BIOTEC Dresden/Notepad/12 August 2010

From 2010.igem.org

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The plasmid DNA concentration from ligation (A) were measured by Nanodrop. The concentration of the following plasmids were sufficient.
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4a, 13e, 14f
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The concentration of the following plasmids were very low.
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15e, 20f, 21f
'''Fusion protein'''
'''Fusion protein'''
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[[Category:BIOTEC Dresden/Notepad/August|August]]
{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Latest revision as of 21:55, 27 October 2010


The plasmid DNA concentration from ligation (A) were measured by Nanodrop. The concentration of the following plasmids were sufficient.

4a, 13e, 14f

The concentration of the following plasmids were very low.

15e, 20f, 21f

Fusion protein

PCR purified samples were tested on agarose gel.
Restriction digest of PCR purified samples 1-8 and plasmids -pETMM43(9) and pETMMZZ(10).
Ligation :Scheme
1+4+9
2+5+9
3+10
6+4+9
7+5+9
8+10
Incubation overnight / 1 hr.


July
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August
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September
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October
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