Rhodamine-Conjugated Chitin Probe

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Methanol Fixation

  1. Rinse slide with ethanol and flame
  2. Rinse slide with PBS and dry with KimWipe
  3. Apply cell sample to microscope slide and let air dry
  4. Submerge in -20C absolute methanol for 5-10 min
  5. Wash 3 times with 1X TBS (submerge in 1X TBS for 5 minutes per wash)
    • Do not let cells dry for the rest of the procedure
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