INSA-Lyon/3 September 2010

From 2010.igem.org





September 3rd

Digestion of Phasin (clones 1 and 2) and Curli


Agarose gel electrophoris for verification: no ADN


Second purification of curli and Phasin


PCR of Pha A (temperature of hybridation : 53°) and Pha B (temperature of hybridation : 63°


Modification of the shaking speed of the liquid culture of PHL1273 wich was at 100 rpm to 175 rpm


ADN extraction of Curli and Phasin from the 1st purification


Digestion (with the Ozyme kit) of :


  1. Curli : E+S
  2. Phasin, L3, pha B : X+P
  3. Pha A : E+S
  4. backbone Amp : E+P







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October
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Retrieved from "http://2010.igem.org/INSA-Lyon/3_September_2010"