />
license granted in Section 3 above is expressly made subject to and<br />
limited by the following restrictions:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform the Work only under the terms of<br />
this License. You must include a copy of, or the Uniform Resource<br />
Identifier (URI) for, this License with every copy of the Work You<br />
Distribute or Publicly Perform. You may not offer or impose any<br />
terms on the Work that restrict the terms of this License or the<br />
ability of the recipient of the Work to exercise the rights granted<br />
to that recipient under the terms of the License. You may not<br />
sublicense the Work. You must keep intact all notices that refer to<br />
this License and to the disclaimer of warranties with every copy of<br />
the Work You Distribute or Publicly Perform. When You Distribute or<br />
Publicly Perform the Work, You may not impose any effective<br />
technological measures on the Work that restrict the ability of a<br />
recipient of the Work from You to exercise the rights granted to<br />
that recipient under the terms of the License. This Section 4(a)<br />
applies to the Work as incorporated in a Collection, but this does<br />
not require the Collection apart from the Work itself to be made<br />
subject to the terms of this License. If You create a Collection,<br />
upon notice from any Licensor You must, to the extent practicable,<br />
remove from the Collection any credit as required by Section 4(c),<br />
as requested. If You create an Adaptation, upon notice from any<br />
Licensor You must, to the extent practicable, remove from the<br />
Adaptation any credit as required by Section 4(c), as requested. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform an Adaptation only under the<br />
terms of: (i) this License; (ii) a later version of this License<br />
with the same License Elements as this License; (iii) a Creative<br />
Commons Compatible License. If you license the Adaptation under one<br />
of the licenses mentioned in (iii), you must comply with the terms<br />
of that license. If you license the Adaptation under the terms of<br />
any of the licenses mentioned in (i), (ii) or (iii) (the &quot;Applicable<br />
License&quot;), you must comply with the terms of the Applicable<br />
License generally and the following provisions: (I) You must include<br />
a copy of, or the URI for, the Applicable License with every copy of<br />
each Adaptation You Distribute or Publicly Perform; (II) You may not<br />
offer or impose any terms on the Adaptation that restrict the terms<br />
of the Applicable License or the ability of the recipient of the<br />
Adaptation to exercise the rights granted to that recipient under<br />
the terms of the Applicable License; (III) You must keep intact all<br />
notices that refer to the Applicable License and to the disclaimer<br />
of warranties with every copy of the Work as included in the<br />
Adaptation You Distribute or Publicly Perform; (IV) when You<br />
Distribute or Publicly Perform the Adaptation, You may not impose<br />
any effective technological measures on the Adaptation that restrict<br />
the ability of a recipient of the Adaptation from You to exercise<br />
the rights granted to that recipient under the terms of the<br />
Applicable License. This Section 4(b) applies to the Adaptation as<br />
incorporated in a Collection, but this does not require the<br />
Collection apart from the Adaptation itself to be made subject to<br />
the terms of the Applicable License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
You Distribute, or Publicly Perform the Work or any Adaptations or<br />
Collections, You must, unless a request has been made pursuant to<br />
Section 4(a), keep intact all copyright notices for the Work and<br />
provide, reasonable to the medium or means You are utilizing: (i)<br />
the name of the Original Author (or pseudonym, if applicable) if<br />
supplied, and/or if the Original Author and/or Licensor designate<br />
another party or parties (e.g., a sponsor institute, publishing<br />
entity, journal) for attribution (&quot;Attribution Parties&quot;)<br />
in Licensor's copyright notice, terms of service or by other<br />
reasonable means, the name of such party or parties; (ii) the title<br />
of the Work if supplied; (iii) to the extent reasonably practicable,<br />
the URI, if any, that Licensor specifies to be associated with the<br />
Work, unless such URI does not refer to the copyright notice or<br />
licensing information for the Work; and (iv) , consistent with<br />
Ssection 3(b), in the case of an Adaptation, a credit identifying<br />
the use of the Work in the Adaptation (e.g., &quot;French<br />
translation of the Work by Original Author,&quot; or &quot;Screenplay<br />
based on original Work by Original Author&quot;). The credit<br />
required by this Section 4(c) may be implemented in any reasonable<br />
manner; provided, however, that in the case of a Adaptation or<br />
Collection, at a minimum such credit will appear, if a credit for<br />
all contributing authors of the Adaptation or Collection appears,<br />
then as part of these credits and in a manner at least as prominent<br />
as the credits for the other contributing authors. For the avoidance<br />
of doubt, You may only use the credit required by this Section for<br />
the purpose of attribution in the manner set out above and, by<br />
exercising Your rights under this License, You may not implicitly or<br />
explicitly assert or imply any connection with, sponsorship or<br />
endorsement by the Original Author, Licensor and/or Attribution<br />
Parties, as appropriate, of You or Your use of the Work, without the<br />
separate, express prior written permission of the Original Author,<br />
Licensor and/or Attribution Parties. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Except as otherwise agreed in<br />
writing by the Licensor or as may be otherwise permitted by<br />
applicable law, if You Reproduce, Distribute or Publicly Perform the<br />
Work either by itself or as part of any Adaptations or Collections,<br />
You must not distort, mutilate, modify or take other derogatory<br />
action in relation to the Work which would be prejudicial to the<br />
Original Author's honor or reputation. Licensor agrees that in those<br />
jurisdictions (e.g. Japan), in which any exercise of the right<br />
granted in Section 3(b) of this License (the right to make<br />
Adaptations) would be deemed to be a distortion, mutilation,<br />
modification or other derogatory action prejudicial to the Original<br />
Author's honor and reputation, the Licensor will waive or not<br />
assert, as appropriate, this Section, to the fullest extent<br />
permitted by the applicable national law, to enable You to<br />
