"

From 2010.igem.org

	
		

	
	
		

		

			
  Home  
			
  Team  
					
			
			
			
  Project  
			
  Parts  
			
  Modeling  
			
  Notebook  
                        
  Safety  
                        
  Sponsors  

		
		
	

Quorum addiction module

• Extraction of the BBa_R0011 promoter from the iGEM plates.
• Electroporation of the first ligations in E. Coli.

Module hydrogen

• We continue the purification process for the deletion candidate
• We continue to try to obtain other deletion candidates

Module homologous recombination

• We inserted the cm and kan resistance cassette into the pSB1C3, unfortunately, the kan resistance cassette is cut by some of the enzymes of the assembly standard 10 and can therefore not be used with the pSB1C3
• We received the primers for gam and bet. We launch the PCR before the weekend.

Module heavy metals detection

• We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow
• We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow

Other

• The PCR on pSB1C3 worked but we obtained the pSB1C3 in very low concentrations