INSA-Lyon/26 August 2010
From 2010.igem.org
August 26
Extraction and digestion :
- ligation 4O/10L
- ligation 8C/14K
- ligation 1F/24A
- ligation I0500/24A
Gel electrophoresis --> the ligations have not the lenght expected. Those ligations have to be done again.
The ligation L2+1N seems to present the right lenght of insert (gel electrophoresis). We will confirm this ligation by a PCR amplification of the insert.
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