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From 2010.igem.org

Evelyn:

Transformation of the ligation product: pduJK-NU and pduNT-UV.

Hilde:

select single colony from plate of transformation product of pduABJKNTUV

Colony PCR test for pduABJKNTUV

Fastdigest cutting: Promotor(Insert, E,S) & pduABJKNTUV(Vector, E,X)

Miniprep: plasmids of pduABJKNTUV

DNA Ligation Kit LONG: Prmotor+pduABJKNTUV (overnight)