http://2010.igem.org/wiki/index.php?title=Preparing_Plates&feed=atom&action=historyPreparing Plates - Revision history2024-03-29T07:40:58ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Preparing_Plates&diff=8608&oldid=prevAsamelson: /* Materials */2010-06-14T17:32:43Z<p><span class="autocomment">Materials</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For 1 L of Media:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For 1 L of Media:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10 g of Agar (for 1% Agar plates (w/v))</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10 g of Agar (for 1% Agar plates (w/v))</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">media components specific to media of interest</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">For LB plates</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> -10g Tryptone</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> -5g Yeast Extract</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> -10g NaCl</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*1 L ddH2O </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*1 L ddH2O </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sterile antibiotic stock solution (if required)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sterile antibiotic stock solution (if required)</div></td></tr>
</table>Asamelsonhttp://2010.igem.org/wiki/index.php?title=Preparing_Plates&diff=8607&oldid=prevAsamelson at 17:31, 14 June 20102010-06-14T17:31:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]</div></td></tr>
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</table>Asamelsonhttp://2010.igem.org/wiki/index.php?title=Preparing_Plates&diff=8603&oldid=prevAsamelson at 17:29, 14 June 20102010-06-14T17:29:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Store the labeled bags of plates in the cold room.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Store the labeled bags of plates in the cold room.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Adapted from the openwetware protocol</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Adapted from the openwetware protocol</div></td></tr>
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</table>Asamelsonhttp://2010.igem.org/wiki/index.php?title=Preparing_Plates&diff=8602&oldid=prevAsamelson: /* Pouring Plates */2010-06-14T17:27:38Z<p><span class="autocomment">Pouring Plates</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#*It is a good idea to label the stack of plates to indicate antibiotic.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#*It is a good idea to label the stack of plates to indicate antibiotic.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Store the plates in their original bags - upside down, so that the gel is hanging downwards (this keeps condensation off the gel).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Store the plates in their original bags - upside down, so that the gel is hanging downwards (this keeps condensation off the gel).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Label the <del class="diffchange diffchange-inline">bags </del>following rules:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Label the <ins class="diffchange diffchange-inline">plates </ins>following <ins class="diffchange diffchange-inline">these </ins>rules:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Red stripe- AMP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Red stripe- AMP</div></td></tr>
</table>Asamelsonhttp://2010.igem.org/wiki/index.php?title=Preparing_Plates&diff=8601&oldid=prevAsamelson: New page: ==Notes== *(Optional) pre-heat a H2O bath to 55 deg C. *If you don't know how to use the autoclave, ask for a demo. ==Materials== For 1 L of Media: *10 g of Agar (for 1% Agar plates...2010-06-14T17:27:18Z<p>New page: ==Notes== *(Optional) pre-heat a H2O bath to 55 deg C. *If you don't know how to use the autoclave, ask for a demo. ==Materials== For 1 L of Media: *10 g of Agar (for 1% Agar plates...</p>
<p><b>New page</b></p><div>==Notes==<br />
<br />
*(Optional) pre-heat a H2O bath to 55 deg C. <br />
*If you don't know how to use the autoclave, ask for a demo. <br />
<br />
==Materials==<br />
For 1 L of Media:<br />
*10 g of Agar (for 1% Agar plates (w/v))<br />
*media components specific to media of interest<br />
*1 L ddH2O <br />
*Sterile antibiotic stock solution (if required)<br />
<br />
==Method==<br />
===Mixing and Sterilizing Media===<br />
#Add dry materials and H2O to a flask sufficiently large to minimize boil-over. (ex.2 L flask for 1 L of media.)<br />
#(optional, really unnecessary) add a magnetic stir bar and stir the media.<br />
#Cover the top of the flask LOOSELY with aluminum foil.<br />
#Autoclave the media on the Fluid Cycle, 30 min<br />
#Remove media to 55 deg. H2O bath or room temp to cool.<br />
#Once media has cooled to ~55 deg. C, add antibiotics, if required.<br />
<br />
===Pouring Plates===<br />
<br />
#Obtain 2 sleeves of empty plates. 1 L of media will make about 40 plates.<br />
#Using sterile technique, pour the media into the plates. <br />
#*Cover the base of the plate, and then just a bit more after that.<br />
#Recap each plate upon pouring. If there are lots of bubbles in your plates (i.e., more than one or two on the edge), you can flame the plate using the small bunsen burner to eliminate bubbles. (See a demo on this). Another way to remove the fine bubbles that may be in your flask before pouring is to mist the inside of the flask with a 75% ethanol spray bottle.<br />
#Leave plates to dry and cool for a while (overnight even). <br />
#*It is a good idea to label the stack of plates to indicate antibiotic.<br />
#Store the plates in their original bags - upside down, so that the gel is hanging downwards (this keeps condensation off the gel).<br />
#Label the bags following rules:<br />
<br />
Red stripe- AMP<br />
Blue stripe- KAN<br />
Green stripe- CHLOR<br />
<br />
#Store the labeled bags of plates in the cold room.<br />
Adapted from the openwetware protocol</div>Asamelson