INSA-Lyon/14 September 2010

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September 14th


Ligation :

  1. 18A+L3 in Cl backbone
  2. 14+12/13 : in Cl backbone
  3. Curli+22B in Tet backbone



Digestion :

  1. pha A/pha B (clones 7 to 10) with E
  2. 8C, I0500 : with E+S
  3. 14K, 22B : with X+P


Transformation of the plasmid with phasin-intein


Agarose gel electrophoris for verification :

  1. Clones 7 to 10 of the pha A/pha B ligation : none contain the ligation
  2. Digestion of 1F : ok
  3. I0500,24A : no DNA
  4. 4O,10L : the weight of the DNA extraction is not correct, maybe a contamination

Measure of fluorescence and DO on the PHL1273 culture

Start of 6 liquid cultures of PHL1273 waterbath at 28°C, 3 at 100 rpm, 3 at 140 rpm








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