reasonably exercise Your right under Section 3(b) of this License<br />
(right to make Adaptations) but not otherwise. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>5. Representations,<br />
Warranties and Disclaimer</STRONG></P><br />
<P LANG="en-US" CLASS="western">UNLESS OTHERWISE MUTUALLY AGREED TO<br />
BY THE PARTIES IN WRITING, LICENSOR OFFERS THE WORK AS-IS AND MAKES<br />
NO REPRESENTATIONS OR WARRANTIES OF ANY KIND CONCERNING THE WORK,<br />
EXPRESS, IMPLIED, STATUTORY OR OTHERWISE, INCLUDING, WITHOUT<br />
LIMITATION, WARRANTIES OF TITLE, MERCHANTIBILITY, FITNESS FOR A<br />
PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR<br />
OTHER DEFECTS, ACCURACY, OR THE PRESENCE OF ABSENCE OF ERRORS,<br />
WHETHER OR NOT DISCOVERABLE. SOME JURISDICTIONS DO NOT ALLOW THE<br />
EXCLUSION OF IMPLIED WARRANTIES, SO SUCH EXCLUSION MAY NOT APPLY TO<br />
YOU.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>6. Limitation on Liability.</STRONG><br />
EXCEPT TO THE EXTENT REQUIRED BY APPLICABLE LAW, IN NO EVENT WILL<br />
LICENSOR BE LIABLE TO YOU ON ANY LEGAL THEORY FOR ANY SPECIAL,<br />
INCIDENTAL, CONSEQUENTIAL, PUNITIVE OR EXEMPLARY DAMAGES ARISING OUT<br />
OF THIS LICENSE OR THE USE OF THE WORK, EVEN IF LICENSOR HAS BEEN<br />
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>7. Termination</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License and the rights granted hereunder will terminate<br />
automatically upon any breach by You of the terms of this License.<br />
Individuals or entities who have received Adaptations or Collections<br />
from You under this License, however, will not have their licenses<br />
terminated provided such individuals or entities remain in full<br />
compliance with those licenses. Sections 1, 2, 5, 6, 7, and 8 will<br />
survive any termination of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Subject to the above terms and<br />
conditions, the license granted here is perpetual (for the duration<br />
of the applicable copyright in the Work). Notwithstanding the above,<br />
Licensor reserves the right to release the Work under different<br />
license terms or to stop distributing the Work at any time;<br />
provided, however that any such election will not serve to withdraw<br />
this License (or any other license that has been, or is required to<br />
be, granted under the terms of this License), and this License will<br />
continue in full force and effect unless terminated as stated above.<br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>8. Miscellaneous</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform the Work or a Collection,<br />
the Licensor offers to the recipient a license to the Work on the<br />
same terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform an Adaptation, Licensor<br />
offers to the recipient a license to the original Work on the same<br />
terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
any provision of this License is invalid or unenforceable under<br />
applicable law, it shall not affect the validity or enforceability<br />
of the remainder of the terms of this License, and without further<br />
action by the parties to this agreement, such provision shall be<br />
reformed to the minimum extent necessary to make such provision<br />
valid and enforceable. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">No<br />
term or provision of this License shall be deemed waived and no<br />
breach consented to unless such waiver or consent shall be in<br />
writing and signed by the party to be charged with such waiver or<br />
consent. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License constitutes the entire agreement between the parties with<br />
respect to the Work licensed here. There are no understandings,<br />
agreements or representations with respect to the Work not specified<br />
here. Licensor shall not be bound by any additional provisions that<br />
may appear in any communication from You. This License may not be<br />
modified without the mutual written agreement of the Licensor and<br />
You. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">The rights granted under, and<br />
the subject matter referenced, in this License were drafted<br />
utilizing the terminology of the Berne Convention for the Protection<br />
of Literary and Artistic Works (as amended on September 28, 1979),<br />
the Rome Convention of 1961, the WIPO Copyright Treaty of 1996, the<br />
WIPO Performances and Phonograms Treaty of 1996 and the Universal<br />
Copyright Convention (as revised on July 24, 1971). These rights and<br />
subject matter take effect in the relevant jurisdiction in which the<br />
License terms are sought to be enforced according to the<br />
corresponding provisions of the implementation of those treaty<br />
provisions in the applicable national law. If the standard suite of<br />
rights granted under applicable copyright law includes additional<br />
rights not granted under this License, such additional rights are<br />
deemed to be included in the License; this License is not intended<br />
to restrict the license of any rights under applicable law. <br />
</P><br />
</OL><br />
</html><br />
<br />
<br />
==Creative BioCommons NonCommercial legal code==<br />
<html><br />
<H3 LANG="en-US" CLASS="western" STYLE="line-height: 150%"><EM>License</EM></H3><br />
<P LANG="en-US" CLASS="western">THE WORK (AS DEFINED BELOW) IS<br />
PROVIDED UNDER THE TERMS OF THIS CREATIVE COMMONS PUBLIC LICENSE<br />
(&quot;CCPL&quot; OR &quot;LICENSE&quot;). THE WORK IS PROTECTED BY<br />
COPYRIGHT AND/OR OTHER APPLICABLE LAW. ANY USE OF THE WORK OTHER THAN<br />
AS AUTHORIZED UNDER THIS LICENSE OR COPYRIGHT LAW IS PROHIBITED.</P><br />
<P LANG="en-US" CLASS="western">BY EXERCISING ANY RIGHTS TO THE WORK<br />
PROVIDED HERE, YOU ACCEPT AND AGREE TO BE BOUND BY THE TERMS OF THIS<br />
LICENSE. TO THE EXTENT THIS LICENSE MAY BE CONSIDERED TO BE A<br />
CONTRACT, THE LICENSOR GRANTS YOU THE RIGHTS CONTAINED HERE IN<br />
CONSIDERATION OF YOUR ACCEPTANCE OF SUCH TERMS AND CONDITIONS.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>1. Definitions</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Adaptation&quot;</STRONG><br />
means a work based upon the Work, or upon the Work and other<br />
pre-existing works, such as a adaptation, derivative work or other<br />
alterations of a work or any other form in which the Work may be<br />
recast, transformed, or adapted including in any form recognizably<br />
derived from the original. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Collection&quot;</STRONG><br />
means a collection of nucleotide or protein sequences, either in<br />
form of physical DNA or sequence alone, which, by reason of the<br />
selection and arrangement of their contents, constitute intellectual<br />
