Normal SDS-PAGE using Tris-Glycine Gels and Electrophoresis Buffer

From 2010.igem.org

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(New page: '''Wear gloves at all times!''' Requirements: *30% acrylamide(Biorad) '''NEUROTOXIN!''' *4X separation gel buffer: 1.5M Tris.HCl, 0.4% SDS pH6.8 *4X stacking gel buffer: 0.5M Tris.HCl, 1...)
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'''Wear gloves at all times!'''
 
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Requirements:
 
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*30% acrylamide(Biorad) '''NEUROTOXIN!'''
 
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*4X separation gel buffer: 1.5M Tris.HCl, 0.4% SDS pH6.8
 
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*4X stacking gel buffer: 0.5M Tris.HCl, 1.92M glycine, 1% SDS water saturated isobutanol
 
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*TEMED( in yellow cabinet )
 
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*10% APS (Ammonium persulfate) ; prepare 10 ml and keep cold in fridge
 
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Hoeffer Mighty gel system
 

Latest revision as of 14:11, 24 August 2010

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