"
BIOTEC Dresden/Notepad/10 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{Biotec_Dresden/Header}} Check Gradient PCR of all primers on plasmids on agarose gel: 23e, 25a, 27e <hr> {{Biotec_Dresden/month}} {{Biotec_Dresden/Bottom}}) |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{Biotec_Dresden/Header}} | {{Biotec_Dresden/Header}} | ||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <style type="text/css"> | ||
| + | #bodyContent p, #bodyContent pre, #bodyContent table { | ||
| + | margin:10px 30px; | ||
| + | } | ||
| + | </style> | ||
| + | </head> | ||
| + | </html> | ||
Check Gradient PCR of all primers on plasmids on agarose gel: | Check Gradient PCR of all primers on plasmids on agarose gel: | ||
| Line 5: | Line 15: | ||
23e, 25a, 27e | 23e, 25a, 27e | ||
| - | + | Except two parts 20f and 21f, all the other plates had sufficient growth of colonies. Hence, colony PCR of the same was carried out for which eight single colonies were picked for each part. | |
| + | 4a, 13e, 9b, 15e, 14f | ||
| + | |||
| + | Agarose gel electrophoresis was performed to check the positive clones from the PCR products. Unfortunately, the gel ran too long because of which we did not have any results for this gel. Hence, the same parts were again prepared for colony PCR to be done on 11.08.2010 and this time, five colonies for each part was picked. | ||
| + | |||
| + | The following parts were transformed by electroporation: | ||
| + | |||
| + | 10, 11, 42, 43 | ||
| + | |||
| + | |||
| + | The samples from the overnight ligation were also transformed by electroporation and plated. | ||
| + | |||
| + | |||
| + | <hr> | ||
| + | [[Category:BIOTEC Dresden/Notepad/August|August]] | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 21:54, 27 October 2010
Check Gradient PCR of all primers on plasmids on agarose gel:
23e, 25a, 27e
Except two parts 20f and 21f, all the other plates had sufficient growth of colonies. Hence, colony PCR of the same was carried out for which eight single colonies were picked for each part.
4a, 13e, 9b, 15e, 14f
Agarose gel electrophoresis was performed to check the positive clones from the PCR products. Unfortunately, the gel ran too long because of which we did not have any results for this gel. Hence, the same parts were again prepared for colony PCR to be done on 11.08.2010 and this time, five colonies for each part was picked.
The following parts were transformed by electroporation:
10, 11, 42, 43
The samples from the overnight ligation were also transformed by electroporation and plated.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