creations, in which the Work is included in its entirety in<br />
unmodified form along with one or more other contributions, each<br />
constituting separate and independent works in themselves, which<br />
together are assembled into a collective whole. A work that<br />
constitutes a Collection will not be considered an Adaptation (as<br />
defined below) for the purposes of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Distribute&quot;</STRONG><br />
means to make available to the public the original and copies of the<br />
Work or Adaptation, as appropriate, through sale or other transfer<br />
of ownership. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;License<br />
Elements&quot;</STRONG> means the following high-level license<br />
attributes as selected by Licensor and indicated in the title of<br />
this License: Attribution, Noncommercial. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Licensor&quot;</STRONG><br />
means the individual, individuals, entity or entities that offer(s)<br />
the Work under the terms of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Original<br />
Author&quot;</STRONG> means the individual, individuals, entity or<br />
entities who created the Work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Work&quot;</STRONG><br />
means biologically meaningful sequence either in form of DNA,<br />
protein or information about DNA or protein sequence offered under<br />
the terms of this License including without limitation any<br />
production in the literary, scientific and artistic domain, whatever<br />
may be the mode or form of its expression including digital form,<br />
such as a book, and other writing; a lecture, address, sermon or<br />
other work of the same nature; an illustration, plan, sketch or<br />
three-dimensional work relative to science; a compilation of data to<br />
the extent it is protected as a copyrightable work. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;You&quot;</STRONG><br />
means an individual or entity exercising rights under this License<br />
who has not previously violated the terms of this License with<br />
respect to the Work, or who has received express permission from the<br />
Licensor to exercise rights under this License despite a previous<br />
violation. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>&quot;Publicly<br />
Perform&quot;</STRONG> means to perform public recitations of the<br />
Work and to communicate to the public those public recitations, by<br />
any means or process, including by wire or wireless means or public<br />
digital performances; to make available to the public Works in such<br />
a way that members of the public may access these Works from a place<br />
and at a place individually chosen by them; to perform the Work to<br />
the public by any means or process and the communication to the<br />
public of the performances of the Work, including by public digital<br />
performance; to broadcast and rebroadcast the Work by any means. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western"><STRONG>&quot;Reproduce&quot;</STRONG><br />
means to make copies of the Work by any means including in digital<br />
form or via other electronic medium. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>2. Fair Dealing Rights.</STRONG><br />
Nothing in this License is intended to reduce, limit, or restrict any<br />
uses free from copyright or rights arising from limitations or<br />
exceptions that are provided for in connection with the copyright<br />
protection under copyright law or other applicable laws.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>3. License Grant.</STRONG><br />
Subject to the terms and conditions of this License, Licensor hereby<br />
grants You a worldwide, royalty-free, non-exclusive, perpetual (for<br />
the duration of the applicable copyright) license to exercise the<br />
rights in the Work as stated below:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Reproduce the Work, to incorporate the Work into one or more<br />
Collections, and to Reproduce the Work as incorporated in the<br />
Collections; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
create and Reproduce Adaptations provided that any such Adaptation,<br />
including any translation in any medium, takes reasonable steps to<br />
clearly label, demarcate or otherwise identify that changes were<br />
made to the original Work. For example, a translation could be<br />
marked &quot;The original work was translated from English to<br />
Spanish,&quot; or a modification could indicate &quot;The original<br />
work has been modified.&quot;; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">to<br />
Distribute and Publicly Perform the Work including as incorporated<br />
in Collections; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">to Distribute and Publicly<br />
Perform Adaptations. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western">The above rights may be exercised in<br />
all media and formats whether now known or hereafter devised. The<br />
above rights include the right to make such modifications as are<br />
technically necessary to exercise the rights in other media and<br />
formats. Subject to Section 8(f), all rights not expressly granted by<br />
Licensor are hereby reserved, including but not limited to the rights<br />
described in Section 4(e).</P><br />
<P LANG="en-US" CLASS="western"><STRONG>4. Restrictions.</STRONG> The<br />
license granted in Section 3 above is expressly made subject to and<br />
limited by the following restrictions:</P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform the Work only under the terms of<br />
this License. You must include a copy of, or the Uniform Resource<br />
Identifier (URI) for, this License with every copy of the Work You<br />
Distribute or Publicly Perform. You may not offer or impose any<br />
terms on the Work that restrict the terms of this License or the<br />
ability of the recipient of the Work to exercise the rights granted<br />
to that recipient under the terms of the License. You may not<br />
sublicense the Work. You must keep intact all notices that refer to<br />
this License and to the disclaimer of warranties with every copy of<br />
the Work You Distribute or Publicly Perform. When You Distribute or<br />
Publicly Perform the Work, You may not impose any effective<br />
technological measures on the Work that restrict the ability of a<br />
recipient of the Work from You to exercise the rights granted to<br />
that recipient under the terms of the License. This Section 4(a)<br />
applies to the Work as incorporated in a Collection, but this does<br />
not require the Collection apart from the Work itself to be made<br />
subject to the terms of this License. If You create a Collection,<br />
