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BIOTEC Dresden/Notepad/1 October 2010
From 2010.igem.org
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The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter: | The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter: | ||
| - | ptetR RBS LuxR Stop1 Stop2 luxRp | + | ptetR RBS LuxR Stop1 Stop2 luxRp-------RBS RFP---------RBS-luxI-Stop1-Stop2 |
Ligation product showed the expected length in a restriction analysis also sequencing results expected. | Ligation product showed the expected length in a restriction analysis also sequencing results expected. | ||
Revision as of 23:12, 4 October 2010
Assembly of the amplifier-reporter part
Part 45 (gel purified after restriction) was ligated into the linearized vector containing part 46 using the standard assembly method. The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter:
ptetR RBS LuxR Stop1 Stop2 luxRp-------RBS RFP---------RBS-luxI-Stop1-Stop2
Ligation product showed the expected length in a restriction analysis also sequencing results expected.
