Housekeeping genes are genes that are ubiquitously expressed in all tissues and participate in the maintenance of basic cellular function ([http://en.wikipedia.org/wiki/Housekeeping_gene see more]). Eighty unmodified nucleotide sequences were obtained from GenBank and identified as housekeeping genes. The housekeeping genes originated from both SAT and NSAT sources. They were included in the database because the guidance specifically mentions that screening software should avoid false positive hits due to housekeeping genes.

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