upon notice from any Licensor You must, to the extent practicable,<br />
remove from the Collection any credit as required by Section 4(d),<br />
as requested. If You create an Adaptation, upon notice from any<br />
Licensor You must, to the extent practicable, remove from the<br />
Adaptation any credit as required by Section 4(d), as requested. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may Distribute or Publicly Perform an Adaptation only under: (i) the<br />
terms of this License; (ii) a later version of this License with the<br />
same License Elements as this License; (iii) a Creative Commons<br />
jurisdiction license (either this or a later license version) that<br />
contains the same License Elements as this License (e.g.,<br />
Attribution-NonCommercial 3.0 US) (&quot;Applicable License&quot;).<br />
You must include a copy of, or the URI, for Applicable License with<br />
every copy of each Adaptation You Distribute or Publicly Perform.<br />
You may not offer or impose any terms on the Adaptation that<br />
restrict the terms of the Applicable License or the ability of the<br />
recipient of the Adaptation to exercise the rights granted to that<br />
recipient under the terms of the Applicable License. You must keep<br />
intact all notices that refer to the Applicable License and to the<br />
disclaimer of warranties with every copy of the Work as included in<br />
the Adaptation You Distribute or Publicly Perform. When You<br />
Distribute or Publicly Perform the Adaptation, You may not impose<br />
any effective technological measures on the Adaptation that restrict<br />
the ability of a recipient of the Adaptation from You to exercise<br />
the rights granted to that recipient under the terms of the<br />
Applicable License. This Section 4(b) applies to the Adaptation as<br />
incorporated in a Collection, but this does not require the<br />
Collection apart from the Adaptation itself to be made subject to<br />
the terms of the Applicable License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">You<br />
may not exercise any of the rights granted to You in Section 3 above<br />
in any manner that is primarily intended for or directed toward<br />
commercial advantage or private monetary compensation. The exchange<br />
of the Work for other copyrighted works by means of digital<br />
file-sharing or otherwise shall not be considered to be intended for<br />
or directed toward commercial advantage or private monetary<br />
compensation, provided there is no payment of any monetary<br />
compensation in connection with the exchange of copyrighted works. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
You Distribute, or Publicly Perform the Work or any Adaptations or<br />
Collections, You must, unless a request has been made pursuant to<br />
Section 4(a), keep intact all copyright notices for the Work and<br />
provide, reasonable to the medium or means You are utilizing: (i)<br />
the name of the Original Author (or pseudonym, if applicable) if<br />
supplied, and/or if the Original Author and/or Licensor designate<br />
another party or parties (e.g., a sponsor institute, publishing<br />
entity, journal) for attribution (&quot;Attribution Parties&quot;)<br />
in Licensor's copyright notice, terms of service or by other<br />
reasonable means, the name of such party or parties; (ii) the title<br />
of the Work if supplied; (iii) to the extent reasonably practicable,<br />
the URI, if any, that Licensor specifies to be associated with the<br />
Work, unless such URI does not refer to the copyright notice or<br />
licensing information for the Work; and, (iv) consistent with<br />
Section 3(b), in the case of an Adaptation, a credit identifying the<br />
use of the Work in the Adaptation (e.g., &quot;French translation of<br />
the Work by Original Author,&quot; or &quot;Screenplay based on<br />
original Work by Original Author&quot;). The credit required by this<br />
Section 4(d) may be implemented in any reasonable manner; provided,<br />
however, that in the case of a Adaptation or Collection, at a<br />
minimum such credit will appear, if a credit for all contributing<br />
authors of the Adaptation or Collection appears, then as part of<br />
these credits and in a manner at least as prominent as the credits<br />
for the other contributing authors. For the avoidance of doubt, You<br />
may only use the credit required by this Section for the purpose of<br />
attribution in the manner set out above and, by exercising Your<br />
rights under this License, You may not implicitly or explicitly<br />
assert or imply any connection with, sponsorship or endorsement by<br />
the Original Author, Licensor and/or Attribution Parties, as<br />
appropriate, of You or Your use of the Work, without the separate,<br />
express prior written permission of the Original Author, Licensor<br />
and/or Attribution Parties. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">For the avoidance of doubt:</P><br />
<OL TYPE=i><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Non-waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme cannot be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License; <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Waivable<br />
Compulsory License Schemes</STRONG>. In those jurisdictions in<br />
which the right to collect royalties through any statutory or<br />
compulsory licensing scheme can be waived, the Licensor reserves<br />
the exclusive right to collect such royalties for any exercise by<br />
You of the rights granted under this License if Your exercise of<br />
such rights is for a purpose or use which is otherwise than<br />
noncommercial as permitted under Section 4(c) and otherwise waives<br />
the right to collect royalties through any statutory or compulsory<br />
licensing scheme; and, <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm"><STRONG>Voluntary<br />
License Schemes</STRONG>. The Licensor reserves the right to<br />
collect royalties, whether individually or, in the event that the<br />
Licensor is a member of a collecting society that administers<br />
voluntary licensing schemes, via that society, from any exercise by<br />
You of the rights granted under this License that is for a purpose<br />
or use which is otherwise than noncommercial as permitted under<br />
Section 4(c). <br />
</P><br />
</OL><br />
<LI><P LANG="en-US" CLASS="western">Except as otherwise agreed in<br />
writing by the Licensor or as may be otherwise permitted by<br />
applicable law, if You Reproduce, Distribute or Publicly Perform the<br />
Work either by itself or as part of any Adaptations or Collections,<br />
You must not distort, mutilate, modify or take other derogatory<br />
action in relation to the Work which would be prejudicial to the<br />
Original Author's honor or reputation. Licensor agrees that in those<br />
