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From 2010.igem.org

Jocelyn:

miniprep of pdu J(PSB1C7)

miniprep of pduK(PSB1C7)

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Zocean:

transformation :

[pduA+midRBS+pduB]

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LiuChang:

construction of plasmids of the new standard:

ligation of pduN+pduT

ligation of pduU+pduV

ligation of pduJ +pduK

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Evelyn:

Restriction Enzyme Cutting of pduN-U and pduN-V.

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Hilde:

miniprep of pduNTUV

Fastdigest cutting of pduNTUV(Insert,E,S)

Fastdigest cutting of pduABJK(Vector,E,X)

DNA Ligation Kit LONG of pduABJKNTUV (overnight)

SolutionI Ligation of pduABJKNTUV (overnight)