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BIOTEC Dresden/Notepad/22 October 2010
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(New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Fusion Protein''' ...)
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(New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Fusion Protein''' ...)
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Revision as of 12:25, 23 October 2010
Fusion Protein
Miniprep of the overnight cultures of MBP in backbone.
Colony PCR for the MBP took place and 3 samples was sent to sequencing.
Our ordered gene sequence arrived, so restriction digest for the required zz-luxI was done followed by gel purification. Ligation of luxI-zz into the vector and transformation chemically took place followed by plating them overnight.
More colonies were picked from the transformed ordered gene sequence and mini prep was done to obtain more of it.
July |
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August |
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September |
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October |
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