jurisdictions (e.g. Japan), in which any exercise of the right<br />
granted in Section 3(b) of this License (the right to make<br />
Adaptations) would be deemed to be a distortion, mutilation,<br />
modification or other derogatory action prejudicial to the Original<br />
Author's honor and reputation, the Licensor will waive or not<br />
assert, as appropriate, this Section, to the fullest extent<br />
permitted by the applicable national law, to enable You to<br />
reasonably exercise Your right under Section 3(b) of this License<br />
(right to make Adaptations) but not otherwise. <br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>5. Representations,<br />
Warranties and Disclaimer</STRONG></P><br />
<P LANG="en-US" CLASS="western">UNLESS OTHERWISE MUTUALLY AGREED TO<br />
BY THE PARTIES IN WRITING AND TO THE FULLEST EXTENT PERMITTED BY<br />
APPLICABLE LAW, LICENSOR OFFERS THE WORK AS-IS AND MAKES NO<br />
REPRESENTATIONS OR WARRANTIES OF ANY KIND CONCERNING THE WORK,<br />
EXPRESS, IMPLIED, STATUTORY OR OTHERWISE, INCLUDING, WITHOUT<br />
LIMITATION, WARRANTIES OF TITLE, MERCHANTABILITY, FITNESS FOR A<br />
PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR<br />
OTHER DEFECTS, ACCURACY, OR THE PRESENCE OF ABSENCE OF ERRORS,<br />
WHETHER OR NOT DISCOVERABLE. SOME JURISDICTIONS DO NOT ALLOW THE<br />
EXCLUSION OF IMPLIED WARRANTIES, SO THIS EXCLUSION MAY NOT APPLY TO<br />
YOU.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>6. Limitation on Liability.</STRONG><br />
EXCEPT TO THE EXTENT REQUIRED BY APPLICABLE LAW, IN NO EVENT WILL<br />
LICENSOR BE LIABLE TO YOU ON ANY LEGAL THEORY FOR ANY SPECIAL,<br />
INCIDENTAL, CONSEQUENTIAL, PUNITIVE OR EXEMPLARY DAMAGES ARISING OUT<br />
OF THIS LICENSE OR THE USE OF THE WORK, EVEN IF LICENSOR HAS BEEN<br />
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.</P><br />
<P LANG="en-US" CLASS="western"><STRONG>7. Termination</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License and the rights granted hereunder will terminate<br />
automatically upon any breach by You of the terms of this License.<br />
Individuals or entities who have received Adaptations or Collections<br />
from You under this License, however, will not have their licenses<br />
terminated provided such individuals or entities remain in full<br />
compliance with those licenses. Sections 1, 2, 5, 6, 7, and 8 will<br />
survive any termination of this License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">Subject to the above terms and<br />
conditions, the license granted here is perpetual (for the duration<br />
of the applicable copyright in the Work). Notwithstanding the above,<br />
Licensor reserves the right to release the Work under different<br />
license terms or to stop distributing the Work at any time;<br />
provided, however that any such election will not serve to withdraw<br />
this License (or any other license that has been, or is required to<br />
be, granted under the terms of this License), and this License will<br />
continue in full force and effect unless terminated as stated above.<br />
</P><br />
</OL><br />
<P LANG="en-US" CLASS="western"><STRONG>8. Miscellaneous</STRONG></P><br />
<OL TYPE=a><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform the Work or a Collection,<br />
the Licensor offers to the recipient a license to the Work on the<br />
same terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">Each<br />
time You Distribute or Publicly Perform an Adaptation, Licensor<br />
offers to the recipient a license to the original Work on the same<br />
terms and conditions as the license granted to You under this<br />
License. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">If<br />
any provision of this License is invalid or unenforceable under<br />
applicable law, it shall not affect the validity or enforceability<br />
of the remainder of the terms of this License, and without further<br />
action by the parties to this agreement, such provision shall be<br />
reformed to the minimum extent necessary to make such provision<br />
valid and enforceable. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">No<br />
term or provision of this License shall be deemed waived and no<br />
breach consented to unless such waiver or consent shall be in<br />
writing and signed by the party to be charged with such waiver or<br />
consent. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western" STYLE="margin-bottom: 0cm">This<br />
License constitutes the entire agreement between the parties with<br />
respect to the Work licensed here. There are no understandings,<br />
agreements or representations with respect to the Work not specified<br />
here. Licensor shall not be bound by any additional provisions that<br />
may appear in any communication from You. This License may not be<br />
modified without the mutual written agreement of the Licensor and<br />
You. <br />
</P><br />
<LI><P LANG="en-US" CLASS="western">The rights granted under, and<br />
the subject matter referenced, in this License were drafted<br />
utilizing the terminology of the Berne Convention for the Protection<br />
of Literary and Artistic Works (as amended on September 28, 1979),<br />
the Rome Convention of 1961, the WIPO Copyright Treaty of 1996, the<br />
WIPO Performances and Phonograms Treaty of 1996 and the Universal<br />
Copyright Convention (as revised on July 24, 1971). These rights and<br />
subject matter take effect in the relevant jurisdiction in which the<br />
License terms are sought to be enforced according to the<br />
corresponding provisions of the implementation of those treaty<br />
provisions in the applicable national law. If the standard suite of<br />
rights granted under applicable copyright law includes additional<br />
rights not granted under this License, such additional rights are<br />
deemed to be included in the License; this License is not intended<br />
to restrict the license of any rights under applicable law. <br />
</P><br />
</OL><br />
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<hr />
<div><html><br />
<div id="main"><br />
<div id="subtabs"><br />
<br />
<ul id="ProjectLower" style="display:none;"></ul> <br />
<ul id="TeamLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Team">Team</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Attribution">Attribution</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Sponsors">Sponsors</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Acknowledgemets">Acknowledgemets</a></li><br />
</ul><br />
<ul id="Stage1Lower" style="display:none;"><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1">Idea</a></li><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Background">Background</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="Stage2Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Results">Results</a></li><br />
