Team:UCL London/Glycerol Stock

From 2010.igem.org

UCL IGEM 2010

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Glycerol Stock

UCL-Plate2.JPG

Materials

  Glycerol
  Transformed E.coli cells.




Method

1. Add 80µL glycerol into each labelled eppendorf.

2. Add 300µL E.coli suspension into the glycerol.

3. Vortex & store at -20°C.

4. Rest of the E.coli suspension are stored in the fridge.


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