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From 2010.igem.org

Buffers and Stock solutions

Luria-bertani (LB)

For 1 liter dissolve in H2O:

Compound/Ingredient	Amount required (1L of medium)
Tryptone	10 g
Yeast extract	5 g
NaCl	10 g
Agar+	15 g

* Add agar in case a solid medium is required.

Autoclave on a liquid cycle. Once broth reaches room temperature, store in cold room.

M9

For 1 liter dissolve in H2O:

Compound/Ingredient	Amount required (1L of medium)
Na2HPO4	6 g
KH2PO4	3 g
NaCl	0.5 g
(NH4)Cl	1 g
Agar*	15 g

* Add agar in case a solid medium is required.

Once broth reaches room temperature, add to the broth in aseptic conditions the following amount of each solution:

Solution	Amount(1L of medium)
1 M MgSO4	2 mL
1 M CaCl2	0.1 mL
20% Glucose (w/v)*	10 mL
1M Thiamine-HCl	1 mL

* For M9-modified medium no glucose solution is added, instead 10 mL of filtered hydrocarbon/ hydrocarbon mixture are added per liter of medium. Be careful when adding the hydrocarbon/hydrocarbon mixture.

NOTE: Autoclave the solutions 1 M MgSO4, 1M CaCl2, 20% glucose (w/v) (EXCEPT thiamine-HCl 1M) on a liquid cycle. Filter the solution Thiamine-HCl 1M using a 0.22 μm membrane, store the solution in a cold room when is not required.

Store in cold room if the medium is not used.

Antibiotics

Antibiotic	Final concentration in the medium
Ampicillin	100 μg/mL
Kanamycin	50 μg/mL
Chlorampenicol	35 μg/mL
Tetracycline	15 μg/mL

All stock solutions must be kept at -20 ºC and used under aseptic conditions.

10x TBE Buffer

For 1 liter, dissolve in 800 mL H2O:

Compound/Ingredient	Amount required (1L of medium)
Tris	54 g
Boric Acid	27.5 g
0.5 M EDTA	4.65 g or 20 mL

Adjust volume to 1 liter with H2O