INSA-Lyon/7 September 2010

From 2010.igem.org





September 7th


Digestion ( with the Roche enzyme) :

  1. I0500, 4O : E+S
  2. 24A, 10L : X+P


Agarose gel electrophoris for the purification of pha A and pha B from the PCR of the 06/09


Digestion (Roche) :

  1. pha A, pha C : E+S
  2. pha B : X+P


3 purification of PHL1273 in plate LB+Kan


Liquid cultur of Phasin and Curli and DNA extraction


Second purification of the 2 clones of 18A/24A (clone from the first purification)

Ligation and transformation of :

  1. 8C/14K in Backbone tet
  2. 18A/24A in backbone tet
  3. 4O/10L in backbone tet
  4. L3/18A in backbone amp
  5. 12/13 in backbone amp
  6. pha A/pha B in backbone amp








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