BIOTEC Dresden/Notepad/8 September 2010

From 2010.igem.org

(Difference between revisions)
Line 21: Line 21:
A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.  
A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.  
-
<hr>
 
{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Revision as of 14:02, 24 October 2010

Parts Assembly

Ligation of the digested samples from 07.09.2010 was done. A 1:1 and 1:3 ligation was done. The ligation mix was transformed by electroporation and plated and left overnight at 37 degrees.

4a, 9b, 14f, 20f, 21f

The concentration of all the plasmid backbones containing chloramphenicol was measured by nanodrop.

Fusion Protein

A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.


July
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
August
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
September
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
October
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Share to Twitter Share to Facebook Share to Orkut Stumble It Email This More...