BIOTEC Dresden/Notepad/22 October 2010
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Fusion Protein
Miniprep of the overnight cultures of MBP in backbone.
Colony PCR for the MBP took place and 3 samples was sent to sequencing.
Our ordered gene sequence arrived, so restriction digest for the required zz-luxI was done followed by gel purification. Ligation of luxI-zz into the vector and transformation chemically took place followed by plating them overnight.
More colonies were picked from the transformed ordered gene sequence and mini prep was done to obtain more of it.
July |
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August |
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September |
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October |
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