INSA-Lyon/6 September 2010
From 2010.igem.org
September 6th
Agarose electrophoris gel for cerification of the digestion of the 06/09 : All Ok except Phasin and Curli
PCR on pili I in order to amplify pha A and pha B :
- T°C melting of pha A : 53°C
- T°C melting of pha B : gradient between 56°C and 65°C :
Tubes 1 2 3 4 5 6 7 8 9 10 T°C melting 56 56,8 57,8 58,9 60 61 62,1 63,2 64,1 65
New digestion of pha A, pha B, L3, Phasin, Curli (with Roche enzyme)
Agarose gel electrophoris of verification :
- pha A, pha B (from the IGEM plate) : OK
- Curli, Phasin : ADN extraction OK, but No ADN in the digestion
Agarose gel electrophoris for verification of the PCR on Pili I : pha A OK, pha B OK (the better with 64,1°C for the T° of melting)
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