<br />
</ul><br />
<ul id="Stage3Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Design">Background and Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
<br />
</ul><br />
<ul id="Stage4Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="WetLabLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Parts">Parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
</ul><br />
<br />
</ul><br />
<br />
<ul id="SoftwareLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software/Program">See Biobrick Manager</a></li><br />
<br />
<br />
</ul><br />
<br />
</div><br />
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<br />
<script type="text/javascript"><br />
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<hr />
<div><html><br />
<div id="main"><br />
<div id="subtabs"><br />
<br />
<ul id="ProjectLower" style="display:none;"></ul> <br />
<ul id="TeamLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Team">Team</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Attribution">Attribution</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Sponsors">Sponsors</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Acknowledgemets">Acknowledgemets</a></li><br />
</ul><br />
<ul id="Stage1Lower" style="display:none;"><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1">Idea</a></li><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Background">Background</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="Stage2Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Results">Results</a></li><br />
<br />
</ul><br />
<ul id="Stage3Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Design">Background and Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
<br />
</ul><br />
<ul id="Stage4Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="WetLabLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Parts">Parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
</ul><br />
<br />
</ul><br />
<br />
<ul id="SoftwareLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software/Program">See Biobrick Manager</a></li><br />
<br />
<br />
</ul><br />
<br />
</div><br />
<div id="bodyarea"><br />
<br />
<script type="text/javascript"><br />
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<hr />
<div><html><br />
<div id="main"><br />
<div id="subtabs"><br />
<br />
<ul id="ProjectLower" style="display:none;"></ul> <br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Team">Team</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Attribution">Attribution</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Sponsors">Sponsors</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Acknowledgemets">Acknowledgemets</a></li><br />
</ul><br />
<ul id="Stage1Lower" style="display:none;"><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1">Idea</a></li><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Background">Background</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="Stage2Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Results">Results</a></li><br />
<br />
</ul><br />
<ul id="Stage3Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Design">Background and Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
<br />
</ul><br />
<ul id="Stage4Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="WetLabLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Parts">Parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
</ul><br />
<br />
</ul><br />
<br />
<ul id="SoftwareLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software/Program">See Biobrick Manager</a></li><br />
<br />
<br />
</ul><br />
<br />
</div><br />
<div id="bodyarea"><br />
<br />
<script type="text/javascript"><br />
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<hr />
<div><html><br />
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<div id="subtabs"><br />
<br />
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<li><a href="https://2010.igem.org/Team:Warsaw/Team">Team</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Attribution">Attribution</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Sponsors">Sponsors</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Acknowledgemets">Acknowledgemets</a></li><br />
</ul><br />
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<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1">Idea</a></li><br />
<li style="width:50;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Background">Background</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Promoters Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">RBSes Measured</a></li><br />
<li style="width:110px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/ExpressIt">ExpressIt! Vectors</a></li><br />
<li style="width:50px;"><a href="https://2010.igem.org/Team:Warsaw/Stage1/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="Stage2Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage2/Results">Results</a></li><br />
<br />
</ul><br />
<ul id="Stage3Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3">Idea</a></li><br />
<!-- <li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Background">Background</a></li> --><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Design">Background and Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of microphotographs</a></li><br />
<br />
</ul><br />
<ul id="Stage4Lower" style="display:none;"><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage4">Idea</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Background">Background</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Design">Design</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Results">Results</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Modeling">Modeling</a></li><br />
</ul><br />
<ul id="WetLabLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Parts">Parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/WetLab">Labbook</a></li><br />
<br />
<br />
</ul><br />
<br />
<ul id="HPLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Licensing">Licensing of biological parts</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP">Syntetic biology in Poland</a></li><br />
<li><a href="https://2010.igem.org/Team:Warsaw/HP/Gallery">Synthetic BioLectures gallery</a></li><br />
<li id="survey"><a href="https://2010.igem.org/Team:Warsaw/HP/survey">iGEM survey</a></li><br />
<br />
</ul><br />
<br />
</ul><br />
<br />
<ul id="SoftwareLower" style="display:none;"> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software">About Biobrick Manager</a></li> <br />
<li><a href="https://2010.igem.org/Team:Warsaw/Software/Program">See Biobrick Manager</a></li><br />
<br />
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</ul><br />
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<br />
