Korea U Seoul/5 September 2010
From 2010.igem.org
Amplification of BioBrick parts : pSB1A2 and pSB1C3
- Confirmed location : pSB1A2-BBa_E0040 (2010 Kit plate 1/ 14K) and pSB1C3-BBa_J04450 (2010 Kit plate 1/ 3A)
- 20uL suspension by autoclaved distilled water
- 3uL transformation to E. coli DH5α
- Plating to LB(Amp100), LB(Cm25)