BIOTEC Dresden/Notepad/15 October 2010

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Parts Assembly

The following digested parts were ligated into part 1a backbone.

6e+14f, 6e+21f

The following parts were ligated into the RBS backbone.

4a

The following parts were ligated into the ptetR backbone.

6e+21f

The ligation mix was transformed by chemical transformation method and left for 1 hour incubation after which they were plated and kept for overnight growth of colonies.

Fusion Protein

Transformation of the ligated parts: 34 and 35 into the chloramphenicol backbone


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