<script type="text/javascript"><br />
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}<br />
ChangeLowerTab(ActiveTab);<br />
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table.month .heading td { padding:2px;; color:#aaa; text-align:center; font-size:100%; }<br />
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<h2>Our LabBook</h2><br />
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|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2010 | month=07 | title=Team:Warsaw/Calendar-Stage1}}<br />
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2010 | month=08 | title=Team:Warsaw/Calendar-Stage1}}<br />
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2010 | month=09 | title=Team:Warsaw/Calendar-Stage1}}<br />
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2010 | month=10 | title=Team:Warsaw/Calendar-Stage1}}<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage4/19_July_2010Team:Warsaw/Calendar-Stage4/19 July 20102010-10-27T20:28:47Z<p>Mlower: Team:Warsaw/Calendar-Stage4/19 July 2010 moved to Team:Warsaw/Calendar-Stage1/19 August 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/19 August 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/19_August_2010Team:Warsaw/Calendar-Stage1/19 August 20102010-10-27T20:28:47Z<p>Mlower: Team:Warsaw/Calendar-Stage4/19 July 2010 moved to Team:Warsaw/Calendar-Stage1/19 August 2010</p>
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<br><br><div class="note">Cloning Fluorescent proteins behind J23100 (without RBS) - continuation [Ania P.]</div><br />
<br> Repeating of transformations from friday 16 July (because there was nothing on the plates).<br />
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<br><br><div class="note">Cloning mCherry NLS behind Anderson's RBS (Ba_J61101) [Ania P., Milena, Kasia]</div><br />
<br> 1. Digest of mCherry NLS with XbaI/PstI for 3 hours. <br />
<br> 2. Electrophoresis (whole sample put on the gel).<br />
<br> There was not enough DNA to cut it out and isolate from agarose gel.<br />
<br> (zdjęcie)<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage4/16_July_2010Team:Warsaw/Calendar-Stage4/16 July 20102010-10-27T20:28:30Z<p>Mlower: Team:Warsaw/Calendar-Stage4/16 July 2010 moved to Team:Warsaw/Calendar-Stage1/16 August 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/16 August 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/16_August_2010Team:Warsaw/Calendar-Stage1/16 August 20102010-10-27T20:28:30Z<p>Mlower: Team:Warsaw/Calendar-Stage4/16 July 2010 moved to Team:Warsaw/Calendar-Stage1/16 August 2010</p>
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<div class = "note" >Expression of mCherryNLS from the distribution</div><br />
1. Transformation with 2ul DNA from the distribution.<br />
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<br><br><div class = "note" >Cloning Fluorescent proteins behind expression vector J23100-AndersonRBS sample 3.1</div><br />
1. EcoRI/Pst digest of obtained clones to check size of the constructs, samples:<br />
<br>GFPz3<br />
<br>CFP1<br />
<br>CFPz1<br />
<br>MOz1<br />
<br>mOrrange1<br />
<br>MC2<br />
<br>mCherry1<br />
<br>mCherryNLSz1<br />
<br>2. GEL<br />
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<br><br><div class = "note" >Cloning Fluorescent proteins behind J23100 (without RBS).</div><br />
<br>1. Ligation ofJ23100 with YFP, mCherry, mOrrange.<br />
<br>2. Transformation with ligation mixture<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage4/15_July_2010Team:Warsaw/Calendar-Stage4/15 July 20102010-10-27T20:28:22Z<p>Mlower: Team:Warsaw/Calendar-Stage4/15 July 2010 moved to Team:Warsaw/Calendar-Stage1/15 August 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/15 August 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/15_August_2010Team:Warsaw/Calendar-Stage1/15 August 20102010-10-27T20:28:22Z<p>Mlower: Team:Warsaw/Calendar-Stage4/15 July 2010 moved to Team:Warsaw/Calendar-Stage1/15 August 2010</p>
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<br><br><div class="note">Cloning fluorescent proteins behind expression vector 3.1</div><br />
<br>1. Alkaline lysis of the overnight cultures<br />
<br>2. Gel: undigested plasmid DNA to give initial information on plasmids' sizes.<br />
<br> All plasmids are of the same size, as expected<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage4/14_July_2010Team:Warsaw/Calendar-Stage4/14 July 20102010-10-27T20:28:14Z<p>Mlower: Team:Warsaw/Calendar-Stage4/14 July 2010 moved to Team:Warsaw/Calendar-Stage1/14 August 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/14 August 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/14_August_2010Team:Warsaw/Calendar-Stage1/14 August 20102010-10-27T20:28:14Z<p>Mlower: Team:Warsaw/Calendar-Stage4/14 July 2010 moved to Team:Warsaw/Calendar-Stage1/14 August 2010</p>
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<br><br><div class="note">*************************** </div><br />
<br>1. Set up liquid cultures from transformation plates<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage4/13_July_2010Team:Warsaw/Calendar-Stage4/13 July 20102010-10-27T20:27:46Z<p>Mlower: Team:Warsaw/Calendar-Stage4/13 July 2010 moved to Team:Warsaw/Calendar-Stage1/13 August 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/13 August 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/13_August_2010Team:Warsaw/Calendar-Stage1/13 August 20102010-10-27T20:27:46Z<p>Mlower: Team:Warsaw/Calendar-Stage4/13 July 2010 moved to Team:Warsaw/Calendar-Stage1/13 August 2010</p>
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Tuesday, 13.07<br />
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<br><br><div class="note">Cloning fluorescent proteins behind expression vector 3.1 </div><br />
<br>1. Transformation with ligation mixtures: expression vector + YFP/CFP/GFP/mOrrange/mCherry/mCherryNLS <br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage4/12_July_2010Team:Warsaw/Calendar-Stage4/12 July 20102010-10-27T20:27:03Z<p>Mlower: Team:Warsaw/Calendar-Stage4/12 July 2010 moved to Team:Warsaw/Calendar-Stage1/11 July 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/11 July 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/11_July_2010Team:Warsaw/Calendar-Stage1/11 July 20102010-10-27T20:27:03Z<p>Mlower: Team:Warsaw/Calendar-Stage4/12 July 2010 moved to Team:Warsaw/Calendar-Stage1/11 July 2010</p>
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Monday, 12 June 2010;<br />
<br />
<br><br><div class="note">Cloning fluorescent proteins behind expression vector 3.1 and behind promoter J23100</div><br />
1. Digest part part 3.1 – Anderson RBS from distribution (expression vector = promoter J23100+RBS) with SpeI and PstI<br />
<br>2. Digest fluorescent proteins with XbaI and PstI to clone into an expression vector. Fluorescent proteins:<br />
<br>- GFP E240<br />
<br>- CFP E0420<br />
<br>- YFP E0430<br />
<br>- mOrange E2050* <br />
<br>- mCherry J67102*<br />
<br>- mCherry NLS I712028<br />
<br>* can have an internal RBS. Check by cloning behind J23100.<br />
<br>3. Gel electrophoresis:<br>Samples in following order: E240, CFP E0420, YFP E0430, mOrange E2050, mCherry J67102, mCherry NLS J130012<br />
<br>4. Gel extraction and ligation of fluorescent proteins with expression vector.<br />
<div class="note"> GEL </div><br />
Samples: GFP E240, CFP E0420, YFP E0430, mOrange E2050, mCherry J67102, mCherry NLS J130012<br />
<br>Not all samples at expected size, some vectors at wrong size. <br />
<br>Looks like order of some samples is mixed. We will check it **after** transformation, because those are fluorescent proteins therefe it is easy to find the correct samples. Vector with J23100 of expexted size. <br />
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<br><img src="https://static.igem.org/mediawiki/igem.org/8/8e/12_07_FluoProteinsXbaPstandVectorSpePst3_1.JPG"/><br />
<br>______________________________________________<br />
<br>Digest conditions, used for all reactions:<br />
<br>8 μl DNA <br />
<br>2 μl BamHI buffer<br />
<br>10 μl H2O<br />
<br>0,3 μl enzymów <br />
<br>20 μl final volume<br />
<br>digest for 3h<br />
<br>______________________________________________<br />
<br>Ligation conditions, used for all reactions:<br />
<br>25ul insert DNA<br />
<br>2ul vector DNA<br />
<br>3ul ligase buffer<br />
<br>30 ul final volume<br />
<br>overnight ligation<br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/28_September_2010Team:Warsaw/Calendar-Stage2/28 September 20102010-10-27T20:25:41Z<p>Mlower: Team:Warsaw/Calendar-Stage2/28 September 2010 moved to Team:Warsaw/Calendar-Stage1/28 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/28 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/28_September_2010Team:Warsaw/Calendar-Stage1/28 September 20102010-10-27T20:25:41Z<p>Mlower: Team:Warsaw/Calendar-Stage2/28 September 2010 moved to Team:Warsaw/Calendar-Stage1/28 September 2010</p>
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<h2>Transformation of BL21 RIL strain with MinC expression plasmids</h2><br />
<h4>Kuba</h4><br />
<p align="justify">Transformation with pSB1A2-pT7-B0032-MinC was succesfull. No colonies were observed on pSB1A2-pT7-B0034-MinC plate (the latter transformation being repeated three more times in the following days, with identical results)</p><br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/27_September_2010Team:Warsaw/Calendar-Stage2/27 September 20102010-10-27T20:25:34Z<p>Mlower: Team:Warsaw/Calendar-Stage2/27 September 2010 moved to Team:Warsaw/Calendar-Stage1/27 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/27 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/27_September_2010Team:Warsaw/Calendar-Stage1/27 September 20102010-10-27T20:25:34Z<p>Mlower: Team:Warsaw/Calendar-Stage2/27 September 2010 moved to Team:Warsaw/Calendar-Stage1/27 September 2010</p>
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<h2>Transformation of BL21 RIL strain with MinC expression plasmids</h2><br />
<h4>Kuba</h4><br />
<p align="justify">pSB1A2-pT7-B0032-MinC and pSB1A2-pT7-B0034-MinC were transformed into BL21 RIL chemocompetent cells according to standard protocol. They were then plated on LA plates(+amp, +cm, +gluc)</p><br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/7_September_2010Team:Warsaw/Calendar-Stage2/7 September 20102010-10-27T20:25:19Z<p>Mlower: Team:Warsaw/Calendar-Stage2/7 September 2010 moved to Team:Warsaw/Calendar-Stage1/6 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/6 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/6_September_2010Team:Warsaw/Calendar-Stage1/6 September 20102010-10-27T20:25:19Z<p>Mlower: Team:Warsaw/Calendar-Stage2/7 September 2010 moved to Team:Warsaw/Calendar-Stage1/6 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/8 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/10_September_2010Team:Warsaw/Calendar-Stage2/10 September 20102010-10-27T20:24:59Z<p>Mlower: Team:Warsaw/Calendar-Stage2/10 September 2010 moved to Team:Warsaw/Calendar-Stage1/11 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/11 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/11_September_2010Team:Warsaw/Calendar-Stage1/11 September 20102010-10-27T20:24:59Z<p>Mlower: Team:Warsaw/Calendar-Stage2/10 September 2010 moved to Team:Warsaw/Calendar-Stage1/11 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/10 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/20_September_2010Team:Warsaw/Calendar-Stage2/20 September 20102010-10-27T20:24:47Z<p>Mlower: Team:Warsaw/Calendar-Stage2/20 September 2010 moved to Team:Warsaw/Calendar-Stage1/20 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/20 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/20_September_2010Team:Warsaw/Calendar-Stage1/20 September 20102010-10-27T20:24:47Z<p>Mlower: Team:Warsaw/Calendar-Stage2/20 September 2010 moved to Team:Warsaw/Calendar-Stage1/20 September 2010</p>
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<h2>Cloning MinC under RBS and a promoter</h2><br />
<h4>Kuba</h4><br />
<p align="justify">pSB1A2 plasmids containig T7 promoter and B0032/B0034 RBS have been obtained from Michał. They were subsequently digested with SpeI and PstI. MinC-harboring plasmid was cut with XbaI and PstI. Following a 1,5 h digest, pSB1A2-pT7-B0032/34 and MinC were purified by gel electrophoresis with Gel-out kit (A&A biotechnology). Ligation was preformed over-night, with T4 DNA ligase (Fermentas) in 18 <sup>o</sup>C and standard reaction buffer</p><br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/16_September_2010Team:Warsaw/Calendar-Stage2/16 September 20102010-10-27T20:24:35Z<p>Mlower: Team:Warsaw/Calendar-Stage2/16 September 2010 moved to Team:Warsaw/Calendar-Stage1/16 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/16 September 2010]]</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage1/16_September_2010Team:Warsaw/Calendar-Stage1/16 September 20102010-10-27T20:24:35Z<p>Mlower: Team:Warsaw/Calendar-Stage2/16 September 2010 moved to Team:Warsaw/Calendar-Stage1/16 September 2010</p>
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<h2>Mutagenesis of MinC</h2><br />
<h4>Kuba</h4><br />
<p align="justify">Ligation was transformed into DH5&#945; chemocompetent bacteria</p><br />
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{{TemplateBottom}}</div>Mlowerhttp://2010.igem.org/Team:Warsaw/Calendar-Stage2/15_September_2010Team:Warsaw/Calendar-Stage2/15 September 20102010-10-27T20:24:30Z<p>Mlower: Team:Warsaw/Calendar-Stage2/15 September 2010 moved to Team:Warsaw/Calendar-Stage1/15 September 2010</p>
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<div>#REDIRECT [[Team:Warsaw/Calendar-Stage1/15 September 2010]]</div>Mlower